|Functional Analysis of RAD51B and RAD23B SNPs using Circular Chromosome Conformation Capture (4C) in Human Prostate Cell Lines|
C. Cieza-Borrella1, S. Wakerell1 , Q. Karlsson1 , R.A. Eeles1,2, Z. Kote-Jarai1
The aim of this study is to understand the mechanism behind the association between single nucleotide polymorphisms (SNPs), lying in non-coding regions near or within RAD51B and RAD23B, and prostate cancer risk by analysing their interactions with the whole genome using Circular Chromosome Conformation Capture (4C).
|CRISPR-Cas9 genome editing utilizing chemically synthesized RNA|
Michael Delaney, Kaizhang He, Eldon Chou, Amanda Haas, Žaklina Strezoska, Melissa L. Kelley, and Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA
Chemical synthesis has been easily applied for rapidly generating either crRNA and tracrRNA or synthetic sgRNA for direct delivery into cells for gene editing applications such as DNA-free options and high throughput arrayed screening.
|The Chemical Synthesis of Long and Highly Modified RNA using 2'-ACE Chemistry|
Xiaoqin Cheng, Kristina Larson, Letitia Kwok, David Mierzejewski, Shawn Begay, Randy Rauen, Kelly Grimsley, Kaizhang He, Michael Delaney, David Kitchen, Amanda Haas, Melissa Kelley, Anja van Brabant Smith
Dharmacon has previously developed a novel RNA synthesis chemistry making RNA synthesis as reliable, accessible and of comparable quality as routinely observed in DNA synthesis.
|Blood Transcriptomic Diagnosis of Active TB with Five Genes|
JK Roe1*, N Thomas1, E Gil1, K Best1, E Tsaliki1, S Morris-Jones2, S Stafford1, N Simpson1, KD Witt3, B Chain1, R Miller4, A Martineau3, M Noursadeghi1
This study aimed to identify the minimal set of genes that can accurately diagnose active TB, both pulmonary and extrapulmonary, and evaluate their specificity.
|Experimental design considerations for efficient and specific gene knockin using a CRISPR-Cas9 for HDR with synthetic crRNA and tracrRNA|
Hidevaldo B. Machado, John A. Schiel, Maren Mayer-Gross, Eldon T. Chou, Melissa L. Kelley, Anja van Brabant Smith. Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA
Precise genome engineering with CRISPR-Cas9 and single-stranded DNA oligo or double-stranded DNA plasmid donors via homology-directed repair (HDR).
|Exosomes promote survival in squamous head and neck cancer cells after ionizing radiation|
Lisa Mutschelknaus, Carsten Peters, Klaudia Winkler, Ramesh Yentrapalli, Theresa Heider, Michael J. Atkinson, Simone Moertl
Exosomes are important intercellular communicators in the acute radiation stress-response. These nanometer-sized extracellular vesicles confer protective signals to neighboring cells and therefore promote the tumorigenic and radioresistant phenotype of head and neck cancer cells.
|Super-resolution single molecule localization microscopy of the exocytotic machinery underlying insulin secretion.|
Deirdre M. Kavanagh, Alison Dun, Rory R. Duncan, and Colin Rickman.
Single molecule imaging of the tSNARE proteins involved in insulin secretion.
|Resistance-Associated Variants in HCV Genotype 1 Populations|
Elian Rakhmanaliev1, Pramila Ariyaratne1, Zhang Rui1, Kok Siong Poon2, Cui Wen Chua2, Mui Joo Khoo2, Evelyn S. Koay2, Ekawat Passomsub3, Wasun Chantratita3, Gerd Michel1
In this study we specifically investigated RAVs emerging in HCV genotype 1.
|Integrated Next-Generation Sequencing and qPCR Workflow in Clinical Diagnostics|
Elian Rakhmanaliev, Tatiana Ivanova, Gerd Michel
Combined automated qPCR and NGS Sentosa workflow is a reliable and efficient in vitro diagnostics (IVD) tool for the detection and/or quantitation of a wide range of bacterial and viral pathogens as well as gene mutations.
|A Synthetic CRISPR-Cas9 System for Homology-directed Repair|
John A. Schiel, Maren M. Gross, Emily M. Anderson*, Eldon T. Chou, Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA
Synthetic, dual-RNA-encoded Cas9 is used for precise homology-directed repair (HDR) gene engineering. Both short and long (GFP) inserts are covered.
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