Corporate Banner
Satellite Banner
Next Gen Sequencing
Scientific Community
Become a Member | Sign in
Home>News>This Article

Automated TruSeq® Stranded Total RNA Library Preparation Method

Published: Tuesday, February 25, 2014
Last Updated: Tuesday, February 25, 2014
Bookmark and Share
Stranded Total RNA sequencing library preparation using epMotion minimizes hands-on time.

Eppendorf has announced the release of the epMotion automation method for Illumina’s TruSeq Stranded Total RNA library preparation kit for next-generation sequencing as “Illumina Qualified.”

“Illumina Qualified” indicates that Illumina’s analysis of libraries prepared with this epMotion method has shown the libraries to perform comparably to those prepared manually.

Sample preparation for next-generation sequencing (NGS) involves extensive manual processing that can affect reproducibility. Eppendorf epMotion Liquid Handling systems are important tools for labs that want to significantly increase throughput and consistency of results.

The automation of labor-intensive steps in NGS sample prep, like pipetting, mixing, temperature control and magnetic separation, brings about a standardized and streamlined process, with significantly reduced hands-on time and minimization of human errors.

“Eppendorf’s method for TruSeq Stranded Total RNA provides our customers with another great option for high throughput automation,” said Peter Fromen, Senior Director of Product Marketing at Illumina. “Eppendorf is one of the leading providers of automation to NGS users, and we are looking forward to additional methods from them in the near future.”

“The Stranded Total RNA sequencing library preparation using the epMotion minimizes our hands-on time while achieving overall high reproducibility. This streamlines and standardizes our sequencing workflow, thereby allowing for higher sequencing throughput. We are looking forward to collaborating with Eppendorf on additional automated library prep protocols,” said Dr. Wei Chen, Head of the Scientific Genomics Platform at Max Delbrück Centre for Molecular Medicine in Berlin, Germany.

“This method is tailored for 8, 16 or 24 samples of 100 to 1000 ng total RNA and gives sequencing ready libraries in 9 to 12 hours. While this is not much faster than the manual process, the hands-on time is significantly reduced as the automated process needs less than one hour preparation time in total,” said Dr. Reza Hashemi, Executive Director of Portfolio Management Liquid Handling at Eppendorf.

Additional automated methods for NGS library preparation from Illumina are currently in development.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Scientific News
New Virus Identified In Blood Supply
Scientists have discovered a new virus that can be transmitted through the blood supply.
Far-reaching Genetic Study of 1,000 UK People
300,000 gene variants from 1,000 people made publically available via F1000Research.
DNA Alterations as Among Earliest to Occur in Lung Cancer Development
Genetic footprints of precancer detectable in some blood samples.
Targeting DNA
Protein-based sensor could detect viral infection or kill cancer cells.
Genetic Sleuthing
Sabeti team applies Ebola methods to shed light on spread of Lassa fever.
Seeking “Gold Standard” Wastewater Treatments
Metagenomic analyses lend insights into how microbes break down wastewater contaminants.
Using Genetic Sequencing to Manage Cancer in Children
A team of scientists have investigated the feasibility of incorporating clinical sequencing information into the care of young cancer patients.
Big Data Tool to Reveal Immune System Role in Diseases
Researchers from the Icahn School of Medicine at Mount Sinai and Princeton University have designed a new online tool that predicts the role of key proteins and genes in diseases of the human immune system.
Next-Gen Genomic Tests Identify Brain-Eating Amoeba
New UCSF center aims to make tests more affordable and accessible to doctors.
Genetic Overlapping in Multiple Autoimmune Diseases May Suggest Common Therapies
CHOP genomics expert leads analysis of genetic architecture, with eye on repurposing existing drugs.

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos