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Malvern’s Complementary Detector Arrays Boost GPC/SEC Productivity

Published: Friday, November 23, 2012
Last Updated: Friday, November 23, 2012
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Apply both dynamic and static light scattering for size and molecular weight measurement.

Malvern Instruments’ detector range for gel permeation/size exclusion chromatography (GPC/SEC) now includes the Zetasizer µV, a versatile size and molecular weight detector that can be added to any system to boost experimental productivity by maximizing information flow from a single experiment.

Complementary to Malvern’s established Viscotek range, which includes detectors for concentration, intrinsic viscosity and absolute molecular weight measurement, the Zetasizer µV is a dual integrated light scattering system with particular value for protein characterization.

It is easily connected to an existing GPC/SEC system using standard chromatography tubing and results reporting and analysis are efficiently handled through Malvern’s powerful OmniSEC software.

With GPC/SEC the productivity of analysis is directly impacted by the number of detectors applied to the eluting, fractionated samples.

The Zetasizer µV uses dynamic light scattering to secure precise molecular size measurement while at the same time offering static light scattering for absolute molecular weight measurement.

In protein characterization these capabilities support the detailed study of aggregation and conjugation and the flexibility to use the detector in cuvette mode is an important added bonus, one that allows the characterization of discrete samples with just 2 µL of material.

The versatile Zetasizer µV can be added to any protein SEC system to bring absolute molecular size and molecular weight capability to the chromatography experiment, and avoid the need to calibrate the column.

In addition, the change over from chromatography detector to ultra-sensitive batch DLS takes just a few seconds, maximizing the overall productivity of the instrument.


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