05 Nov 2012 - 09 Nov 2012 - Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
Deadline for applications: 6 July 2012
This joint EBI-Wellcome Trust workshop provides hands-on training in the basics of mass spectrometry and proteomics bioinformatics, search engines, quantitative approaches including SRM/MRM, MS data repositories, the use of public databases for protein analysis, annotation of subsequent protein lists and incorporation of information from molecular interaction and pathway databases. The workshop is aimed at research scientists with a minimum of a degree in a biological discipline, including laboratory and clinical staff as well as specialists in related fields. The practical elements of the course will take raw data from a proteomics experiment and analyse it. Participants will be able to go from MS spectra, identify peptides and finally to lists of protein identifiers that can be analysed further using a wide range of resources. The final aim is to provide attendees with the practical bioinformatics knowledge they need to go back to the lab and process their own data (including large datasets) when collected.
Topics and Resources covered include:
Mass Spectroscopy Basics
MS Search Engines
Sequence databases and their use
Proteomics data and services integration
Standardising proteomics data
MS proteomics repositories
PRIDE - The Proteomics Identification Database, PRIDE Converter and PRIDE Inspector
Protein interaction data through IntAct
Using ontologies to annotate data
Placing proteins in pathways using Reactome
Protein sequence data in UniProt and InterPro
The course will be taught by trainers from the European Bioinformatics Institute and will include distinguished guest speakers.