Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>News>This Article

Diamond Light Source Purchases Multiple Electron Microscopes from FEI

Published: Thursday, June 19, 2014
Last Updated: Thursday, June 19, 2014
Bookmark and Share
The U.K.’s national synchrotron now has a complete cryo-EM workflow for integrative structural biology to explore essential biological questions at the atomic and molecular scale.

FEI has announced its largest order for Life Sciences - Diamond Light Source, one of the most advanced synchrotron light sources in the world, has ordered two Titan Krios™ cryo transmission electron microscopes (TEMs), a Scios™ DualBeam™ FIB/SEM (focused ion beam/scanning electron microscope) and a Talos™ cryo-TEM.

These microscopes form the core of the electron biology facility (EBIC) that will provide Diamond with a complete cryo-electron microscopy (EM) workflow that will be used in conjunction with other structural biology techniques to enable new insights into viruses and cellular proteins.

Professor David Stuart, director for Life Sciences at Diamond Light Source, states, “X-ray diffraction (XRD) and nuclear magnetic resonance (NMR) are extremely powerful techniques that can resolve atomic-scale structure, but can only be applied to a subset of biological molecules and complexes. Cryo-EM can resolve structures down to the sub-nanometer, molecular-scale, and can look at just about anything, including large multimolecular complexes. In the simplest sense, integrative structural biology uses cryo-EM to provide the overview, and XRD and NMR to see the details.”

He adds, “Locating cryo-EM equipment at the synchrotron gives researchers access to a range of advanced capabilities at the same facility. The new Cryo EM centre for biology is being funded by a £15.6 million grant from the Wellcome Trust, the Medical Research Council (MRC) and the Biotechnology and Biological Sciences Research Council (BBSRC).”

“When the new Cryo-EM centre opens in 2015, the UK’s national synchrotron will house the essential tools needed to carry out a comprehensive and integrated structural biology approach for characterization of viruses and protein complexes,” states Dr. Paul Scagnetti, vice president of FEI’s Science Business Group. “Answers to many of the most important biological questions, from basic biological functions to complicated disease processes, can potentially be discovered by understanding the structure and function of the molecular machines that operate in this spatial regime.”

Integrative structural biology is a rapidly-emerging field that combines general methods including TEM. For this process Diamond’s sophisticated computational capabilities are critical for analyzing the data from individual techniques and combining these results to obtain 3D structural analysis that spans the spatial scale from atoms to large multimolecular entities.

One of the Krios instruments will be dedicated to single particle analysis (SPA), which can resolve structural details down to a few tenths of a nanometer - small enough to identify individual side chains on the amino acid building blocks of proteins.

The other Krios cryo-TEM will be optimized for cellular tomography, which can look at naturally-occurring configurations of molecules in selected regions of whole cells. The Scios DualBeam and Talos cryo-TEM will be part of the sample preparation workflow.

Scagnetti adds, “Diamond Light Source combines an advanced synchrotron with broad and deep technical expertise. Professor David Stuart, who played a leading role in the cryo EM acquisition, is one of the best-known experts in the field of XRD and structural biology. We are delighted to be working with him and to see the fast growing acceptance of cryo-EM into the discipline of integrative structural biology.”

The instruments will be located at Diamond Light Source on the Harwell Science and Innovation Campus near Oxford, United Kingdom. Diamond, a third-generation synchrotron light source, generates high-intensity beams at frequencies that range from microwaves to hard X-rays, providing opportunities for scientific research in a number of disciplines.

The systems will be installed in Q1 2015.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Tsinghua University Selects FEI’s Titan Krios Cryo-Electron Microscope
Investment in Titan Krios underscores Tsinghua University’s commitment to leadership in structural biology.
Wednesday, August 26, 2009
UCLA’s New Center for NanoBiology Begins Sub-Nanometer Molecular Imaging with FEI Titan Krios Microscope
FEI’s automated molecular imaging solution will be used to understand the causes of disease.
Friday, August 07, 2009
Scientific News
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Gut Microbes Signal to the Brain When They're Full
Don't have room for dessert? The bacteria in your gut may be telling you something.
Turning up the Tap on Microbes Leads to Better Protein Patenting
Mining millions of proteins could become faster and easier with a new technique that may also transform the enzyme-catalyst industry, according to University of California, Davis, researchers.
Exploring the Causes of Cancer
Queen's research to understand the regulation of a cell surface protein involved in cancer.
Measuring microRNAs in Blood to Speed Cancer Detection
A simple, ultrasensitive microRNA sensor holds promise for the design of new diagnostic strategies and, potentially, for the prognosis and treatment of pancreatic and other cancers.
Novel Proteins Linked to Huntington's Disease
University of Florida Health researchers have made a new discovery about Huntington's disease, showing that the gene that causes the fatal disorder makes an unexpected "cocktail" of mutant proteins that accumulate in the brain.
Enzyme Critical to Maintaining Telomere Length Discovered
New method expected to speed understanding of short telomere diseases and cancer.
New Method Identifies Up to Twice as Many Proteins and Peptides
An international team of researchers developed a method that identifies up to twice as many proteins and peptides in mass spectrometry data than conventional approaches.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos