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An Efficient Method for the Incorporation of Molecular Probes at Multiple/Specific sites in RNA: Levulinyl Protection for 2'-ACE ® , 5'-Silyl Oligoribonucleotide Synthesis
Xiaoqin Cheng, Shawn Begay, Randy Rauen, Kelly Grimsley, Kaizhang He, Michael Delaney

A unique method that uses a levulinate ester as a protecting group to introduce conjugates or molecular probes to virtually any location in a synthetic RNA molecule is discussed. The Levulinyl protecting group is stable in RNA synthesis conditions and can be removed without affecting the other parts of the synthesized RNA. We show the capabilities of this approach with three high-complexity synthesis examples.

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Acoustophoretic microfluidic device for high throughput DNA sequencing
V.V Unnikuttan1, H.N Unni 1

Multiphysics modelling for acoustic standing wave technology combined with micro-technology which can be used for manipulation and concentration on typical Lab-on-Chip devices for DNA sequencing.

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Advanced Microfluidic Mixing Device for the Study of Macromolecule Dynamics
Shubha Jain, F. Azam, H.N.Unni

We have developed and characterized a micro-fluidic mixer to study the macro-molecule dynamics such as kinetics of protein folding, DNA sequencing, single molecule study and detection etc. on a micro-second timescale. It is observed that geometry of channel has significant impact on the mixing performance of channel and also mixing time. This mixer could be used for trapping, sequencing of micro and macro molecules such as cell, DNA and protein samples under flow condition

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Bovine RNA-seq data analysis of liver and pituitary gland
Pareek CS12, Smoczynski R12, Dziuba P12, Sikora M12, Golebiewski M2, Blaszczyk P12, Gelfand B3, Yaping F3, Kumar D3.

Two key applications of RNA-seq i.e., i) transcriptome read mapping to a reference genome and ii) SNP detections were investigated to analysis of bovine liver and pituitary gland transcriptome. Here, we have presented ONLY the obtained results of bovine pituitary gland.

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Whole Transcriptome RNA-SEQ with Ion Torrent Platform: FFPE, Fresh LCM and FFPE LCM Samples. Increasingly Difficult
Sara Franceschi1, Paolo Are*ni1, Marco La Ferla1, Generoso Bevilacqua1,2, Chiara Maria Mazzan*1 , Francesca Lessi1

We developed a high performance method to analyze the whole transcriptome of our FFPE samples, obtaining a very high number of reads (78,186,377 usable reads) perfectly comparable with samples with a large amount of RNA such as samples obtained from cells or fresh tissues.

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Study Of The Active Bacterial Community In Two Membrane Bioreactors
Moreno-Mesonero, L.1, Moreno, Y.1*, Morillo, J.A., Muñagorri, F.2 and Alonso, J.L.1

In this work, bacterial communities from two MBR systems treating leachates were evaluated using the 16S rRNA metagenomics approach, with and without a viability dye (Propidium Monoazide, PMA).

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Improved Small RNA Library Preparation Workflow for Next-Generation Sequencing
Sabrina Shore, Jordana Henderson, Anton McCaffrey, Gerald Zon, Richard Hogrefe

We describe an optimized workflow which suppresses adapter dimers, works with low RNA input and eliminates the need for a gel purification step.

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Innovative technology that enables RNAi in difficult to transfect cells
Christina Yamada, Kathryn Robinson, Allison St. Amand, Zaklina Strezoska, Greg Wardle, Anastasia Khvorova, Devin Leake

Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.

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Innovative technology that enables RNAi in difficult to transfect cells
Christina Yamada, Kathryn Robinson, Allison St. Amand, Zaklina Strezoska, Greg Wardle, Anastasia Khvorova, Devin Leake

Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.

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Scientific News
New Inflammatory Disease Discovered
NIH researchers have discovered a rare and potentially deadly disease - otulipenia - the mostly affects children.
Bringng NGS to the Crime Lab
New technology being validated in BCI lab for use in Ohio missing persons cases.
Expanding Knowledge of Viral Diversity
Environmental datasets help researchers double the number of microbial phyla known to be infected by viruses.
How Cloud Connectivity Can Combat the Reproducibility Crisis
This infographic explains the reproducibility crisis, and how cloud connectivity can help overcome this problem.
The Power of Model Systems
New insights into the influence of host on the gut microbiome are revealed with in situ light sheet fluorescence microscopy and stochastic mathematical modelling.
New Way To Measure Important Chemical Modification On RNA
Technology could advance stem cells’ use in regenerative medicine, UCLA researchers say.
Mapping Antibody Creation in Humans
Researchers have created the first, detailed map of the body's antibody production, which could suggest new treatment options for immune disorders.
Decoding the Genome of the Olive Tree
A team of scientists from three Spanish centers has sequenced, for the first time ever, the complete genome of the olive tree. This work will facilitate genetic improvement for production of olives and olive oil, two key products in the Spanish economy and diet.
Four Newly-Identified Genes Could Improve Rice
A Japanese research team have applied a method used in human genetic analysis to rice and rapidly discovered four new genes that are potentially significant for agriculture. These findings could influence crop breeding and help combat food shortages caused by a growing population.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
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