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Human iPSC-derived hepatocytes and cardiomyocytes for drug toxicity testing
AnnandRR; Vardaro R; Hamilton B; Akakira R; Tamura K; Yoshida S; Lin YC; Toyoda D; Kogami H; Okuda Y; Watanabe T; Inamura M

Human iPS-derived hepatocytes (ReproHepato™) and cardiomyocytes (ReproCardio 2™) are useful for in vitro toxicity assays.

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Improved Small RNA Library Preparation Workflow for Next-Generation Sequencing
Sabrina Shore, Jordana Henderson, Anton McCaffrey, Gerald Zon, Richard Hogrefe

We describe an optimized small RNA NGS library prep workflow using chemically modified adapters which suppresses adapter dimers, allows for RNA inputs down to 1 ng and eliminates the need for a gel purification step, thus allowing full automation not previously possible.

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Flexible automated platform for blood group genotyping on DNA microarrays
S. Paris1, D. Rigal1, V. Barlet1, M. Verdier1, N. Coudurier2, P. Bailly3, J.-C. Brès1,4

The purpose of this project was to set up and validate a flexible robotic platform using 96-well DNA microarray for multiplex blood group genotyping.

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Combined Use of Rheometry and GPC/SEC for Characterising Ionic Polysaccharides
John Duffy, Bernd Schaefer, Bert Postma

This poster shows how Rheometry and GPC can be complementary tools for characterising the behaviour of polysaccharides in solution

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Rate and Mechanistic Investigation of Eu(OTf)2-Mediated Reduction of Graphene Oxide at Room Temperature
Tufan Ghosh, Sandeepan Maity and Edamana Prasad

Eu(OTf)2 has been introduced as an efficient reagent for the reduction of graphene oxide. Details kinetic studies have been performed which suggests that, the method is more efficient compared to that of other commonly used reduction methods.

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Electrical Fusion Machine - New electrical fusion system for x-ray fluorescence analysis
Dr. Rainer Schramm, FLUXANA GmbH & Co. KG; Prof. Marie-Louise Klotz, Hochschule Rhein-Waal, Dr. Myint Myint Sein, Hochschule Rhein-Waal

This poster describes sample preparation in x-ray fluorescence analysis using the Electrical Fusion Machine by FLUXANA.

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Fluorescent Nanoprobes Confined in a Drop as a novel Sensing Platform for Detection of Metal Species at Trace Level
Carlos Bendicho, Isabel Costas-Mora, Vanesa Romero, Isela Lavilla

In the last years, a great interest has arisen concerning the design and development of new optical probes for the sensitive and selective detection of chemical species making use of miniaturized and environmental friendly methods. In this sense, quantum dots (QDs) and metal nanoclusters (NCs) have important optical properties to be applied in analytical systems as fluorescent probes.

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Specificity of highly potent miRNA inhibitors
Barbara Robertson, Andrew Dalby, Yuriy Fedorov, Jon Karpilow, Anastasia Khvorova1, Devin Leake, Annaleen Vermeulen

miRNA inhibitors are invaluable tools for elucidating the roles of miRNAs. However, potent inhibitors may also affect other miRNAs. To understand the potential cross-reactivity of miRNA inhibitors, various miRNA inhibitor designs were systematically tested. We demonstrate that mismatches both within and outside the seed region of the miRNA interfere with inhibition. Our findings indicate that features important for natural miRNA target recognition are also important for inhibitor specificity.

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Alternative miRNA design for therapeutic RNAi applications
Anja van Brabant Smith, Barb Robertson, Annaleen Vermeulen, Christina Yamada, Angela Reynolds, Anastasia Khvorova, Devin Leake

For in vivo applications, the design of miRNA inhibitors and miRNA mimics must be optimized for stability and potency. However, stabilized miRNA mimic molecules can lose functionality compared to standard miRNA mimic molecules due, in part, to the activity of the stabilized passenger strand acting as a miRNA inhibitor. We discuss how mismatches affect the activity of the stabilized miRNA mimics, perhaps by generating a passenger strand that is less functional as an inhibitor molecule.

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Showing Results 21 - 30 of 252
Scientific News
Advancing Cancer Drug Design with Image of Key Protein
Scientists have pioneered the use of a high-powered imaging technique to picture in exquisite detail one of the central proteins of life – a cellular recycling unit with a role in many diseases.
'Invisible' Protein Structure Explains the Power of Enzymes
A research group at Umeå University in Sweden has managed to capture and describe a protein structure that, until now, has been impossible to study.
Long-term Memories Are Maintained by Prion-like Proteins
Research from Eric Kandel’s lab at Columbia University Medical Center has uncovered evidence of a system in the brain that persistently maintains memories for long periods of time.
A New Path Towards a Universal Flu Vaccine
New research suggests it may be possible to harness a previously unknown mechanism within the immune system to create more effective and efficient vaccines against this ever-mutating virus.
Brain Cells Switch Epigenetic Gears Throughout Life
Research finds that histone turnover regulates how genes in the brain are turned on and off in response to various stimuli, thereby allowing neurons to form new synaptic connections.
Outsmarting HIV With Vaccine Antigens Made to Order
AIDS vaccine researchers may be one step closer to outwitting HIV, thanks to designer antibodies and antigens made to order at Duke University.
Animals’ Genomic Buffers May Help Humans
Researchers at Duke University School of Medicine and Brigham and Women’s Hospital, Harvard Medical School have identified a mechanism that explains why some mutations can be disease-causing in one genome but benign in another.
Protein Responsible for Blood Vessel Growth in Tumours Discovered
Scientists have discovered a new protein which triggers the growth of blood vessels in breast cancer tumours which have spread to the brain, a common location which breast cancer can spread to.
New Genetic Form of Obesity and Diabetes Discovered
Scientists have discovered a new inherited form of obesity and type 2 diabetes in humans.
Informatics Tool Helps Scientists Prioritize Protein Modification Research
Researchers have developed a new informatics technology that analyzes existing data repositories of protein modifications and 3D protein structures to help scientists identify and target research on "hotspots" most likely to be important for biological function.
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