Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>News>This Article

Straightforward Protein Characterization at the NKI

Published: Wednesday, November 06, 2013
Last Updated: Wednesday, November 06, 2013
Bookmark and Share
Avacta Analytical’s Optim® technology for the study of novel proteins.

Researchers at the Netherlands Cancer Institute (Nederlands Kanker Instituut, NKI) are benefitting from the advanced, multi-parameter characterization offered by Avacta Analytical’s Optim® technology for the study of novel proteins.

Dr Anastassis Perrakis, Principle Investigator in the Institute’s Structural Biology Group, explained: “We are interested in the mechanisms controlling a variety of different cellular processes, using in vitro protein characterization to look at how proteins interact with other proteins and DNA. In addition to traditional biochemical assays, we perform a large number of biophysical experiments to help us understand these molecular interactions, and first became interested in the Optim instrument when we were having trouble measuring the melting curve for a particular protein of interest.”

The Optim instrument allows the Group to probe multiple protein stability-indicating parameters at high speed, using ultra-low sample volumes of proteins and other molecules.

“We have had the instrument since May, and it has been very useful,” Anastassis continued. “It looks directly at the unfolding process through the physical properties of the protein - you do not need to use a reporter system - so the results are more precisely defined. The melting point data offers us a good indication of the quality of the proteins we express, as well as ensuring that they are stable and ‘happy’ under the conditions we plan to use in our biochemical assays, without excessive denaturation or aggregation. The biggest advantage for us is that the Optim instrument uses between 10 and 50 times less sample than competitive techniques, which is obviously a real benefit for downstream studies.”

Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,200+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Avacta Sign Third Distribution Agreement with Pall Life Sciences
Deal marks Avacta’s entry to the third largest Asia-Pacific biotech market.
Friday, September 28, 2012
Avacta Analytical Partners with UCL
Avacta Analytical is partnering with University College London (UCL) as an industrial sponsor of the EPSRC Centre for Innovative Manufacturing in Emergent Macromolecular Therapies.
Friday, November 18, 2011
Scientific News
Integrated Omics Analysis
Studying multi-omics promises to give a more holistic picture of the organism and its place in its ecosystem, however despite the complexities involved those within the field are optimistic.
RNA-Binding Proteins Role in ALS Revealed
Researchers describe how damage to RNA-binding protein contributes to ALS, isolating a possible therapeutic target.
Advances in Alzheimer’s Research
Researchers show how a diseased vertebrate brain can naturally react to Alzheimer’s pathology by forming more neurons.
Study Finds Key Regulator in Pulmonary Fibrosis
Researchers identify an enzyme that could open the way to therpies for chronic fatal lung disease.
NIH Study Determines Key Differences between Allergic and Non-Allergic Dust Mite Proteins
Researchers at NIH have uncovered factors that lead to the development of dust mite allergy and assist in the design of better allergy therapies.
Alzheimer’s-Linked Protein May Play Role in Schizophrenia
Researchers suggests a protein linked to cognitive decline in Alzheimer's also plays a role in genetic predisposition to schizophrenia.
Peptides vs. Superbugs
Scientists successfully develop a shuttle system made of liquid-crystalline nanomaterials that protect peptites.
Immune Approach Targets Humans Instead of Bacteria
Scientists show for the first time how bacterial superantigen toxins work, and how short peptides can block them and save lives.
Cocoa Compound Linked to Some Cardiovascular Biomarker Improvements
The study highlights the urgent need for large, long-term RCTs that improve understanding of how the short-term benefits of cocoa flavanol intake on cardiometabolic biomarkers may be translated into clinical outcomes.
Could 2D Mass Spec Breakthrough Lead to Medical Revolution?
Pharmaceutical research could be quicker and more precise, thanks to an innovative breakthrough in 2D mass spec from the University of Warwick.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,200+ scientific videos