Corporate Banner
Satellite Banner
Proteomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Observing the Binding Site and Dynamics of Protein S1 on a Bacterial Ribosome

Published: Monday, October 08, 2012
Last Updated: Monday, October 08, 2012
Bookmark and Share
Through the application of protein cross-linking and high resolution mass spectrometry, researchers from Indiana University have detailed the ribosomal binding site of S1 and have observed evidence of its dynamics.

Abstract
Ribosomal protein S1 has been shown to be a significant effector of prokaryotic translation. The protein is in fact capable of efficiently initiating translation, regardless of the presence of a Shine-Dalgarno sequence in mRNA. Structural insights into this process have remained elusive, as S1 is recalcitrant to traditional techniques of structural analysis, such as x-ray crystallography. Through the application of protein cross-linking and high resolution mass spectrometry, we have detailed the ribosomal binding site of S1 and have observed evidence of its dynamics. Our results support a previous hypothesis that S1 acts as the mRNA catching arm of the prokaryotic ribosome. We also demonstrate that in solution the major domains of the 30S subunit are remarkably flexible, capable of moving 30-50 Angstrom with respect to one another.

The article is published online in the journal Molecular & Cellular Proteomics and is free to access. 


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Proteomics for Systems Toxicology
MS-based proteomics is maturing into a robust technology for the measurement of proteome-wide exposure effects.
Monday, November 24, 2014
Identification of Psoriatic Arthritis Mediators in Synovial Fluid
This study represents the most comprehensive proteomic analysis of psoriatic arthritis synovial fluid, to date.
Friday, August 08, 2014
The Role of Mass Spectrometry in the "Omics" Era
The tremendous developments of the MS technology and concurrent gene sequencing efforts have made the “-omic” revolution possible.
Tuesday, February 18, 2014
Predictors of Obesity: The "Power" of the Omics
Diet and nutrient biomarkers should provide objective measures of dietary intake and nutritional status, as well as an integrated measure of intake, absorption and metabolism.
Friday, October 25, 2013
Label-free MS Proteome Quantification of Human Embryonic Kidney Cells Following 24 Hours of Sialic Acid Overproduction
Researchers have identified 1802 distinct proteins of which 105 proteins showed reliable expression changes.
Tuesday, August 13, 2013
Solid-Phase Glycan Isolation for Glycomics Analysis
In this article researchers from John Hopkins University reviewed current glycomics technologies for glycan purification, isolation, separation, and detection.
Thursday, June 27, 2013
A Nano LC-MALDI Mass Spectrometry Droplet Interface for the Analysis of Complex Protein Samples
In this article researchers from ETH Zürich have demonstrated a novel droplet-based interface between Nano–liquid chromatography and matrix assisted laser desorption ionisation mass spectrometry.
Thursday, June 06, 2013
Whole Gel Processing Procedure for GeLC-MS/MS Based Proteomics
The procedure will enable clinical proteomics for which GeLC-MS/MS is a popular workflow and sample numbers are relatively high.
Tuesday, May 14, 2013
Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Tissue Culture
Rapid development of MS-based proteomic techniques in the last decade that has immensely helped researchers in candidate biomarker discovery.
Monday, May 13, 2013
MultiAlign: A Multiple LC-MS Analysis Tool for Targeted Omics Analysis
This article demonstrates how two dissimilar LC-MS feature maps could be analyzed using MultiAlign’s MS/MS traceback and spectral clustering capability.
Tuesday, April 30, 2013
Identification of Plasma Lipid Biomarkers for Prostate Cancer by Lipidomics and Bioinformatics
This study explores a new approach to seeking biomarkers for prostate cancer and other diseases.
Monday, April 08, 2013
The HUPO Proteomics Standards Initiative- Mass Spectrometry Controlled Vocabulary
In this article, researchers describe the overall structure of the controlled vocabularies, the process by which it has been developed and is maintained and the dependencies on other ontologies.
Monday, March 25, 2013
Development and Characterization of a Novel Plug and Play LC-MS Source
This article reports the development and characterization of a novel, vendor neutral, ultra-high pressure compatible (~10,000 psi) LC-MS source.
Tuesday, March 12, 2013
'Omics' Approaches to Understanding Interstitial Cystitis/Painful Bladder Syndrome/Bladder Pain Syndrome
This review describes the current state of 'omics' studies and available data sets relevant to IC/PBS/BPS.
Monday, March 11, 2013
Optimal Precursor Ion Selection for LC-MALDI MS/MS
Researchers present two algorithms for inclusion list creation that formulate precursor ion selection as an optimization problem.
Tuesday, February 26, 2013
Scientific News
Cellular Contamination Pathway for Heavy Elements Identified
Berkeley Lab scientists find that an iron-binding protein can transport actinides into cells.
Lemon Juice and Human Norovirus
Citric acid may prevent the highly contagious norovirus from infecting humans, scientists discovered from the German Cancer Research Center.
Signature of Microbiomes Linked to Schizophrenia
Studying microbiomes in throat may help identify causes and treatments of brain disorder.
Structural Discoveries Could Aid in Better Drug Design
Scientists have uncovered the structural details of how some proteins interact to turn two different signals into a single integrated output.
Protein Found to Play a Key Role in Blocking Pathogen Survival
Calprotectin fends off microbial invaders by limiting access to iron, an important nutrient.
Study Identifies the Off Switch for Biofilm Formation
New discovery could help prevent the formation of infectious bacterial films on hospital equipment.
How DNA ‘Proofreader’ Proteins Pick and Edit Their Reading Material
Researchers from North Carolina State University and the University of North Carolina at Chapel Hill have discovered how two important proofreader proteins know where to look for errors during DNA replication and how they work together to signal the body’s repair mechanism.
Protein Found to Control Inflammatory Response
A new Northwestern Medicine study shows that a protein called POP1 prevents severe inflammation and, potentially, diseases caused by excessive inflammatory responses.
X-ray Laser Experiment Could Help in Designing Drugs for Brain Disorders
Scientists found that when two protein structures in the brain join up, they act as an amplifier for a slight increase in calcium concentration, triggering a gunshot-like release of neurotransmitters from one neuron to another.
Team Identifies Structure of Tumor-Suppressing Protein
An international group of researchers led by Carnegie Mellon University physicists Mathias Lösche and Frank Heinrich have established the structure of an important tumor suppressing protein, PTEN.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!