Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>News>This Article

Diamond Light Source Purchases Multiple Electron Microscopes from FEI

Published: Thursday, June 19, 2014
Last Updated: Thursday, June 19, 2014
Bookmark and Share
The U.K.’s national synchrotron now has a complete cryo-EM workflow for integrative structural biology to explore essential biological questions at the atomic and molecular scale.

FEI has announced its largest order for Life Sciences - Diamond Light Source, one of the most advanced synchrotron light sources in the world, has ordered two Titan Krios™ cryo transmission electron microscopes (TEMs), a Scios™ DualBeam™ FIB/SEM (focused ion beam/scanning electron microscope) and a Talos™ cryo-TEM.

These microscopes form the core of the electron biology facility (EBIC) that will provide Diamond with a complete cryo-electron microscopy (EM) workflow that will be used in conjunction with other structural biology techniques to enable new insights into viruses and cellular proteins.

Professor David Stuart, director for Life Sciences at Diamond Light Source, states, “X-ray diffraction (XRD) and nuclear magnetic resonance (NMR) are extremely powerful techniques that can resolve atomic-scale structure, but can only be applied to a subset of biological molecules and complexes. Cryo-EM can resolve structures down to the sub-nanometer, molecular-scale, and can look at just about anything, including large multimolecular complexes. In the simplest sense, integrative structural biology uses cryo-EM to provide the overview, and XRD and NMR to see the details.”

He adds, “Locating cryo-EM equipment at the synchrotron gives researchers access to a range of advanced capabilities at the same facility. The new Cryo EM centre for biology is being funded by a £15.6 million grant from the Wellcome Trust, the Medical Research Council (MRC) and the Biotechnology and Biological Sciences Research Council (BBSRC).”

“When the new Cryo-EM centre opens in 2015, the UK’s national synchrotron will house the essential tools needed to carry out a comprehensive and integrated structural biology approach for characterization of viruses and protein complexes,” states Dr. Paul Scagnetti, vice president of FEI’s Science Business Group. “Answers to many of the most important biological questions, from basic biological functions to complicated disease processes, can potentially be discovered by understanding the structure and function of the molecular machines that operate in this spatial regime.”

Integrative structural biology is a rapidly-emerging field that combines general methods including TEM. For this process Diamond’s sophisticated computational capabilities are critical for analyzing the data from individual techniques and combining these results to obtain 3D structural analysis that spans the spatial scale from atoms to large multimolecular entities.

One of the Krios instruments will be dedicated to single particle analysis (SPA), which can resolve structural details down to a few tenths of a nanometer - small enough to identify individual side chains on the amino acid building blocks of proteins.

The other Krios cryo-TEM will be optimized for cellular tomography, which can look at naturally-occurring configurations of molecules in selected regions of whole cells. The Scios DualBeam and Talos cryo-TEM will be part of the sample preparation workflow.

Scagnetti adds, “Diamond Light Source combines an advanced synchrotron with broad and deep technical expertise. Professor David Stuart, who played a leading role in the cryo EM acquisition, is one of the best-known experts in the field of XRD and structural biology. We are delighted to be working with him and to see the fast growing acceptance of cryo-EM into the discipline of integrative structural biology.”

The instruments will be located at Diamond Light Source on the Harwell Science and Innovation Campus near Oxford, United Kingdom. Diamond, a third-generation synchrotron light source, generates high-intensity beams at frequencies that range from microwaves to hard X-rays, providing opportunities for scientific research in a number of disciplines.

The systems will be installed in Q1 2015.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Tsinghua University Selects FEI’s Titan Krios Cryo-Electron Microscope
Investment in Titan Krios underscores Tsinghua University’s commitment to leadership in structural biology.
Wednesday, August 26, 2009
UCLA’s New Center for NanoBiology Begins Sub-Nanometer Molecular Imaging with FEI Titan Krios Microscope
FEI’s automated molecular imaging solution will be used to understand the causes of disease.
Friday, August 07, 2009
Scientific News
Resurrected Proteins Double Their Natural Activity
Researchers demonstrate method for reviving denatured proteins.
Scientists Decode Structure at Root of Muscular Disease
Researchers at Rice University and Baylor College of Medicine have unlocked the structural details of a protein seen as key to treating a neuromuscular disease.
Sniffing Out Cancer
Scientists have been exploring new ways to “smell” signs of cancer by analyzing what’s in patients’ breath.
A New Single-Molecule Tool to Observe Enzymes at Work
A team of scientists at the University of Washington and the biotechnology company Illumina have created an innovative tool to directly detect the delicate, single-molecule interactions between DNA and enzymatic proteins.
Milestone Single-Biomolecule Imaging Technique May Advance Drug Design
The first nanometer resolved image of individual tobacco mosaic virions shows the potential of low-energy electron holography for imaging biomolecules at a single particle level; a milestone in structural biology and a potential new tool for drug design.
Researchers Discover A New Mechanism of Proteins to Block HIV
Certain IFITM proteins block and inhibit cell-to-cell transmission of HIV.
A Natural Light Switch
MIT scientists identify and map the protein behind a light-sensing mechanism.
Biologists Find Unexpected Role for Amyloid-Forming Protein
Yeast protein could offer clues to how Alzheimer’s plaques form in the brain.
Study Adds to Evidence That Viruses Are Alive
A new analysis supports the hypothesis that viruses are living entities that share a long evolutionary history with cells, researchers report.
How Flu Viruses Gain The Ability To Spread
New study reveals the soft palate is a key site for evolution of airborne transmissibility.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos