Corporate Banner
Satellite Banner
Proteomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Imaging Facility adds Two Tools for Microscopy

Published: Monday, February 18, 2013
Last Updated: Monday, February 18, 2013
Bookmark and Share
Cornell's Imaging Facility owns microscopes, scanners and ultrasound units for revealing details that can't be seen with the naked eye.

Now the facility has added two more state-of-the-art machines, one to extract tiny samples for genetic analysis and another to image fast microscopic events.

The Imaging Facility, located in Weill Hall and in the College of Veterinary Medicine, specializes in microscopy, whole organism imaging and high-resolution micro-CT (computed tomography), for visualizing samples from cells and engineered tissues to fossils, tomatoes and sharks.

The facility now has a spinning disk confocal microscope that enables users to image and manipulate fluorescent specimens rapidly; and an instrument for laser capture microdissection, which allows researchers to isolate specific cells or tissues from a sample by slicing out particular regions with a laser.

Once cells or tissues are cut, the laser capture microdissection machine pops the new sample into a tube where its genomic or proteomic profile can be analyzed. Normally, a sample with many tissue types is ground up and genetically sequenced. Researchers must then piece together fragments of genetic code from the potpourri. This new method allows researchers to isolate specific cells or regions prior to genetic analysis.

Cornell has two other laser capture microdissection tools on campus. The new one in the Imaging Facility is part of the Biotechnology Resource Center (BRC), which provides Cornell researchers with an array of state-of-the-art instruments and services. Managed by Cornell's Institute for Biotechnology, whose director is Jocelyn Rose, professor of plant biology, the BRC comprises seven facility laboratories focused on genomics (DNA sequencing, genotyping and microarrays), epigenomics, proteomics and mass spectrometry, bio-IT, bioinformatics and computational biology, advanced technology assessment and imaging. These tools and services are available to the university community and to investigators at other institutions.

The laser capture microdissection unit provides another capability for genomic analysis that can be bridged with other BRC resources for every phase of an experiment, said research scientist Rebecca Williams, the Imaging Facility's director.

"A researcher can microdissect a sample and end up with a tube of cells. Then we can help them find the correct genomics core facility" to have that sample sequenced, and then put them in touch with a biostatistics technician who can help analyze the sample, she added.

By raising and lowering the field of view, the new spinning disk confocal microscope can take repeated shots that are stitched together to create 3-D images. It can shoot more than 100 frames per second, allowing researchers to record such quick processes as calcium signaling or other fast movements. It also allows researchers to use photoactivated proteins (that turn on or change colors when hit with light) or photobleached proteins (that turn off when hit with light), providing insights to how proteins move within cells.

"Spinning disk confocal microscopes have been around for maybe 10 years, but this system is fully loaded; it's a fancy system," said Williams. It is fitted with three cameras for 3-D imaging of various colors, a photomanipulation unit, and control of such environmental conditions as carbon dioxide and oxygen levels.

Along with image analysis and visualization, the facility has software to manage the amount of data generated from taking 100 images per second, for example.

"Our job is to train people in these technologies and make it as easy as possible to use them," Williams said. "We train 100 researchers a year to use these microscopes."


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Gene Prevents Buildup of Misfolded Cell Proteins
For the first time, Cornell researchers have demonstrated how a gene called SEL1L plays a critical role in clearing away misfolded proteins.
Friday, January 24, 2014
Shark, Human Proteins are Surprisingly Similar
Despite widespread fascination with sharks, the world’s oldest ocean predators have long been a genetic mystery.
Friday, December 06, 2013
Gene Thought to be Linked to Alzheimer's is Marker for Only Mild Impairment
Defying the widely held belief that a specific gene is the biggest risk factor for Alzheimer's disease, report says that people with that gene are more likely to develop mild cognitive impairment -- but not Alzheimer's.
Monday, February 18, 2013
Study Finds How Stressed-Out Cells Halt Protein Synthesis
Researchers also find protein-making can be resumed once stress has passed.
Wednesday, January 09, 2013
Protein Regulates Protein Folding in Cells During Stress
Researchers link protein known for cell mobility with protein folding during stress.
Thursday, January 03, 2013
Study: How Cells form 'Trash Bags' for Recycling Waste
A class of membrane-sculpting proteins create vesicles that carry old and damaged proteins from the surface of cellular compartments into internal recycling plants where the waste is degraded and components are reused.
Tuesday, October 23, 2012
Proteins Barge in to Turn Off Unneeded Genes and Save Energy
When they activate a gene, living cells have a system in reserve to turn it off.
Friday, September 07, 2012
Cell Membrane Proteins Feel Long-Range Forces
Proteins embedded in the lipid membranes of cells feel long-range attractive forces in specific patterns that mediate the proteins' behaviour.
Wednesday, September 05, 2012
New Method Helps Researchers Decode Genomes
Although scientists sequenced the entire human genome more than 10 years ago, much work remains to understand what proteins all those genes code for.
Wednesday, September 05, 2012
Cell Membrane Proteins Feel Long-Range Forces
A team from Cornell have identified the physical mechanisms behind protein interactions, which are set off by changes in cellular membranes.
Thursday, August 30, 2012
Insights into Protein Folding May Lead to Better Flu Vaccine
New method for looking at how proteins fold allows researchers to take snapshots of ribosomes.
Friday, August 03, 2012
Bacteria Employ 'Quality-control' Machinery, say Biomolecular Engineers
Like quality-control managers in factories, bacteria possess built-in machinery that track the shape and quality of proteins trying to pass through their cytoplasmic membranes.
Friday, August 03, 2012
Scientific News
Cellular Contamination Pathway for Heavy Elements Identified
Berkeley Lab scientists find that an iron-binding protein can transport actinides into cells.
Lemon Juice and Human Norovirus
Citric acid may prevent the highly contagious norovirus from infecting humans, scientists discovered from the German Cancer Research Center.
Signature of Microbiomes Linked to Schizophrenia
Studying microbiomes in throat may help identify causes and treatments of brain disorder.
Structural Discoveries Could Aid in Better Drug Design
Scientists have uncovered the structural details of how some proteins interact to turn two different signals into a single integrated output.
Protein Found to Play a Key Role in Blocking Pathogen Survival
Calprotectin fends off microbial invaders by limiting access to iron, an important nutrient.
Study Identifies the Off Switch for Biofilm Formation
New discovery could help prevent the formation of infectious bacterial films on hospital equipment.
How DNA ‘Proofreader’ Proteins Pick and Edit Their Reading Material
Researchers from North Carolina State University and the University of North Carolina at Chapel Hill have discovered how two important proofreader proteins know where to look for errors during DNA replication and how they work together to signal the body’s repair mechanism.
Protein Found to Control Inflammatory Response
A new Northwestern Medicine study shows that a protein called POP1 prevents severe inflammation and, potentially, diseases caused by excessive inflammatory responses.
X-ray Laser Experiment Could Help in Designing Drugs for Brain Disorders
Scientists found that when two protein structures in the brain join up, they act as an amplifier for a slight increase in calcium concentration, triggering a gunshot-like release of neurotransmitters from one neuron to another.
Team Identifies Structure of Tumor-Suppressing Protein
An international group of researchers led by Carnegie Mellon University physicists Mathias Lösche and Frank Heinrich have established the structure of an important tumor suppressing protein, PTEN.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!