Corporate Banner
Satellite Banner
Proteomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Roche Launches cOmplete His-Tag Purification Column

Published: Thursday, June 27, 2013
Last Updated: Thursday, June 27, 2013
Bookmark and Share
Free choice of purification conditions and enhanced protein stabilization in a pre-packed format.

Roche has announced the launch of its new cOmplete His-Tag Purification Column for researchers performing histidine-tagged protein purification from lysates.

The new column uses Roche’s proprietary nickel-chelate chemistry and is compatible with commonly used reducing agents (DTT), metalloproteinase inhibiting reagents such as EDTA, and different buffer and salt environments.

This allows researchers to choose optimal buffer conditions for the target protein in a convenient pre-packed format.

“We have received very positive feedback from pilot users who appreciate the flexibility of being able to purify proteins under protein protecting conditions,” said Ruedi Stoffel, Head of Biochemical Reagents & Custom Biotech.

Stoffel continued, “It enables our customers to apply any buffer required to obtain proteins of high purity, stability and function.”

Following the purification step, the new column does not require buffer exchange or resin recharging therefore avoiding a costly and time consuming process.

In addition, the resin’s minimized nickel ion leakage not only reduces toxic nickel waste, but also stabilizes the target protein by preventing nickel ions from catalyzing protein oxidation.

Thanks to the additional format, researchers can choose their preferred method of purification. The cOmplete His-Tag Purification Resin can be used for batch purification as well as on automated systems based on FPLC.

Pre-packed cOmplete His-Tag Purification Columns are available in two sizes (1 ml and 5 ml) and are compatible with standard instruments for protein purification.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More than 4,900+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Roche Invests in Stratos Genomics
Investment by Roche will advance Stratos Genomics chemistry for nanopore sequencing.
Friday, June 27, 2014
Roche's Troponin T Nominated for International Inventor Award
European Patent Office nominates the team of Klaus Hallermayer from Roche Penzberg, jointly with Prof. Hugo Katus from the University of Heidelberg.
Monday, June 11, 2012
Roche NimbleGen Commits to GMP and FDA Submission for Microarrays
Roche NimbleGen announces a company-wide initiative to implement Good Manufacturing Processes for its suite of DNA microarrays.
Thursday, April 08, 2010
imaGenes GmbH Joins Roche NimbleGen’s Certified Service Provider Program for ChIP-chip Analysis
imaGenes achieves status as a CSP of NimbleGen microarrays for Chromatin Immunoprecipitation.
Wednesday, December 16, 2009
Roche and the Department of Health of Junta de Andalucía Collaborate in Spanish Medical Genome Project
The research project aims to analyze the genomes of people with rare diseases and identify the defective gene or genes causing the disease and its behavior.
Tuesday, December 01, 2009
Inhibition of Cell Migration by Gene Silencing - Measured with the xCELLigence Cell Analysis System from Roche
Researchers used the RTCA DP Instrument with the CIM-Plate 16 to evaluate the influence of a cellular protein on the migration potential of a tumor.
Monday, November 30, 2009
FDA Grants Emergency Use Authorization to Roche’s Assay for 2009 H1N1 Influenza Virus
Roche’s RealTime ready Influenza A/H1N1 Detection Set for the 2009 H1N1 influenza virus has received emergency use authorization from the FDA.
Tuesday, November 17, 2009
Analysis of RNA Polymerase II Mutants using Roche’s xCELLigence System
In a recent research study, Roche’s xCELLigence RTCA SP Instrument was used for in vitro testing of cells to test entirely synthetic CTDs.
Friday, October 23, 2009
RiNA GmbH Acquires Roche RTS Protein Expression Portfolio
Both the companies have agreed to transfer the complete Roche Applied Science RTS Protein Expression portfolio to RiNA GmbH.
Wednesday, September 09, 2009
Roche and Google.org Start Initiative for Early Discovery of New Diseases
The joint project is aimed to demonstrate the feasibility of developing a multidisciplinary surveillance, research and response system.
Wednesday, July 29, 2009
Real-time and Dynamic Monitoring of Virus-mediated Cytopathogenicity with Roche’s xCELLigence System
Göttingen University study utilizes xCELLigence system from Roche Applied Science for testing of virus-mediated cytopathogenicity.
Friday, May 01, 2009
Genome Sequencer FLX System Installed at University of Barcelona
The Genome Sequencer FLX will be installed at the Genomics Unit of the Scientific and Technical Services at UB.
Friday, April 03, 2009
Next-Generation Sequencing Uncovers Genetic Variation Linking Type 1 Diabetes with Enterovirus Infection
Science study discovers rare mutations in a disease-associated antiviral response gene that reduce the risk developing type 1 diabetes.
Friday, March 06, 2009
Roche NimbleGen Microarrays Selected for the Neuromuscular Disorder -Chip Consortium
The NMD-Chip Consortium selects Roche NimbleGen microarrays to accelerate and simplify genetic research of neuromuscular disorders.
Friday, February 27, 2009
Scientific News
Oxygen Can Impair Cancer Immunotherapy
Researchers have identified a mechanism within the lungs where anticancer immune resposnse is inhibited.
Symmetry is Key to Collagen
Researchers describe how symmetry may be the key to growing collagen fibres outside the body.
Breakthrough in GPCR Understanding
Integral Molecular announces breakthrough in understanding the functionality of GPCRs, the largest class of drug targets in human disease.
Designing Ultrasound Tools with Lego-Like Proteins
Study outlines how ultrasound technology can be used for imaging in conjuction with protein engineering.
Enzyme that Triggers Cell Demise in ALS Identified
Scientists from Harvard have identified a key instigator of nerve cell damage in people with amyotrophic lateral sclerosis (ALS).
Molecular Alarm Clock Wakes Resting Ovules
Study of fruit flies yields discovery of a molecular "alarm clock" that activates resting ovules.
Catching Proteins in the Act
Scientists can now observe light activated processes in proteins through the use of free-electron x-ray lasers.
Proteins Preserve Vital Genetic Data
Research has shown how two key proteins bring about the oragnization of chromosomes and our genome.
Signaling Molecule Regulates Release of the Hunger Hormone Ghrelin
Researchers at UT Southwestern have identified that the blocking release of the hormone ghrelin may mediate low blood sugar effect in children taking beta blockers.
Telomere Replenishment in Real Time
Researchers have visualised the process of telomere attachment to chromosomes through single-molecule imaging.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!