Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>News>This Article

Breakthrough Proteomics Technique SWATH™ Acquisition is Made Available to the Worldwide Scientific Community

Published: Wednesday, April 18, 2012
Last Updated: Wednesday, April 18, 2012
Bookmark and Share
Data-independent SWATH™ Acquisition on the TripleTOF™ 5600 system enables comprehensive proteome-wide quantitation and is published in Molecular and Cellular Proteomics.

Scientists at ETH Zurich, along with AB SCIEX have completed their development of MS/MSALL with SWATH™ Acquisition, a mass spectrometry-based proteomics technique that quantifies nearly all peptides and proteins in a sample from a single analysis. The method was published this year in Molecular and Cellular Proteomics.

Liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) is widely used by proteomics researchers, but current methods do not provide the speed, quantitative accuracy, specificity, sensitivity and reproducibility that are needed to produce complete and quantitative proteome maps of biological systems.

For the first time, SWATH™ Acquisition uses a data-independent MS/MS acquisition method to systematically generate complete, high-specificity fragment ion maps that can be queried for the presence and quantity of any protein of interest using a targeted data analysis strategy.

The new method depends on an advanced hybrid quadrupole-time of flight mass spectrometer, the AB SCIEX TripleTOF™ 5600 System.

The unique combination of high resolution, mass accuracy and ultra-high MS/MS spectral acquisition rates mean it is the only mass spectrometer on which the method can be performed efficiently.

SWATH™ Acquisition with the TripleTOF 5600 System, an extension of the AB SCIEX MS/MSALL functionality, will soon be commercially available and has been developed as part of a collaboration between AB SCIEX and Dr. Ruedi Aebersold and his team at ETH Zurich.

“SWATH™ Acquisition allows us to explore multiplexed MS/MS datasets to a level that was not possible with traditional clustering or database approaches,” said Ruedi Aebersold, Ph.D., Professor, Institute of Molecular Systems Biology at the Swiss Federal Institute of Technology (ETH Zurich).

Aebersold continued, “By collaborating with AB SCIEX to develop the technique on the TripleTOF™ 5600, we have made this breakthrough method available for proteomics researchers to conduct comprehensive quantitation on the entire proteome.”

The technique generates highly specific fragment ion data for all precursors within the monitored range.

Unlike traditional acquisition approaches, this technique does not rely on precursor ion mass detection to trigger MS/MS acquisition.

Instead, it systematically fragments all components of a sample through a rapidly moving selection window.

From the resulting data, several high resolution fragment ion chromatograms for each peptide of interest can be extracted to collectively identify the targeted peptide, as in selected or multiple reaction monitoring (SRM/MRM).

Working with ion libraries from public sources such as MRMAtlas, or generated in-house through database search software such as ProteinPilot™ Software, researchers can interrogate the dataset over and over again for quantitative data on peptides/proteins of interest.

The technique provides a complete qualitative and quantitative archive of the sample and can be retrospectively interrogated in silico as new hypotheses are developed.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

SCIEX and New Objective Partner
Partnership will provide advanced nanospray ionization technologies for next-generation proteomics.
Tuesday, May 26, 2015
SCIEX Announces OneOmics™ Collaborators
Advaita Bioinformatics, Researchers at Yale University and ISB launch applications and libraries that combine next-generation proteomics and next-generation sequencing data in innovative ways.
Thursday, March 19, 2015
AB SCIEX Wins New Product Innovation Award in Asia Pacific
The award recognizes the company’s continued innovation in the mass spectrometry industry and a number of new products introduced into the market in recent years.
Thursday, October 30, 2014
AB SCIEX Scientist Wins HUPO 2014 Science and Technology Award
Winner becomes second team member to be recognized for the commercialization of transformative chemistries such as iTRAQ® eagents.
Monday, October 06, 2014
AB SCIEX, Illumina Collaborate to Create Multi-omics Cloud-Computing Environment
Integration of SWATH™ Apps with BaseSpace® brings next-generation proteomics and next-generation sequencing together for the first time.
Thursday, October 02, 2014
Barry Karger Receives Arnold O. Beckman Award
Award honors Prof Karger for his outstanding achievements in the field of electrodriven separation techniques.
Monday, April 28, 2014
Researchers Solve a Mystery about Type 2 Diabetes Drug
AB SCIEX TripleTOF® and QTRAP® technologies support breakthrough medical study.
Friday, November 22, 2013
AB SCIEX Expands Support in the Middle East
AB SCIEX announces the opening of a new regional office and technical support centre located in Dubai.
Tuesday, October 29, 2013
AB SCIEX Receives Frost & Sullivan Award
AB SCIEX has been awarded the 2013 Global Market Share Leadership Award for Mass Spectrometry from Frost & Sullivan.
Thursday, September 12, 2013
AB SCIEX Proteomics Scientist Wins HUPO 2013 Science and Technology Award
Dr. Christie Hunter is being recognized for her contributions to the development and commercialization of a breakthrough approach for targeted proteomics.
Tuesday, September 10, 2013
AB Sciex to Combine Eksigent Chromatography with Capillary Electrophoresis from Beckman
The move brings together Beckman Coulter’s expertise in microscale separation with the expertise in microfluidics of the Eksigent chromatography team of AB Sciex.
Thursday, July 11, 2013
Institute for Systems Biology and AB SCIEX Partner
Collaboration with National Medal of Science award winner to accelerate development of new analytical strategies for proteomics mass spectrometry.
Friday, February 08, 2013
AB Sciex Agrees for Sigma-Aldrich® to Distribute iChemistry™ Solutions
Broadens availability of mass spectrometry-based tagging chemistries to biologists and chemists worldwide.
Wednesday, September 12, 2012
AB SCIEX Announces Academic Partnership Program
The program aims to increase the number of scientists who benefit from the company’s assistance and resources for advancing research in proteomics, metabolomics and lipidomics.
Wednesday, April 04, 2012
AB SCIEX Announces Academic Partnership Program to Support Biological Research
Focus on proteomics, metabolomics and lipidomics to accelerate promising work in science and biomedical studies.
Wednesday, March 28, 2012
Scientific News
Key to Natural Detoxifier’s Reactivity Discovered
Results have implications for health, drug design and chemical synthesis.
New Protein Found in Immune Cells
Immunobiologists from the University of Freiburg discover Kidins220/ARMS in B cells and demonstrate its functions.
Cell's Waste Disposal System Regulates Body Clock Proteins
New way to identify interacting proteins could identify potential drug targets.
How a Molecular Motor Untangles Protein
Diseases such as Alzheimer’s, Parkinson’s and prion diseases, all involve “tangled” proteins.
Compound Doubles Up On Cancer Detection
Researchers have found that tagging a pair of markers found almost exclusively on a common brain cancer yields a cancer signal that is both more obvious and more specific to cancer.
How Cell Growth Triggers Cell Division
Researchers in Jan Skotheim's lab have discovered a previously unknown mechanism that controls how large cells grow, an insight that could one day provide insight into attacking diseases such as cancer.
Probing the Forces Involved in Creating The Mitotic Spindle
Scientists at The Rockefeller University reveal new insights into the mechanical forces that govern elements of the mitotic spindle formation.
Identifying Cancer’s Food Sensors May Help to Halt Tumour Growth
Oxford University researchers have identified a protein used by tumours to help them detect food supplies. Initial studies show that targeting the protein could restrict cancerous cells’ ability to grow.
Specific Variations in RNA Splicing Linked to Breast Cancer
Researchers have identified cellular changes that may play a role in converting normal breast cells into tumors. Targeting these changes could potentially lead to therapies for some forms of breast cancer.
Thousands of Protein Interactions Identified
Thanks to the work by Utrecht University researcher Fan Liu and her colleagues, it is now possible to map the interactions between proteins in human cells.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos