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Tuesday, July 29, 2014
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Some thiazole derivatives as potent antiproliferative agents with CDK2/Cycin E/A inhibitory activities
Mahesh Chhabria1, Shailesh Patel*1, Maulik Suthar2

We report synthesis and biological evaluation of novel thiazole derivatives. High throughput screening of various compounds for their anti-proliferative activity led to the identification of thiazolo-thiones as potential candidate for Lung cancer (NCl-H23, NClH510A, NCI-H522) and breast cancer (MDA-MB-453/231/468, MCF-7). The most potent compounds were also screened for their inhibitory activity against CDK2/ Cyclin E/A which are considered as promising targets for lung and breast cancer.

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Predicting hepatotoxicity: Reactive metabolite trapping using glutathione and freshly isolated hepatocytes
Birks, V., Webber, G., Geoffroy, S., Cole, R., and Wood, S.

This poster presents our results to date using clozapine (a compound known to be associated with GSH-adduct formation) as substrate and using stable isotope GSH (GSH13C2,15N) to enhance specificity. In addition, all analyses have been conducted using an Waters Acquity UPLC-MS/MS. Results we have obtained in hepatocytes are compared against findings using human liver microsomes (HLM).

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A simple, fast and quantitative single-step dead-cell indicator for flow cytometry
Jixiang Liu, Jolene Bradford, Chris Langsdorf

We have evaluated a series of new compounds for dead cell stain and identified a new product, SYTOX® AADvancedTM dead cell stain, which demonstrates improved properties over 7-AAD. These properties make the SYTOX® AADvancedTM dead cell stain a simple, fast and quantitative single-step no-wash dead-cell indicator as well as ideal for use in multicolor application requiring DNA content.

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Protein array-based screening of autoantibody signatures
Zingaretti C., Arigò M., Colombatto P., Brunetto M., Muratori L., Bonino F., Bianchi F., Pagani M., De Francesco R., Abrignani S., Bombaci M.

The evidence for an association between autoimmune diseases and chronic HCV infection has been clearly established. To this aim, a protein array was employed to analyze serum samples of HCV patients w/wo autoimmune complications, of patients with autoimmune hepatitis but not infected with HCV and of healthy donors as controls. A panel of autoantigens able to discriminate among the three groups of patients was identified for potential use as biomarkers.

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PROTEOME WIDE PLASMA PROFILING USING ANTIBODY SUSPENSION BEAD ARRAYS
Maja Neiman, Ulrika Igel, Burcu Ayoglu, Kimi Drobin, Mathias Uhlén, Peter Nilsson and Jochen M. Schwenk

A newly developed antibody suspension bead array assay allows for a systematic and high-throughput plasma profiling. This microtiter based assay uses antibody-coupled beads for a multiplexed analysis of minute amounts of directly labelled samples. The key requirement of a?nity reagents towards all human proteins is met by the Human Protein Atlas project.

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Five noncovalent peptidic ligands show different affinity rankings in solution and gas phase
Andrey Dyachenko , Michael Goldflam , Marta Vilaseca , Ernest Giralt

Stability of noncovalent complexes of VEGF protein with 5 peptidic ligands is studied. Experiments were conducted in solution (NMR CSP, ITC) and in gas phase (CID TOF MS). Each ligand differs from others in chirality of one amino acid. It was shown, that trend of stability of the studied noncovalent complexes is reversed in the gas phase relatively to the solution. An explanation of this behavior is presented.

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Software for Genomic/Epigenomic Research
A.S.Tanas, V.V.Shkarupo, E.B.Kuznetsova, D.V.Zaletayev, V.V. Strelnikov

We present two computer programs: 1) AIMS in silico - a suggestion tool for Amplification of Intermethylated Sites experimental design and results analysis; and 2) PeakPick – a tool to view & analyse capillary electrophoregrams Our computer software is intended to standardize AIMS applications and to turn it into one of the principal approaches towards cancer methylomes studies as well as towards diagnostics in oncology, including early screening.

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Insulin Decreases Transcription of Three Proteins Associated with Lung Surfactant
Rucka, Z [a], Vanhara, P [b], Tesarova, L [a], Potesilova, M [a], Stejskal, S [a], Dolezel, J [c], Koutna, I [a]

Our project is partially focused on insulin affecting four proteins associated with lung surfactant. Small and hydrophobic SP-B and S-PC help to spread and stabilize the surfactant layer while large and hydrophilic SP-A and SP-D participate in immune responses. A549 and H441 cell lines were used. It was observed using RealTime PCR that higher doses of insulin lead to decreased transcription of SP-B, SP-C, SP-D, and both isoforms of SP-A.

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Synthesis and trafficking of the tonoplast potassium channel AtTPK1
Marie Maitrejean (1,2), Michael Wudick (2), Camilla Voelker (2), Katrin Czempinski (2), Emanuela Pedrazzini (1), Alesandro Vitale (1)

Sorting signals of tonoplast proteins and the pathway they follow through the endomembrane system are still poorly characterized. Using Brefeldin A (an inhibitor of the Golgi-mediated traffic) we showed that a Golgi-dependent pathway for tonoplast delivery may exist. Then we generated various chimeric TPK1-TPK4 fusions, exchanging various domains. We observed that the cytosolic, C-terminal domain of TPK1 is sufficient to re-direct the plasma membrane TPK4 to the tonoplast.

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Showing Results 61 - 70 of 143
Scientific News
UTSW Cancer Researchers Identify Irreversible Inhibitor for KRAS Gene Mutation
Irreversible inhibitor for KRAS gene mutation involved in lung, colon, and pancreatic cancers.
UK Government Must Do More to Reduce Risk of vCJD Infection
Report recommends that UK Government commission a full assessment of the risks currently faced by the UK blood supply.
Capturing Cancer: A Powerful New Technique for Early Diagnosis
Researchers describe an innovative technique for early disease detection, which they call immunosignaturing.
Significant Step Towards Blood Test for Alzheimer's
Scientists have identified a set of 10 proteins in the blood which can predict the onset of Alzheimer’s, marking a significant step towards developing a blood test for the disease.
Researchers Identify Urgent Need for Alzheimer's Disease Drug Development
Analysis of clinical trials published in the journal Alzheimer's Research & Therapy shows that the pipeline for potential treatments of AD is small and rate of success is limited.
New Tool to Study Critical Protein Interaction in Cancer Research
A*STAR scientists used fluorescent molecular rotors to study protein-protein interactions involving p53 and MDM2 in cells.
Researchers Discover ‘Trojan Horse’ Method
Scientists found a way to deliver proteins into live human cells without harm.
Missing Protein Explains Link Between Obesity and Diabetes
A*STAR scientists pioneered a molecular connection between the two health conditions.
Potential Alzheimer’s Drug Prevents Abnormal Blood Clots in the Brain
Researchers have identified a compound that might halt the progression of Alzheimer’s by interfering with the role of plaque forming amyloid-ß.
CRI Finds Key to Identifying, Enriching Mesenchymal Stem Cells
Biomarker enables researchers to accurately characterize the properties and function of MSCs in the body.
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