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LC-MS/MS METHOD FOR THE QUANTIFICATION OF PITOFENONE IN HUMAN PLASMA
B.M. Gurupadayya, S. Sharfuddin and R.S. Chandan

We developed LCMS/MS bioanalytical method for quantitative estimation of Pitofenone HCl in plasma is simple, sensitive, accurate, precise, and selective. Percentage recovery shows that the method is free from interference of matrix. The analytical method presented here has proved to be useful for investigation of the characteristics of Pitofenone in human plasma in pharmacokinetic and pharmacogenetic studies.

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Experimental Evolution in Diatoms - Thermal longterm acclimation in two model diatom species
Katrin Schmidt1, Sinéad Collins2, Thomas Mock1

Experimental Evolution in two model Diatoms

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Antioxidant and Free Radicals Scavenging Activity of Biomolecules: Hydroxamic Acids
Bharati Verma and Rama Pande

In the present poster attention is focused towards the investigation of antioxidant and free radicals scavenging activity of N-aryl substituted hydroxamic acids by DPPH method which is based on the reduction of alcoholic DPPH solutions in presence of a hydrogen donating antioxidant, hydroxamic acids.

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"In-vitro studies on hydroxamic acid- CT DNA binding"
Rubi Khilari

In vitro studies of five the interaction between five different derivatives of hydroxamic acids, N-phenyl 2,4dichloro phenoxybutyro, N-m-tolyl 2,4 dichlorophenoxyglutero, N-m-tolyl-4-chlorophenoxyaceto, N-m-chloro-phenyl-tertiarybutylbenzo and N-p-tolyl-iso-valero hydroxamic acids and calf thymus DNA was investigated under simulated physiological condition.

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Efficacy of Gene Therapy in Restoring Vision in Leber's Congenital Amaurosis
Elizabeth O'Donnell

The research reviews current knowledge of the genetic causes of Leber's congenital amaurosis (LCA) and the development of a gene therapy intervention for LCA. The efficacy as well as the limitations of this form of treatment are evaluated in detail and recommendations are made for the future directions of gene therapy for Leber's congenital amaurosis.

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METHOD DEVELOPMENT FOR THE SIMULTANEOUS DETERMINATION OF METHYLMERCURY AND INORGANIC MERCURY IN SEAFOOD
Sergio Carneado, Ariane V. Zmozinski, Carmen Ibáñez-Palomino, Àngels Sahuquillo, José Fermín López-Sánchez, Márcia M. da Silva

This work reports the method development for the simultaneous determination of methylmercury (MeHg+) and inorganic mercury (iHg) species in seafood. The study focused on the extraction and quantification of MeHg+ by liquid chromatography coupled to on-line UV irradiation and cold vapour atomic fluorescence spectroscopy (LC-UV-HG-AFS), using HCl 4 mol L-1 as extractant agent.

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Microextraction techniques combined with X-ray fluorescence spectrometry for determination of different metal ions in water
Karina Kocot (a), Beata Zawisza (a), Eva Marguí (b), Ignasi Queralt (c), Rafal Sitko (a)

In present work new preconcentration procedures combining microextraction techniques and X-ray fluorescence spectroscopy were developed. Proposed methodologies are promising tools for multielemental analysis enabling detection of natural pollutants in different water samples.

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Recent Technical Developments in the Standardized Separation and Measurement of Extracellular Vesicles
Amy Phillips (1), Robert Vogel(2), and Murray F. Broom(1)

We present robust and practical solutions for the standardization of EV isolation and concentration measurement. EV concentration also needs to have a defined size range. With these solutions, significant improvement in the reproducibility and comparability of results between different researchers is possible.

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High Titre BacMam viruses improve transduction efficiency of mammalian cells
Robert Possee, Elisabetta Locanto, Adam Chambers, Linda King

We present a method for producing high titre BacMam virus vectors for the efficient transduction of mammalian cells.

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Novel Approaches to High-throughput Measurements of Replicative Lifespan in Yeast by Microfluidic Size Sorting and Genetic Engineering
Ilka Lewrenz, Stephanie Wälter, Jacqueline Franke

Development of a high-throughput screen utilizing a microfluidic device and a linearly growing yeast strain to simultaneously test the influence of various drugs on the replicative lifespan of yeast.

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Showing Results 1 - 10 of 186
Scientific News
Non-Gluten Proteins as Targets of Immune Response to Wheat in Celiac Disease
The results were reported online in the Journal of Proteome Research.
Biomarker Discovery Sheds New Light on Heart Attack Risk of Arthritis Drugs
Drug may be given a new lease of life.
Metal Test Could Help Diagnose Breast Cancer Early
It may be possible to develop a simple blood test that, by detecting changes in the zinc in our bodies, could help to diagnose breast cancer early.
Mouse Study Reveals Potential Clue to Extra Fingers or Toes
NIH-funded study finds that gene appears to regulate protein signals inside the cell.
Two Studies Identify A Detectable, Pre-Cancerous State In The Blood
Findings pave way for new lines of cancer research focused on detection and prevention.
Proteomics for Systems Toxicology
MS-based proteomics is maturing into a robust technology for the measurement of proteome-wide exposure effects.
Schizophrenia May be Triggered by Excess Protein
Neuroscientist Bonnie Firestein says too much causes abnormalities in brain development.
Protein that Rouses the Brain from Sleep May be Target for Alzheimer's Prevention
Scientists at Washington University have established links between sleep problems and Alzheimer’s.
Protein-engineered Cages Aid Studies of Cell Functions
The Cages, from researchers at Tokyo Institute of Technology, to deliver an important signalling molecule, carbon monoxide, into cells.
UCLA Biochemists Build Largest Synthetic Molecular ‘Cage’ Ever
New nanoscale protein container could lead to synthetic vaccines and offer a way to deliver medicine inside of human cells.
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