Corporate Banner
Satellite Banner
Proteomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Zetasizer APS Analyzes Sticky Proteins with No Cross-Contamination

Published: Monday, October 14, 2013
Last Updated: Sunday, October 13, 2013
Bookmark and Share
New application note published on Malvern Instruments website.

A new application note published on the Malvern Instruments website demonstrates the absence of any cross contamination when using the company’s Zetasizer APS (Auto Plate Sampler) to analyze known ‘sticky’ proteins.

The Zetasizer APS dynamic light scattering (DLS) system enables the automated measurement of multiple samples and has wide application in the rapid screening of proteins.

The risk of cross-contamination is a concern to many people when considering automated analysis in a multiwell format and the experiment was designed to thoroughly test the instrument’s capabilities. The application note can be downloaded at www.malvern.com/dlsautomation.

Setup and operation of the Zetasizer APS is straightforward and its stringent cleaning protocols are designed to ensure that no cross contamination occurs.

The work described in the new application note used familiar protein samples to demonstrate the lack of detectable cross-contamination.

Samples included insulin, in both “monomeric” (the insulin hexamer) and “oligomeric” forms, and Abeta, the amyloid beta peptide known for its stickiness and tendency to adhere to plastic. The Abeta sample was present in two forms, fibrillar and oligomeric.

The order in which samples were loaded onto the system was chosen so as to maximize the risk of cross-contamination.

The oligomeric insulin was dispensed first using a standard pipette, followed by the small and pure insulin, and then the larger polydisperse Abeta samples.

Since large protein aggregates scatter much more light than small pure protein samples, if cross-contamination were to occur the polydisperse Abeta sample would be detected within the small homogenous protein sample. The results show that after running multiple tests, there was no detectable cross contamination.

The Zetasizer APS delivers the same high sensitivity, high specification DLS measurements as other systems in Malvern’s established Zetasizer family.

The automated processing of samples in multiwell plates enables the generation of high quality DLS data with no user intervention, a major benefit in maximizing productivity.

The system’s highly practical data display capability allows easy retrieval of results of interest, eliminating the need to manually scan through all measurements. In addition, the Zetasizer APS plate navigator feature acts as a data mapping tool, for rapid screening of results to retrieve the information of most interest and subsequent in-depth investigation of selected data sets.

The separate temperature controls of the plate holder and the measurement cell allow the Zetasizer APS to maintain the protein samples in optimal condition until measurement. In addition, thermal trend measurements between 2°C to 90°C with 0.1°C degree precision can be defined.

The plate scheduler allows the user to graphically set up size and thermal trend measurements of various samples from the same plate using a variety of different SOPs.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Malvern Signs Exclusive Agreement with Affinity Biosensors
Agreement to distribute Affinity Biosensors’ Archimedes system extends Malvern Instruments’ biopharma solutions.
Tuesday, March 05, 2013
Malvern Specialist Discusses Light Scattering Techniques at BioProcess International Europe 2011
Dr Hanna Jankevics will present a poster at the 7th annual BioProcess International Europe conference in Nice (France).
Thursday, April 14, 2011
Malvern Specialist Tests Sensitivity Limits of Dynamic Light Scattering
Malvern’s Zetasizer Nano systems use light scattering techniques to measure the hydrodynamic size, zeta potential and molecular weight of proteins and nanoparticles.
Wednesday, October 06, 2010
Scientific News
HIV Particles Used to Trap Intact Mammalian Protein Complexes
Belgian scientists from VIB and UGent developed Virotrap, a viral particle sorting approach for purifying protein complexes under native conditions.
Potential “Good Fat” Biomarker
New method to measure the activity of energy consuming brown fat cells could ease the testing weight loss drugs.
Computational Model Finds New Protein-Protein Interactions
Researchers at University of Pittsburgh have discovered 500 new protein-protein interactions (PPIs) associated with genes linked to schizophrenia.
New Insights into Gene Regulation
Researchers have solved the three-dimensional structure of a gene repression complex that is known to play a role in cancer.
Controlling RNA in Living Cells
Modular, programmable proteins can be used to track or manipulate gene expression.
Soy Shows Promise as Natural Anti-Microbial Agent
Soy isoflavones and peptides may inhibit the growth of microbial pathogens that cause food-borne illnesses, according to a new study from University of Guelph researchers.
Potential Target for Revolutionary Antibiotics
An international team of including the Lomonosov Moscow State University researchers discovered which enzyme enables Escherichia coli bacterium (E. coli) to breathe.
DNA Barcodes Gone Wild
A team of researchers at University of Toronto’s Donnelly Centre and Sinai Health System’s Lunenfeld-Tanenbaum Research Institute (LTRI) has developed a new technology that can stitch together DNA barcodes inside a cell to simultaneously search amongst millions of protein pairs for protein interactions.
Biomarkers for Profiling Prostate Cancer Patients
Exiqon A/S has announced the publication of validation of prognostic microRNA biomarkers for the aggressiveness of prostate cancer in independent cohorts.
Grant to Fund Million Peaks Project
The European Research Council (ERC) has awarded a prestigious Advanced Grant to Prof. Peter Schoenmakers, Prof. Albert Polman and Prof. Huib Bakker, all three of whom work at the University of Amsterdam (UvA).
Scroll Up
Scroll Down
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!