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DNA Methylation Analysis – Reliable Cell Characterization in Regenerative Medicine
Uli Hoffmueller, Stephen Rapko, Udo Baron, Georg Wieczorek, Alexander Hellwag, Cornelia Krüger, Stefan Kärst, Leslie Wolfe and Sven Olek

We demonstrate that DNA methylation patterns can serve as characteristic markers to distinguish different cell types. We have identified panels of methylation markers that are specific to mesenchymal stem cells or various differentiated cell types in the mesenchymal lineage. This method of cell type identification has a number of advantages over conventional markers in that it is robust, is both qualitative and quantitative.

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EasyBeacons™ - new Probes Ideal for Realtime PCR Detection of Methylation Status of Single CpG Duplets and SNPs
K. Skadhauge, C. Nielsen & U.B. Christensen

The EasyBeacons™ presented here are based on the novel technology Intercalating Nucleic Acid, INA®, linked to a fluorophore and a quencher. INA® is composed of normal DNA nucleotides and Intercalating Pseudo Nucleotides (IPNs). The fact that the EasyBeacons™ are mostly composed of normal DNA nucleotides means that in many respects EasyBeacons™ behave like DNA based probes, allowing use of standard buffers, primers and enzymes and hence reduces the optimisation efforts.

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Utilizing High Speed Photography to Optimize Low Volume Dispensing Conditions
Mary Cornett, Mitch Gordon and Anca Rothe

In this study we use high-speed photography as a feedback mechanism for adjusting the Nanodrop instrument dispense settings to improve the positional dispense accuracy of low volume (nanoliter) drops. These same parameters can be investigated, with various fluid classes, to reduce deleterious effects on dispensing performance such as deflected streams, satellite formation, secondary pulses and drop deformation.

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Showing Results 11 - 13 of 13
Scientific News
Dozens of Genes Associated with Autism in New Research
Functions of newly identified genes converge on a few important biological processes.
Genome Sequenced of Enterovirus D68
Virus has spread rapidly causing severe respiratory illness in young children, with some patients requiring hospitalization.
IDT Shares Tips for Optimizing Target Capture
The article discusses improved target capture methods for increasing reliability of next generation sequencing data.
Earliest Modern Human Sequenced
Researchers discover fragments of Neandertal DNA in the genome of a 45,000-year-old modern human from Siberia.
TGAC Leads Research To Help Identify Animal-To-Human Transmitted Diseases
The Genome Analysis Centre (TGAC) will lead research into the development of bioinformatics to support the identification and characterisation of viruses through metagenomics.
Novel Mutations Discovered In Cats Provide Insight Into Human Eye Diseases
99 Lives Cat Genome Sequencing Initiative makes discoveries using Maverix analytic platform.
ISB Gets $6.5 Million from NCI
Company has received two-year contract to create ‘Cancer Genomics Cloud’ with partners Google and SRA International.
Eureka Genomics and VeriPrime Beef Cooperative Partner
Deal to develop molecular assays to better manage cattle health risk.
Dramatic Response And Resistance To Cancer Drug Traced
Sequencing reveals why thyroid tumor responded to, and eventually resisted, treatment.
Researchers Reveal Genomic Diversity Of Individual Lung Tumors
Findings suggest sequencing a single region of a localized tumor will identify driver mutations.
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