|Antioxidant and Free Radicals Scavenging Activity of Biomolecules: Hydroxamic Acids|
Bharati Verma and Rama Pande
In the present poster attention is focused towards the investigation of antioxidant and free radicals scavenging activity of N-aryl substituted hydroxamic acids by DPPH method which is based on the reduction of alcoholic DPPH solutions in presence of a hydrogen donating antioxidant, hydroxamic acids.
|"In-vitro studies on hydroxamic acid- CT DNA binding" |
In vitro studies of five the interaction between five different derivatives of hydroxamic acids, N-phenyl 2,4dichloro phenoxybutyro, N-m-tolyl 2,4 dichlorophenoxyglutero, N-m-tolyl-4-chlorophenoxyaceto, N-m-chloro-phenyl-tertiarybutylbenzo and N-p-tolyl-iso-valero hydroxamic acids and calf thymus DNA was investigated under simulated physiological condition.
|Efficacy of Gene Therapy in Restoring Vision in Leber's Congenital Amaurosis|
The research reviews current knowledge of the genetic causes of Leber's congenital amaurosis (LCA) and the development of a gene therapy intervention for LCA. The efficacy as well as the limitations of this form of treatment are evaluated in detail and recommendations are made for the future directions of gene therapy for Leber's congenital amaurosis.
|METHOD DEVELOPMENT FOR THE SIMULTANEOUS DETERMINATION OF METHYLMERCURY AND INORGANIC MERCURY IN SEAFOOD|
Sergio Carneado, Ariane V. Zmozinski, Carmen Ibáñez-Palomino, Àngels Sahuquillo, José Fermín López-Sánchez, Márcia M. da Silva
This work reports the method development for the simultaneous determination of methylmercury (MeHg+) and inorganic mercury (iHg) species in seafood. The study focused on the extraction and quantification of MeHg+ by liquid chromatography coupled to on-line UV irradiation and cold vapour atomic fluorescence spectroscopy (LC-UV-HG-AFS), using HCl 4 mol L-1 as extractant agent.
|Microextraction techniques combined with X-ray fluorescence spectrometry for determination of different metal ions in water|
Karina Kocot (a), Beata Zawisza (a), Eva Marguí (b), Ignasi Queralt (c), Rafal Sitko (a)
In present work new preconcentration procedures combining microextraction techniques and X-ray fluorescence spectroscopy were developed. Proposed methodologies are promising tools for multielemental analysis enabling detection of natural pollutants in different water samples.
|Recent Technical Developments in the Standardized Separation and Measurement of Extracellular Vesicles|
Amy Phillips (1), Robert Vogel(2), and Murray F. Broom(1)
We present robust and practical solutions for the standardization of EV isolation and concentration measurement. EV concentration also needs to have a defined size range. With these solutions, significant improvement in the reproducibility and comparability of results between different researchers is possible.
|Quantitative Real-time PCR differentiation between Genetically Modified pollen and Genetically Modified flour in honey|
Eugénia de Andrade1*, Maria Lopes2, Maria Clara Fernandes1, Fátima Quedas2, Isabel Rodrigues1, Amélia Maria Lopes1
We investigated the ability of quantitative real-time PCR, using plasmids calibrants, together with the triploid maize seed endosperm and the haploid pollen, to get good estimates for the copy number of a transgene in relation to the copy number of a species specific gene as a mean to distinguish between pollen and flour from maize.
|High Titre BacMam viruses improve transduction efficiency of mammalian cells|
Robert Possee, Elisabetta Locanto, Adam Chambers, Linda King
We present a method for producing high titre BacMam virus vectors for the efficient transduction of mammalian cells.
|Novel Approaches to High-throughput Measurements of Replicative Lifespan in Yeast by Microfluidic Size Sorting and Genetic Engineering|
Ilka Lewrenz, Stephanie Wälter, Jacqueline Franke
Development of a high-throughput screen utilizing a microfluidic device and a linearly growing yeast strain to simultaneously test the influence of various drugs on the replicative lifespan of yeast.