|A mix-and-read cell-based assay for antibody screening against Epithelial Growth Factor Receptor |
Wayne P Bowen, David Onley, Paul Wylie, Diana Caracino and Tristan Cope
Here we present a sensitive robust, mix-and-read method for the screening of antibodies against cell surface proteins. With its simple operation, no-wash format, and high sensitivity, this new method is well-suited for high throughput antibody screening.
|Fast Liquid Differential Scanning Calorimetry (FLDSC)|
R. Splinter, A.W. van Herwaarden, A. Pfreundt, W.E. Svendsen, D. Istrate, W. van Eijk
Lysozyme experiments show that protein unfolding can be recorded at scan rates of up to 1000 °C/s, and for lysozyme concentrations of 1 % and probably even down to 0.1%.
|Protein Microarrays for Characterisation of the Bacillus anthracis ‘infectome’|
Stephen Kidd, Karen Kempsell, Rebecca Ingram, Pierre Watteau, Daniel Altmann, Michael Elmore, Sue Charlton, Bassam Hallis and Richard Vipond
These studies provide an interesting insight into the transcriptome of virulent B. anthracis in a host infection environment.
|Low cost, low footprint, expandable automated biobanking solutions |
James Craven, Chris Morris, Maud Godfrey, Danielle Miller
This case study describes how TTP Labtech’s comPOUND storage modules have been employed by Abcam, a worldwide supplier of antibodies. Here, the turnaround time of sample placement and retrieval is an essential component for high quality service to its customers.
|Monitoring Protein Synthesis in Living Cells With Fluorescent Labeled tRNA FRET Pairs|
Zeev Smilansky, Sima Barhoom, Ian Farrel, Dvir Dahary, Andrew Leask, Peter Vanderklish, Marcelo Ehrlich, Barry S. Cooperman and Orna Elroy-Stein
We transfect cells with tRNAs labeled as FRET donors and acceptors. A FRET signal is generated only when a donor labeled tRNA and an acceptor-labeled tRNA come in close contact (< 7 nM), as they do on the ribosome during the elongation cycle. The intensity of the FRET signal correlates with the number of ribosomes engaged in protein synthesis, providing a real-time, live-cell assay for measuring rates of protein synthesis.
|Determination and Comparison of Specifics of Nucleus Pulposus Cells of Human Intervertebral Disc in Alginate and Chitosan– Gelatin Scaffolds|
Masoud Ghorbani, Hamid Bahramian Renani , Batool Hashemibeni Beni, Zeinab Karimi
In this work we studied specefices of NP cells obtained from nucleus pulposus by collagenase enzymatic hydrolysis obtained from patients undergoing open surgery.
|Improved Ligation Specificity with Chemically Modified Ligation Components|
Sabrina Shore, Alexandre Lebedev, Elena Hidalgo Ashrafi, Gerald Zon, Natasha Paul, Richard Hogrefe
Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.
|Metal Polymers, A Glue to Immobilise Proteins Onto Synthetic Surfaces|
Abernethy N, Chung E, Fontanelle BT, Gao Y, Jennins D, Koudijs MM, Lim D, Yang L, Ling T, Vukovic P, Wong A, Maeji, NJ
The main objective of this work was to develop a surface chemistry which maintains protein function and orientation per unit surface area, regardless of the surface used.
|Identification of Novel BACE-1 Inhibitors through an Advanced Structure-Based Virtual Screening Protocol|
Puneet Kacker, Angela De Simone, Matteo Masetti, Giovanni Bottegoni, Vincenza Andrisano, Andrea Cavalli
This study assesses the influence of different ligand chemotypes on the protonation state of the catalytic dyad.