|Targeting Inflammatory Cytokines Using Adenoviruses: gene delivery of biological therapies in ovarian cancer|
Michael A. Salako, Hagen Kulbe, Iain A. McNeish, Frances R. Balkwill
Constitutive TNF-alpha expression is characteristic of the malignant ovarian surface epithelium. Adenoviral mutants hold great promise as gene therapy vectors but their efficacy is hindered by an inflammatory cascade orchestrated by TNF-alpha. We found that delivering TNF-alpha shRNA to ovarian cancer cells using oncolytic adenoviruses could reduce the inflammatory cascade generated by adenoviruses and also had direct anti-tumour activity on the cancer cells.
|Evolution of the Contact Phase of Vertebrate Blood Coagulation|
M. B. Ponczek, D. Gailani and R. F. Doolittle
Three proteases involved in the contact phase of vertebrate blood coagulation were searched in genomes of amphibians, birds and mammals. Several data sources were used during this bioinformatics analysis. Our survey confirmed that the vertebrate contact phase has occurred by way of a series of gene duplications during the course of several hundred million years of evolution, beginning with the appearance of fXII and a PK-fXI predecessor in amphibians.
|Bayesian Parameter Estimation for Systems Biological Models of Dynamic Cellular Networks|
Jarad Niemi, Lingchong You and Mike West
Dynamic cellular networks determine production of proteins in the cells in our body. The Rb-E2F network is a key example: it controls cell cycle, proliferation and degradation - a key network in almost all cancers. We develop statistical models of such single-cell processes - fine-time scale dynamic network models. Model estimation requires informative priors and custom Metropolis proposals.
|Hyperbaric Bioreactors use with Yarrowia Lipolytica Cultures: Cellular Adaptation to Hyperbaric Conditions|
Marlene Lopes, Nelma Gomes, Manuel Mota, Isabel Belo
In this work, a pressurized bioreactor was used for Y. lipolytica batch cultivation under increased air pressure from 1 bar to 6 bar. Cell growth was strongly enhanced by the pressure rise. The increase of oxygen availability caused the induction of the antioxidant enzyme SOD. The pre-growth of Y. lipolytica under increased pressure conditions did not affect the lipase production ability of the cells.
|Treatment of Highland Frogs from the Two Legged Stage with Homeopathically Prepared Thyroxin (10-11 – 10-21)|
Gerhard Lingg1, P. Christian Endler1,*, Michael Frass1, and Harald Lothaller2
For the experiment presented here, we used the same setup as for the initial multilaboratory study[1,2,3,4], but a special preparation process of the homeopathic drug involving dilution steps of 1: 50,000 and leading to thyroxin 10–11 – 10–21 was used.
|A high-throughput protein array-based approach |
Rachel Van Dyk, Claudia Kirisits, Paul Potter, Fook Tim Chew, Reinhard Hiller
Here we present a novel discovery-oriented high-throughput approach to the screening of allergen reactivates in crude biological extracts is presented using seafood allergens as an example. The CPGR workflow permits the effective screening of hundreds of putative allergens in parallel using minute amounts of patient serum and constitutes a cost-efficient allergen-specific antibody screening method for a routine diagnostic setting.
|A Novel Fluorimetric Assay for the Detection of TACE (a-Secretase)|
Vera Rakhmanova, Xudong Zhu, Fengying Li, Xiaofen Zhong, Anita Hong and Xiaohe Tong
To facilitate high throughput screening of TACE inhibitors, AnaSpec synthesized a novel peptide substrate for TACE using QXL™ 520/5-FAM FRET pair. Using this FRET substrate, AnaSpec developed a new kit – the SensoLyte™ 520 TACE Activity Assay. This kit can be used to detect the activity of the enzyme and for screening of TACE inhibitors. It is highly sensitive and can detect subnanogram amounts of enzyme.
|Specific Ligand-Based Depletion of Human Albumin and IgG in the Presence of Denaturants|
Todd Sanderson, Hongshan Li, Brian Miller, Laura Alionte, and Lisa Bradbury
Poster data highlights the advantages of a ligand-based depletion method that functions in the presence of urea and is highly specific, disposable, cost effective, and flexible.
|A New Disposable, Specific Ligand-Based Method for Depletion of Albumin and IgG From Serum and Plasma|
Laura Alionte, Allison Miller, Cal Messing, Tao Hu, and Lisa Bradbury
Poster shows the advantages of a ligand-based depletion method that is highly specific, disposable, cost effective, and flexible. Pall’s Enchant Multi-Protein Affinity Separation Kit is a strong addition to the product choices for scientists engaged in biomarker discovery and proteomics research.