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Tuesday, November 25, 2014
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DNA Methylation Analysis – Reliable Cell Characterization in Regenerative Medicine
Uli Hoffmueller, Stephen Rapko, Udo Baron, Georg Wieczorek, Alexander Hellwag, Cornelia Krüger, Stefan Kärst, Leslie Wolfe and Sven Olek

We demonstrate that DNA methylation patterns can serve as characteristic markers to distinguish different cell types. We have identified panels of methylation markers that are specific to mesenchymal stem cells or various differentiated cell types in the mesenchymal lineage. This method of cell type identification has a number of advantages over conventional markers in that it is robust, is both qualitative and quantitative.

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EasyBeacons™ - new Probes Ideal for Realtime PCR Detection of Methylation Status of Single CpG Duplets and SNPs
K. Skadhauge, C. Nielsen & U.B. Christensen

The EasyBeacons™ presented here are based on the novel technology Intercalating Nucleic Acid, INA®, linked to a fluorophore and a quencher. INA® is composed of normal DNA nucleotides and Intercalating Pseudo Nucleotides (IPNs). The fact that the EasyBeacons™ are mostly composed of normal DNA nucleotides means that in many respects EasyBeacons™ behave like DNA based probes, allowing use of standard buffers, primers and enzymes and hence reduces the optimisation efforts.

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Utilizing High Speed Photography to Optimize Low Volume Dispensing Conditions
Mary Cornett, Mitch Gordon and Anca Rothe

In this study we use high-speed photography as a feedback mechanism for adjusting the Nanodrop instrument dispense settings to improve the positional dispense accuracy of low volume (nanoliter) drops. These same parameters can be investigated, with various fluid classes, to reduce deleterious effects on dispensing performance such as deflected streams, satellite formation, secondary pulses and drop deformation.

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Showing Results 11 - 13 of 13
Scientific News
Brain-dwelling Worm in UK Man's Head Sequenced
Tapeworm removed from UK resident's brain reveals genetic secrets of an elusive Far East parasite.
Marching to Our Own Sequences
Study finds DNA replication timing varies among people.
New Test Developed at Dana-Farber/Brigham and Women's Cancer Center
Rapid Heme Panel - A high-tech genetic test that speeds treatment decisions for blood cancer patients.
Urgent Need for Fast Dx to Help Tackle Antibiotic Resistance
£10 million Longitude Prize for an accurate, rapid, cost-effective and easy-to-use point-of-need diagnostic test for bacterial infections.
Almac Announces Launch of CLIA Validated Next Generation Sequencing Assay
P53 considered important biomarker for cancer drug discovery.
Light Shed On Genetic Architecture Of Kidney Cancer
Research reveals link between renal cell carcinoma and exposure to aristolochic acid.
Large-scale Study Raises Hopes For Development Of E. coli Vaccine
Global comparison reveals surprisingly close relation between E. coli strains that cause traveller's diarrhoea.
DNA Sequencing Helps Identify Genetic Defects In Glaucoma
Scientists from the University of Liverpool have sequenced the mitochondrial genome in glaucoma patients to help further understanding into the genetic basis for the disease.
New Genetic Cause of Epilepsy Identified
San Diego girl improves after discovery of DNA mutation tied to potassium regulation.
Environmental Carcinogens Leave Distinctive Genetic Imprints in Tumors
Chemically induced tumors bear ‘smoking gun’ traces that sharply differentiate them from genetically engineered cancers.
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