Corporate Banner
Satellite Banner
Proteomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Method Development to Estimate the Purity of Vesicle Preparations

Published: Thursday, February 28, 2013
Last Updated: Wednesday, February 27, 2013
Bookmark and Share
Nanoparticle tracking analysis is used to estimate the purity of vesicle preparations at the Cardiff University School of Medicine.

NanoSight reports on how Nanoparticle Tracking Analysis, NTA, is being used in the development of a method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration.

This work is reported by Dr Aled Clayton of the School of Medicine at Cardiff University in an original research article published in the Journal of Extracellular Vesicles.

The School of Medicine at Cardiff University is one of the largest medical schools in the United Kingdom, home to over 3000 students and staff.

It is a major international center for teaching and research providing a vibrant community of medical endeavor.

The School is based at the University Hospital of Wales in Cardiff, but is also embedded at other hospital sites in Cardiff including Velindre Cancer Centre.

Dr Aled Clayton and his team are researching the roles of exosomes in modulating tumor immunity and the cancer microenvironment in prostate cancer.

Exosomes are nanometer sized vesicles, produced in abundance by malignant cells but much of their physiological functions are incompletely understood.

Among Dr Clayton's interests are exosomes as possible disease markers, and has been using the NanoSight nanoparticle characterization system as a reliable tool to help develop projects in this field through the analysis of urine/serum from patients. He is keen to develop fluorescent methods with this platform in the future.

Describing his work, Dr Clayton says "as well as our own interests in cancer, we also collaborate with local colleagues interested in diverse conditions (Alzheimer's, cardiac disease, renal disease and others) in which vesicles may play a role. NanoSight's nanoparticle tracking analysis system has been a versatile platform aiding all of these studies."

Continuing, he said "prior to using NTA, there were few other options available. We had made use of electron microscopy but, we have come to rely on NTA as it is a very sensitive and versatile platform, well-suited to daily use."

The work of Dr Clayton and his postdoc, Dr Jason Webber, has recently been published in the Journal of Extracellular Vesicles where they posed the question "How pure are your vesicles?".

In this paper, they proposed a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nanovesicle counts to protein concentration, using tools including the NanoSight NTA platform and a colorimetric protein assay such as the BCA-assay.

They show this approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step.

The paper also proposes that the approach may aid in comparing/standardizing vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More than 4,900+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

NanoSight Announce New Appointment
Leading UK scientist, Dr Andrew Shuttleworth, joins NanoSight board as Director of Diagnostic Sciences.
Tuesday, June 26, 2012
Landmark Publication Reports Potential of Exosomes as Biomarkers for Early Disease Detection
NanoSight reports the new publication in one of the most cited peer reviewed journals in nanoscience and nanotechnology, NanoMedicine.
Thursday, July 07, 2011
NanoSight announces interactive webinar on January 27th on Exosome Characterization by Nanoparticle Tracking Analysis
NanoSight, world-leading manufacturers of unique nanoparticle characterization technology, will host an interactive webinar on the subject of exosome characterization by Nanoparticle Tracking Analysis.
Thursday, January 20, 2011
Scientific News
Oxygen Can Impair Cancer Immunotherapy
Researchers have identified a mechanism within the lungs where anticancer immune resposnse is inhibited.
Symmetry is Key to Collagen
Researchers describe how symmetry may be the key to growing collagen fibres outside the body.
Breakthrough in GPCR Understanding
Integral Molecular announces breakthrough in understanding the functionality of GPCRs, the largest class of drug targets in human disease.
Designing Ultrasound Tools with Lego-Like Proteins
Study outlines how ultrasound technology can be used for imaging in conjuction with protein engineering.
Enzyme that Triggers Cell Demise in ALS Identified
Scientists from Harvard have identified a key instigator of nerve cell damage in people with amyotrophic lateral sclerosis (ALS).
Molecular Alarm Clock Wakes Resting Ovules
Study of fruit flies yields discovery of a molecular "alarm clock" that activates resting ovules.
Catching Proteins in the Act
Scientists can now observe light activated processes in proteins through the use of free-electron x-ray lasers.
Proteins Preserve Vital Genetic Data
Research has shown how two key proteins bring about the oragnization of chromosomes and our genome.
Signaling Molecule Regulates Release of the Hunger Hormone Ghrelin
Researchers at UT Southwestern have identified that the blocking release of the hormone ghrelin may mediate low blood sugar effect in children taking beta blockers.
Telomere Replenishment in Real Time
Researchers have visualised the process of telomere attachment to chromosomes through single-molecule imaging.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!