Corporate Banner
Satellite Banner
Proteomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

New Stain-Free Gel Visualization Application of Syngene’s G:BOX System

Published: Monday, September 16, 2013
Last Updated: Monday, September 16, 2013
Bookmark and Share
Helps save time and make protein analysis safer.

Scientific Digital Imaging’s [SDI’s] Syngene Division has announced that the lighting and filter conditions in the G:BOX image analysis system have been optimized to allow faster, safer visualization of proteins on stain-free gels.

Technical specialists at Syngene tested filter and lighting conditions in a G:BOX XR5 image analysis system to accurately image a range of proteins (2-300ng) run on a Criterion TGX Stain-Free gel.

For optimum imaging performance, the gel was exposed to 5 minutes of mid wave UV (302nm) on a UV transilluminator and the gel was imaged using a UV filter and an exposure time of 2 seconds. The gel was then stained with ProtoBlue Safe and imaged again.

The G:BOX image analysis results from the stained and unstained gel showed the linearity and sensitivity of both methods of protein visualization was comparable with proteins being detected in the 2-20ng range.

In fact, with some proteins, the stain- free gels provided better detection limits than the ProtoBlue Safe dye staining.

Further details of this research can be found in Application Note 64 on the Syngene web site
(http://www.syngene.com/assets/doc/Technical-notes/Stainfree-gels-64.pdf).

Dr. Lindsey Kirby, SDI’s Syngene Application Specialist explained: “Staining protein gels can be a time consuming task as scientists often use Coomassie Blue, which is toxic and requires a lengthy de-staining step. Therefore, if scientists can image their gels immediately after they have run them, this makes analysis safer and more efficient.”

Lindsey added: “This is why we’ve worked to develop the optimal imaging conditions for stain-free visualization. We’re delighted that our G:BOX technology is capable of such excellent performance in this application because biologists regularly working with protein gels can now confidently use the G:BOX image analysis system for rapid stain-free visualization without compromising on sensitivity or safety.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,100+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Syngene Appoints VWR International as New Canadian Distributor
Companies to provide superior service and support for life scientists in Canada.
Thursday, March 28, 2013
G:BOX Chemi XT Analyser at Major Cancer Research Centre
Developed to detect DNA and proteins associated with tumour generation.
Wednesday, October 27, 2010
Major Molecular Medicine Centre Uses G:BOX iChemi XT Image Analyser
Syngene’s imaging system is used to identify proteins that impact the pathology of muscular dystrophy.
Monday, October 25, 2010
University of York uses G:BOX Imaging System
Syngene’s imaging being used as fast and cost-effective system of assessing protein expression.
Tuesday, June 01, 2010
Versatile Dyversity System at Leading European Cancer Research Centre
UK Cambridge Research Institute uses Dyversity to accelerate the pace of research on proteins and DNA associated with a variety of different cancers.
Friday, May 07, 2010
Versatile Dyversity System at Leading European Cancer Research Centre Rapidly Analyses Fluorescent and Chemiluminescent Proteins with Ease
Syngene, a world-leading manufacturer of image analysis sol- utions, is pleased to announce its Dyversity multi-application image analyser is being used by scientists in The Cancer Research UK Cambridge Research Institute (CRI) at the University of Cambridge, one of Europe’s major cancer research hubs, to accelerate the pace of research on proteins and DNA associated with a variety of different cancers.
Thursday, May 06, 2010
Einstein Researchers Uses Dyversity Image Analysis System for Proteins Detection
Syngene’s 2D gel imaging system helps to detect proteins associated with longer human life span.
Friday, June 19, 2009
G:BOX Chemiluminescence Imager is used at Edinburgh Napier University
Napier Researchers are using the G:BOX chemiluminescence imaging system to determine the pre-clinical potential of anti-cancer compounds.
Monday, May 11, 2009
2D Gel Imaging Study Comparing CCD Imager with Laser Scanner
Study shows CCD imager produces same image quality 10 times faster than the leading laser scanner.
Thursday, May 25, 2006
Syngene Announces new Fluorescence Application of G:BOX Chemi
Makes studying differential protein expression on one Western blot a reality.
Monday, April 24, 2006
Scientific News
Structure of Essential Digestive Enzyme Uncovered
Using a powerful combination of techniques from biophysics to mathematics, researchers have revealed new insights into the mechanism of a liver enzyme that is critical for human health.
Getting a Better Look at How HIV Infects and Takes Over its Host Cells
A new approach, developed by a team of researchers led by The Rockefeller University and The Aaron Diamond AIDS Research Center (ADARC), offers an unprecedented view of how a virus infects and appropriates a host cell, step by step.
Untangling Disease-Related Protein Misfolding
Work advances understanding of genetic forms of thrombosis, emphysema, cirrhosis of the liver, neurodegenerative diseases and inflammation, among others.
Scientists Find Evidence That Cancer Can Arise Changes
Researchers at Rockefeller University have found a mutation that affects the proteins that package DNA without changing the DNA itself can cause a rare form of cancer.
US-India Collab Finds Molecular Signatures of Severe Malaria
Study may be a significant advancement in understanding the causes of severe malaria.
Triple-Negative Breast Cancer Target Is Found
Researchers at UC Berkeley discover a target that drives cancer metabolism in triple-negative breast cancer.
Crucial Reaction for Vision Revealed
Scientists have tracked the reaction of a protein responding to light, paving the way for a new understanding of life's essential reactions.
Cancer Can Arise from Histone Mutations
A mutation that affects the proteins that package DNA—without changing the DNA itself—can cause a rare form of cancer.
Mimicking Evolution to Create Novel Proteins
A study by researchers in the Kuhlman lab offers a new route to design the 'cellular machines' needed to understand and battle diseases.
Can Gender Play A Role In Determining Cancer Treatment Choices?
MD Anderson study reveals “sex-biased” gene signatures in review of 13 cancer types.
Scroll Up
Scroll Down
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,100+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!