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Tuesday, September 02, 2014
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A mix-and-read cell-based assay for antibody screening against Epithelial Growth Factor Receptor
Wayne P Bowen, David Onley, Paul Wylie, Diana Caracino and Tristan Cope

Here we present a sensitive robust, mix-and-read method for the screening of antibodies against cell surface proteins. With its simple operation, no-wash format, and high sensitivity, this new method is well-suited for high throughput antibody screening.

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Fast Liquid Differential Scanning Calorimetry (FLDSC)
R. Splinter, A.W. van Herwaarden, A. Pfreundt, W.E. Svendsen, D. Istrate, W. van Eijk

Lysozyme experiments show that protein unfolding can be recorded at scan rates of up to 1000 °C/s, and for lysozyme concentrations of 1 % and probably even down to 0.1%.

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Protein Microarrays for Characterisation of the Bacillus anthracis ‘infectome’
Stephen Kidd, Karen Kempsell, Rebecca Ingram, Pierre Watteau, Daniel Altmann, Michael Elmore, Sue Charlton, Bassam Hallis and Richard Vipond

These studies provide an interesting insight into the transcriptome of virulent B. anthracis in a host infection environment.

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Low cost, low footprint, expandable automated biobanking solutions
James Craven, Chris Morris, Maud Godfrey, Danielle Miller

This case study describes how TTP Labtech’s comPOUND storage modules have been employed by Abcam, a worldwide supplier of antibodies. Here, the turnaround time of sample placement and retrieval is an essential component for high quality service to its customers.

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Monitoring Protein Synthesis in Living Cells With Fluorescent Labeled tRNA FRET Pairs
Zeev Smilansky, Sima Barhoom, Ian Farrel, Dvir Dahary, Andrew Leask, Peter Vanderklish, Marcelo Ehrlich, Barry S. Cooperman and Orna Elroy-Stein

We transfect cells with tRNAs labeled as FRET donors and acceptors. A FRET signal is generated only when a donor labeled tRNA and an acceptor-labeled tRNA come in close contact (< 7 nM), as they do on the ribosome during the elongation cycle. The intensity of the FRET signal correlates with the number of ribosomes engaged in protein synthesis, providing a real-time, live-cell assay for measuring rates of protein synthesis.

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Determination and Comparison of Specifics of Nucleus Pulposus Cells of Human Intervertebral Disc in Alginate and Chitosan– Gelatin Scaffolds
Masoud Ghorbani, Hamid Bahramian Renani , Batool Hashemibeni Beni, Zeinab Karimi

In this work we studied specefices of NP cells obtained from nucleus pulposus by collagenase enzymatic hydrolysis obtained from patients undergoing open surgery.

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Improved Ligation Specificity with Chemically Modified Ligation Components
Sabrina Shore, Alexandre Lebedev, Elena Hidalgo Ashrafi, Gerald Zon, Natasha Paul, Richard Hogrefe

Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.

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Metal Polymers, A Glue to Immobilise Proteins Onto Synthetic Surfaces
Abernethy N, Chung E, Fontanelle BT, Gao Y, Jennins D, Koudijs MM, Lim D, Yang L, Ling T, Vukovic P, Wong A, Maeji, NJ

The main objective of this work was to develop a surface chemistry which maintains protein function and orientation per unit surface area, regardless of the surface used.

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Identification of Novel BACE-1 Inhibitors through an Advanced Structure-Based Virtual Screening Protocol
Puneet Kacker, Angela De Simone, Matteo Masetti, Giovanni Bottegoni, Vincenza Andrisano, Andrea Cavalli

This study assesses the influence of different ligand chemotypes on the protonation state of the catalytic dyad.

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Showing Results 41 - 50 of 147
Scientific News
Repressing the Repressors May Drive Tissue-Specific Cancers
Stowers scientists establish Drosophila and mammalian models to study mutations found in pediatric brain tumors.
MU Researchers Discover Protein's Ability To Inhibit HIV Release
TIM-family proteins have the ability to block the release of HIV and other viruses.
Epigenetic Study Bolsters Alzheimer's Understanding
The current study found that chemical modifications to DNA within the ANK1 gene are strongly associated with measures of neuropathology in the brain.
Identification of Psoriatic Arthritis Mediators in Synovial Fluid
This study represents the most comprehensive proteomic analysis of psoriatic arthritis synovial fluid, to date.
GTEx Project to Expand Functional Studies of Genomic Variation
Larger set of human tissues to be analyzed to contribute to a database and tissue bank that researchers can use to study how genomic variants influence gene activity.
Subcellular Imaging Visualizes Structures of Brain Receptors
The advance opens a new window to study protein interactions in cell membranes in exquisite detail.
UTSW Cancer Researchers Identify Irreversible Inhibitor for KRAS Gene Mutation
Irreversible inhibitor for KRAS gene mutation involved in lung, colon, and pancreatic cancers.
UK Government Must Do More to Reduce Risk of vCJD Infection
Report recommends that UK Government commission a full assessment of the risks currently faced by the UK blood supply.
Capturing Cancer: A Powerful New Technique for Early Diagnosis
Researchers describe an innovative technique for early disease detection, which they call immunosignaturing.
Significant Step Towards Blood Test for Alzheimer's
Scientists have identified a set of 10 proteins in the blood which can predict the onset of Alzheimer’s, marking a significant step towards developing a blood test for the disease.
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