The NEXTflex™ Cell Free DNA-Seq Kit for Illumina Library Preparation is optimized for library construction from low input amounts of circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA). This is the first kit optimized specifically for library construction from DNA isolated from cell free fluids.
The NEXTflex Cell Free DNA-Seq Kit can be used to construct Illumina-compatible libraries from 1 ng of DNA in two hours or less. This kit delivers high coverage and reduced bias, along with flexible multiplexing options.
The NEXTflex Cell Free DNA-Seq Kit also features Enhanced Adapter Ligation Technology, offering improved ligation efficiency, and resulting in library preps with a larger number of unique sequencing reads.
Bioo Scientific offers flexible multiplexing options compatible with the NEXTflex Cell Free DNA-Seq Kit. The kit is designed to be used with the NEXTflex™ DNA Barcodes.
These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters. Additionally, the NEXTflex Dual-Indexed DNA Barcodes can be used to multiplex up to 192 samples.
Protocols are available for automation on the SEQBOT™ NGS Library Prep Automation Platform and the Beckman Biomek® Laboratory Automation Workstations.