|Homology-directed repair with Dharmacon™ Edit-R™ CRISPR-Cas9 and single-stranded DNA oligos|
John A. Schiel, Eldon T. Chou, Maren Mayer, Emily M. Anderson , and Anja van Brabant Smith | Dharmacon, now part of GE Healthcare, 2650 Crescent Drive, Suite #100, Lafayette, CO 80026, US
Here we demonstrate how to perform lipid based transfections for homology directed repair using DharmaFECT Duo, CRISPR-Cas9 reagents and, synthetic DNA donor oligos.
|Increasing gene editing efficiencies in eukaryotic cell lines by selection of appropriate CRISPR-Cas9 reagents |
Melissa L. Kelley, Žaklina Strezoska, Elena Maksimova, Hidevaldo Machado, Emily M. Anderson, Maren Mayer, Annaleen Vermeulen, Shawn McClelland, Anja van Brabant Smith
Overview of various CRISPR-Cas9 reagents to provide the highest efficiency of gene editing in your experiments.
|Knockdown of p53 by Accell self-delivering siRNA causes inhibition of p53-dependent DNA damage response in IMR-32 neuroblastoma cell line and β-amyloid toxicity in rat cortical neurons |
Žaklina Strezoska, Tamara Seredenina1, Devin Leake, Annaleen Vermeulen
Here we describe how application of Accell siRNA enabled the development of a high content screening assay in IMR-32 neuroblastoma cells and a whole culture cell viability assay in primary rat cortical neurons.
|An Efficient Method for the Incorporation of Molecular Probes at Multiple/Specific sites in RNA: Levulinyl Protection for 2'-ACE ® , 5'-Silyl Oligoribonucleotide Synthesis|
Xiaoqin Cheng, Shawn Begay, Randy Rauen, Kelly Grimsley, Kaizhang He, Michael Delaney
A unique method that uses a levulinate ester as a protecting group to introduce conjugates or molecular probes to virtually any location in a synthetic RNA molecule is discussed. The Levulinyl protecting group is stable in RNA synthesis conditions and can be removed without affecting the other parts of the synthesized RNA. We show the capabilities of this approach with three high-complexity synthesis examples.
|Acoustophoretic microfluidic device for high throughput DNA sequencing|
V.V Unnikuttan1, H.N Unni 1
Multiphysics modelling for acoustic standing wave technology combined with micro-technology which can be used for manipulation and concentration on typical Lab-on-Chip devices for DNA sequencing.
|Advanced Microfluidic Mixing Device for the Study of Macromolecule Dynamics|
Shubha Jain, F. Azam, H.N.Unni
We have developed and characterized a micro-fluidic mixer to study the macro-molecule dynamics such as kinetics of protein folding, DNA sequencing, single molecule study and detection etc. on a micro-second timescale. It is observed that geometry of channel has significant impact on the mixing performance of channel and also mixing time. This mixer could be used for trapping, sequencing of micro and macro molecules such as cell, DNA and protein samples under flow condition
|Bovine RNA-seq data analysis of liver and pituitary gland|
Pareek CS12, Smoczynski R12, Dziuba P12, Sikora M12, Golebiewski M2, Blaszczyk P12, Gelfand B3, Yaping F3, Kumar D3.
Two key applications of RNA-seq i.e., i) transcriptome read mapping to a reference genome and ii) SNP detections were investigated to analysis of bovine liver and pituitary gland transcriptome. Here, we have presented ONLY the obtained results of bovine pituitary gland.
|Whole Transcriptome RNA-SEQ with Ion Torrent Platform: FFPE, Fresh LCM and FFPE LCM Samples. Increasingly Difficult|
Sara Franceschi1, Paolo Are*ni1, Marco La Ferla1, Generoso Bevilacqua1,2, Chiara Maria Mazzan*1 , Francesca Lessi1
We developed a high performance method to analyze the whole transcriptome of our FFPE samples, obtaining a very high number of reads (78,186,377 usable reads) perfectly comparable with samples with a large amount of RNA such as samples obtained from cells or fresh tissues.
|Study Of The Active Bacterial Community In Two Membrane Bioreactors|
Moreno-Mesonero, L.1, Moreno, Y.1*, Morillo, J.A., Muñagorri, F.2 and Alonso, J.L.1
In this work, bacterial communities from two MBR systems treating leachates were evaluated using the 16S rRNA metagenomics approach, with and without a viability dye (Propidium Monoazide, PMA).