Thermo Fisher Scientific has announced its new Thermo Scientific GlycoLink Immobilization and Immunoprecipitation (IP) Kits for protein affinity purification and the study of protein interactions.
The GlycoLink™ Immobilization and IP Kits provide the reagents for coupling glycosylated biological molecules to a beaded resin.
The kits contain the Thermo Scientific UltraLink Hydrazide Resin and a reaction catalyst for a fast and efficient method to immobilize carbohydrates and glycoproteins such as antibodies onto a durable polyacrylamide resin.
The catalyzed reaction results in greater than 90 percent coupling in four hours or less, depending on the amount of glycosylation.
It also leaves other critical active sites on the molecule such as amines, carboxyls and sulfhydryls free for other experimental purposes.
Several kit configurations are available and contain the complete set of buffers, oxidizing reagent and resin. The GlycoLink Coupling Catalyst is also available separately.