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Transcription Factors to Classify Tumor Types and Subtypes
Benjamin Otto 1,2, Kristin Klätschke 2, Thomas Streichert 3, Christoph Wagener 2, Genrich Tolstonog 4

Here we introduce the use of the unsupervised approach to identify transcription factors (TFs) that are specific for different tumor types.

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Identifying Molecular Signatures of Tumors Using Novel Fluorescence Resonance Energy Transfer Networks
Vishwa Nellore, Chris Dwyer

We developed FRET sensors that can detect 125 fluorophores simultaneously. From experimental analyses of over 1200 time-resolved fluorescence signatures on 300 prototypical sensors, we show that the optical responses are highly repeatable and minor variations between FRET networks can be discriminated resulting in a total of 10^375 unique responses in theory.

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NEXTflex™ qRNA-Seq™ Molecular Indexing for ChIP-Seq and RNA-Seq
Jan Risinger, Masoud Toloue Ph.D.

Most Next Generation Sequencing (NGS) library prep methods introduce sequence bias with the use of enzyme processing and fragmentation steps can introduce errors in the form of incorrect sequence and misrepresented copy number. With molecular indexed libraries, each molecule is tagged with a molecular index randomly chosen from ~10,000 combinations so that any two identical molecules become distinguishable (with odds of 10,000/1), and can be independently evaluated in later data analysis.

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A complete workflow from sample preparation to analysis using SureSelect target enrichment system for Ion Proton semiconductor sequencing
Christian Le Cocq, Kyeong Soo Jeong, Arjun Vadapalli, Joseph Ong, Elin Agne, Filip Karlsson, Ashutosh Ashutosh, Francisco Useche, Jayati Ghosh, Henrik Johansson, Scott Happe, Douglas Roberts, and Holly Hogrefe

Agilent’s SureSelect Target Enrichment for the Ion Proton Platform provides a comprehensive, efficient, robust, and cost-effective means to sequence subsets of the human genome.

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Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
Tina Lüders (1), Jens Keilwagen (2), Neele Wendler (3), Axel Himmelbach (3), Rajiv Sharma (3), Benjamin Kilian (3,4), Nils Stein (3), Frank Ordon (1)

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.

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Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
Tina Lüders (1), Jens Keilwagen (2), Neele Wendler (3), Axel Himmelbach (3), Rajiv Sharma (3), Benjamin Kilian (3,4), Nils Stein (3), Frank Ordon (1)

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.

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Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies
W. John Martin

There is an increasing incidence of diseases with accompanying signs and symptoms of brain damage. These include neurological and psychiatric illnesses, childhood behavioral disorders, and such common conditions as chronic fatigue, Gulf War Syndrome, so-called “chronic Lyme disease”, and many cancers. Altogether, these diseases have an enormous social impact.

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High-Throughput Analysis of DNA Samples using the D1K ScreenTape Assay and the Agilent 2200 TapeStation System
Arunkumar Padmanaban, Ruediger Salowsky, Adam Inche

Recent advances in genomics demands to look at a wealth of genetic information in a short period of time. DNA analysis using slab gel electrophoresis and capillary electrophoresis are widely being used as a QC step in next generation sequencing and microarray studies. However, often these techniques lack the speed and involve more manual steps to perform the assay.

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Improved Ligation Specificity with Chemically Modified Ligation Components
Sabrina Shore, Alexandre Lebedev, Elena Hidalgo Ashrafi, Gerald Zon, Natasha Paul, Richard Hogrefe

Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.

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Showing Results 11 - 20 of 24
Scientific News
Tumor-Only Genetic Sequencing May Misguide Cancer Treatment in Nearly Half of All Patients
Johns Hopkins scientists say the genetic code of tumors must be compared to patients’ noncancer genome to get a true picture.
Genetic Link For Rare Intestinal Cancer
Researchers recommend screening for people with family history.
Tumor DNA in Blood Reveals Lymphoma Progression
Using an advanced genetic test, researchers were able to detect diffuse large B-cell lymphoma (DLBCL) in blood serum before it could be seen on CT scans.
Likely Genetic Source Of Muscle Weakness Found
Simple genetic test by TGen reveals likely causes of disease, after other extensive testing failed; one child’s case produces discovery.
Mountain Gorillas Enter The Genomic Age
First in-depth analysis reveals genetic impact of long-term population decline.
Personalized Melanoma Vaccines Marshal Powerful Immune Response
Vaccines target unique mutations in each patient’s tumor.
Cell Powerhouse Sequencing Technology Provides Deeper Look at Inherited Disease Risk
A new sequencing technique may provide a clearer picture of how genes in mitochondria, the “powerhouses” that turn sugar into energy in human cells, shape each person’s inherited risk for diabetes, heart disease and cancer.
Genome Study Predicts DNA of the Whole of Iceland
Large genome databases are starting to reveal critical health information-even about people who have not contributed their DNA.
‘Atomic Chicken-Wire’ Is Key To Faster DNA Sequencing
An unusual and very exciting form of carbon - that can be created by drawing on paper- looks to hold the key to real-time, high throughput DNA sequencing, a technique that would revolutionise medical research and testing.
New Autism-Causing Genetic Variant Identified
Novel approach expected to be useful for other diseases too.
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