Corporate Banner
Satellite Banner
Proteomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

NIH Study Uncovers Details of Early Stages in Muscle Formation and Regeneration

Published: Tuesday, April 23, 2013
Last Updated: Monday, April 22, 2013
Bookmark and Share
Mouse study findings may offer clues for understanding cell fusion.

Researchers at the National Institutes of Health have identified proteins that allow muscle cells in mice to form from the fusion of the early stage cells that give rise to the muscle cells.

The findings have implications for understanding how to repair and rehabilitate muscle tissue and to understanding other processes involving cell fusion, such as when a sperm fertilizes an egg, when viruses infect cells, or when specialized cells called osteoclasts dissolve and assimilate bone tissue in order to repair and maintain bones.

Their findings were published online in the Journal of Cell Biology.

"Through a process that starts with these progenitor cells, the body forms tissue that accounts for about one-third of its total weight," said the study's senior author, Leonid V. Chernomordik, Ph.D., of the Section on Membrane Biology at the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), the NIH institute where the research was conducted. "Our study provides the first look at the very early stages of this fusion process."

Dr. Chernomordik conducted the study along with researchers at NICHD (Evgenia. Leikina, D.V.M.,; Kamram. Melikov, Ph.D.; Samristha Sanyal, Ph.D.; Santosh Verma, Bokke Eun, Ph.D.; Claudia Gebert, D.V.M., Ph.D.; Karl Pfeifer, Ph.D., and Vladimir.A. Lizunov, Ph.D.) and at Tel Aviv University, in Israel (Michael M. Kozlov, Ph.D.).

Muscle cells originate from precursor cells known as myoblasts. Myoblasts fuse to form a single long tubular cell called a myocyte (a muscle fiber). Muscle tissue is composed of large collections of these fibers.

The fusion of myoblasts into muscle fibers takes place early in fetal development. With exercise and throughout a person's life, the process is repeated to form new muscle mass and repair old or damaged muscle.

It takes many hours for cells to prepare for fusion, but the fusion process itself is very rapid. To study myoblast fusion, the researchers first blocked the start of the fusion process with a chemical.

Ordinarily, the mouse myoblasts the researchers worked with fuse at varied intervals. By blocking fusion, and then lifting the block, the researchers were able to synchronize fusion in a large number of cells, making the process easier to study.

The researchers identified the two distinct stages of cell fusion and the essential proteins that facilitate these stages.

In the first stage, two myoblasts meet, and proteins on cell surface membranes cause the membranes to meld.

In the second stage, a pore opens between the cells and their contents merge. This second step is guided by proteins inside the cells.

The work identifies two cell surface proteins that act at the start of myoblast fusion. These proteins belong to a large family of proteins called annexins. Annexins are also known to play a role in membrane repair and in inflammation.

The researchers identified the protein dynamin, found inside the cell, as essential to the second stage of the cell fusion process.

"Dynamin also has an unexplained link to certain rare and poorly understood myopathies - disorders characterized by underdeveloped muscles," said Dr. Chernomordik.

Dr. Chernomordik continued, "We hope that further examination of the role of dynamin in cell fusion will lead to a greater understanding of these conditions."


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,000+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Detecting Bacterial Infections in Newborns
Researchers tested an alternative way to diagnose bacterial infections in infants—by analyzing RNA biosignatures from a small blood sample.
Wednesday, September 14, 2016
$12.4M Awarded to Neural Regeneration Projects
The National Institutes of Health will fund six projects to identify biological factors that influence neural regeneration.
Friday, September 02, 2016
Oxygen Can Impair Cancer Immunotherapy
Researchers have identified a mechanism within the lungs where anticancer immune resposnse is inhibited.
Friday, August 26, 2016
New Inflammatory Disease Discovered
NIH researchers have discovered a rare and potentially deadly disease - otulipenia - the mostly affects children.
Tuesday, August 23, 2016
How Parkinson’s Disease Alters Brain Activity Over Time
The NIH study provides a new tool for testing experimental medications aimed at alleviating symptoms and slowing the rate at which the diseases damage the brain.
Tuesday, August 16, 2016
Genetic Cause of Rare Pediatric Neuropathy Identified
NIH mouse study identifies the mechanism responsible for a rare form of pediatric neuropathy.
Thursday, August 04, 2016
Uncovering Rhinovirus C Structure
Researchers have determined the structure of rhinovirus C. Their findings may aid the development of antiviral therapies and vaccines.
Wednesday, July 27, 2016
Advancing Protein Visualization
Cryo-EM methods can determine structures of small proteins bound to potential drug candidates.
Friday, May 27, 2016
Study Finds Factors That May Influence Influenza Vaccine Effectiveness
Researchers at NIH have suggested that the long-held approach to predicting seasonal influenza vaccine effectiveness may need to be revisited.
Wednesday, April 20, 2016
Visualizing a Cancer Drug Target at Atomic Resolution
Using cryo-electron microscopy, researchers were able to view, in atomic detail, the binding of a potential small molecule drug to a key protein in cancer cells.
Wednesday, February 10, 2016
Genomic Signature Shared by Five Types of Cancer
National Institutes of Health researchers have identified a striking signature in tumor DNA that occurs in five different types of cancer.
Monday, February 08, 2016
Natural Protein Points to New Inflammation Treatment
Findings may offer insight to effective treatments for inflammatory diseases, such as rheumatoid arthritis, psoriasis, and multiple sclerosis.
Friday, February 05, 2016
Biomarkers Outperform Symptoms in Parsing Psychosis Subgroups
Multiple biological pathways lead to similar symptoms - NIH-funded study.
Thursday, December 10, 2015
NIH Supports New Studies to Find Alzheimer’s Biomarkers in Down Syndrome
Initiative will track dementia onset, progress in Down syndrome volunteers.
Tuesday, December 01, 2015
Dementia Linked to Deficient DNA Repair
Mutant forms of breast cancer factor 1 (BRCA1) are associated with breast and ovarian cancers but according to new findings, in the brain the normal BRCA1 gene product may also be linked to Alzheimer’s disease.
Tuesday, December 01, 2015
Scientific News
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
One Step Closer to Precision Medicine for Chronic Lung Disease Sufferers
A study led by University of North Carolina at Chapel Hill, and National Jewish Health, has provided evidence of links between SNPs and known COPD blood protein biomarkers.
New Therapeutic Target for Crohn’s Disease
A promising new target for drugs that treat IBD has been identified along with a possible biomarker for IBD severity.
Uncovering Water Bear Resilience
A protein identified in water bears can protect DNA of human cells from lethal doses of radiation damage.
Potential of New Insect Control Traits in Agriculture
Researchers have discovered a protein that shows promise as an alternate corn rootworm control mechanism.
Peer Reviewed Study Demonstrates Mass Spec Technique
The peer reviewed study demonstrates MS workflow, TMTCalibrator workflow, which dramatically enhances detection of key early stage Alzheimer’s biomarkers.
Enhancing Antibiotics to Defeat Resistant Bacteria
Scientists enhance ability of antibiotics to defeat resistant types of bacteria using molecules called PPMOs
Disordered Protein 'Shape Shifts' to Avoid Crowding
Study suggests disordered protein escapes from the cell membrane when it runs out of space.
Hyperstable Peptides for 'On-Demand' Drugs
These small molecules can fold into different conformations that could allow for greater flexibility in precision drug design
Antibodies Block Norovirus’ Entrance into Cells
Scientists have uncovered a mechanism in the human body that targets and successfully blocks noroviruses.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!