More and more recently published chemistry examples in glass-chip- and Teflon based microreactors begin to exploit the possibility of superheating solvents for process intensification leading to drastically shortened reaction times.

The Good Manufacturing Practice (GMP) state that the active pharmaceutical ingredient (API) intended for use in early stage clinical trials should be of "suitable" quality. The Clinic Ready quality standard ensures that the API is synthesised with all the appropriate documentation to facilitate QP release of the final IMP for guman clinical dosing.

Using novel SiC-technology to mimic conventional heated autoclave experiments.

The formation of benzyl azide by diazotransfer to benzyl amine was screened and subsequently scaled up using continuous flow chemistry.

Matrix elimination is a method of sample preparation that removes certain matrix species from a sample, typically by specifically binding matrix species to a solid phase. Interfering compounds can be removed before the analyte determination, thus improving the quality of results as well as lengthening the lifetime of consumables such as columns.

Stability of noncovalent complexes of VEGF protein with 5 peptidic ligands is studied. Experiments were conducted in solution (NMR CSP, ITC) and in gas phase (CID TOF MS). Each ligand differs from others in chirality of one amino acid. It was shown, that trend of stability of the studied noncovalent complexes is reversed in the gas phase relatively to the solution. An explanation of this behavior is presented.

An inadequate transfer of oxygen can lead to rapid changes in cell physiology, metabolism, and cell death. In this work, the KLa is evaluated in a Taylor vortex flow bioreactor and a mass transfer correlation is proposed. The experimental conditions employed were: rotational Reynolds number between 244 and 2928, airflow rates varying from 80 mL.min-1 to 550 mL.min-1 and used culture medium DMEM.

In this work, a pressurized bioreactor was used for Y. lipolytica batch cultivation under increased air pressure from 1 bar to 6 bar. Cell growth was strongly enhanced by the pressure rise. The increase of oxygen availability caused the induction of the antioxidant enzyme SOD. The pre-growth of Y. lipolytica under increased pressure conditions did not affect the lipase production ability of the cells.

Pichia pastoris processes for heterolougous protein expression are typically run in three phases: a batch phase, an exponential feeding fed-batch phase and a long oxygen transfer limitation (OTL) phase. The final protein titre is often limited by the oxygen availability in the OTL phase. Here a direct adaptive controller is designed for the OTL phase. The controller was validated in pilot P. pastoris cultivations expressing a single chain antibody fragment (ScFv). This work shows that the propos

The combination of flow chemistry and microwave heating offers a unique opportunity to scale out heterogeneous microwave-assisted organic reactions. The feasibility of this setup is depending on the presence of a microwave effect, safety issues and energy efficiency.