Advanced Microfluidic Mixing Device for the Study of Macromolecule Dynamics

We have demonstrated a microfluidic mixer device to characterized and study the macromolecule dynamics such as kinetics of protein folding, DNA/RNA sequencing, single molecule study and detection etc. Furthermore by integrating the concept of microfluidic mixer with magnetic beads, make feasible to study the next-generation sequencing applications like genomic DNA sequencing of microbial species from single cell and protein RNA interaction. We applied the concept of geometric variation such as length, height, geometric shape and number of channel to enhance the mixing efficiency without applying any external force or energy called passive mixing. To control the viscous force low Reynolds number is considered, thus the flow would be laminar. As a result we achieved the complete mixing at the latest reservoir. The Comsol Multiphysics platform has been used to study the simulation work by coupling of convective-diffusion and Navier-Stokes equation. In addition, applications of microfluidics technology use in Lab on chip devices and also span biomedical and healthcare field enormously.