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siRNA Screening: Development of Hit Stratification Strategies
Žaklina Strezoska, Annaleen Vermeulen, Emily M. Anderson, Anja Smith, Devin Leake

This poster compares different approaches to hit stratification and validation after an initial screen. Standard siRNA reagents deconvoluted from a pooled set of four were compared to a pooled set of four specificity enhanced reagents. High confidence hits were similar. To explore the validity of low confidence hits, a chimeric approach was used whereby a gene-specific seed sequence was introduced into a non-targeting siRNA scaffold. This work resulted in new hit stratification strategies.

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The Chemical Synthesis of Long and Highly Modified RNA using 2'-ACE Chemistry
Xiaoqin Cheng, Kristina Larson, Letitia Kwok, David Mierzejewski, Shawn Begay, Randy Rauen, Kelly Grimsley, Kaizhang He, Michael Delaney, David Kitchen, Amanda Haas, Melissa Kelley, Anja van Brabant Smith

Dharmacon has previously developed a novel RNA synthesis chemistry making RNA synthesis as reliable, accessible and of comparable quality as routinely observed in DNA synthesis.

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A multiplexed amplicon sequencing technology for FFPE and circulating, cell-free DNA
Laurie Kurihara, Catherine Couture, Julie Laliberte, Sukhinder Sandhu, Jonathan Irish, Tim Harkins and Vladimir Makarov

A novel amplicon approach allowing for hundreds of amplicons to be multiplexed in a single tube with a two workflow from sample to sequencer.

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Reducing Biases in Small RNA Sequencing
Adam Morris Ph.D., Dawn Obermoeller, Masoud Toloue Ph.D.

Small RNA-Seq libraries utilizing randomized adapters demonstrated significantly less bias and more even coverage due to reductions in ligase bias.

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Amplicon Based 16S Ribosomal RNA Sequencing and Genus Identification
*Jan Risinger, *Lindsey Renken, +Josh Hill, +Noushin Ghaffari, PhD, +Richard P. Metz, PhD, +Charles D. Johnson, PhD, *Masoud M. Toloue, Ph

Rapid determination of highly complex bacterial populations through targeted amplification of 16S rRNA V1 - V3 hypervariable regions can provide an accurate gauge of diversity at taxonomic hierarchies as low as the genus level.

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Stable Chloroplast: Myth or Reality?
Shailesh Joshi and Dyfed Evans

Chloroplasts principally encode the photosynthetic machinery in Viridiplantae. It has long been accepted that in photosynthetic plants chloroplast genomic structure is uniquely stable as it is maternally and clonally inherited. The first chloroplast genomes sequenced supported this view. The current study was undertaken to address the potential issue of global chloroplast(in)stability.

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Accurate Normalization of Grain Samples Using Pressure-Based Volume Measurement Technology
Bill Gigante, John Thomas Bradshaw, Tanya Knaide

A common challenge experienced by sample screening facilities is identifying individual sample quantity prior to DNA extraction. Accurately identifying sample quantity allows the user to pipette exact amounts of extraction buffer into each well.

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Geneious R8: A Powerful and Comprehensive Suite of Molecular Biology Tools

Christian Olsen, Kashef Qaadri, Richard Moir, Matt Kearse, Simon Buxton, Matthew Cheung, Hengjie Wang, Jonas Kuhn, Steven Stones-Havas, Chris Duran

Geneious R8: A powerful and comprehensive suite of molecular biology tools.

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Putative Genes Identified on Two Growth Conditions of G. boninense
Jayanthi N, Abrizah O, Low ETL, O-Abdullah M, Hogan M, Cuomo CA, Desjardins C, Abdul Manaf MA, Rajinder S, Birren B and Ravigadevi S

Putative genes identified on two growth conditions of Ganoderma boninense.

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Scientific News
Research at St Thomas’s Hospital Exploring Causative Factors of Atopic Eczema and Food Allergy in Infants
Carsten Flohr and his research group at St Thomas’s hospital, London are currently investigating the interaction between skin and gut microbiota in relation to the associated risk of atopic eczema (AE) and food allergy in infants.
Gut Bacteria Can Dramatically Amplify Cancer Immunotherapy
Manipulating microbes maximizes tumor immunity in mice.
Proteins Crucial to Loss of Hearing Identified
Proteins play key role in genes that help auditory hair cells grow.
New Virus Identified In Blood Supply
Scientists have discovered a new virus that can be transmitted through the blood supply.
Far-reaching Genetic Study of 1,000 UK People
300,000 gene variants from 1,000 people made publically available via F1000Research.
DNA Alterations as Among Earliest to Occur in Lung Cancer Development
Genetic footprints of precancer detectable in some blood samples.
Targeting DNA
Protein-based sensor could detect viral infection or kill cancer cells.
Genetic Sleuthing
Sabeti team applies Ebola methods to shed light on spread of Lassa fever.
Seeking “Gold Standard” Wastewater Treatments
Metagenomic analyses lend insights into how microbes break down wastewater contaminants.
Using Genetic Sequencing to Manage Cancer in Children
A team of scientists have investigated the feasibility of incorporating clinical sequencing information into the care of young cancer patients.
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