Posters

The presence of albumin in the urine of healthy subjects presents a stumbling block in diagnostics putting a question mark on the validity of albumin test for the detection of diabetic nephropathy. Therefore there arises a dire need to hunt for the proteins in the pertinent bio- fluids which can serve as biomarkers.

Proteome Systems has identified TB proteins in sputa and blood of people diagnosed with TB. Several of these proteins have been prioritised for ongoing validation. This involves generation of sensitive antibodies and optimisation of assays for detection of TB antigens in clinical samples. This presentation will discuss our progress in this program and our strategy for incorporation of these markers into a diagnostic test format.

Breast cancer has diverse metastatic behavior. Subpopulations of cells with enhanced metastatic abilities either to bone (1833) or to lung (4175) have been isolated by the group of J. Massague by in vivo selection of MDA-MB-231 cells. We performed the 2-DE analysis of the cell lines. We have found and identified 11 differentially expressed protein spots.

The correpressor Alien is shown to interact with several proteins involved in regulation of transcription, DNA repair and cell cycle. Furthermore we found E2F1 transcription to be downregulated by Alien overexpression.

Increased nitric oxide (NO) production has been implicated in the pathogenesis of the hyperdynamic circulation found in patients with advanced liver disease. There were no differences between patients with liver disease and controls with regard whole body NO production. Our results, in a well characterised set of patients, argue against greater basal NOS-dependent whole body NO production in patients with decompensated liver disease.

LC-MS based proteomic experiments are used to compare complex biological samples across multiple conditions. Fast, powerful computational tools are needed to explore and detect differences in the areas of Expression Proteomics and Biomarker Discovery. In general, specialized steps are necessary to solve these difficult problems (binning, alignment & normalization, peak picking, relative quantitation, etc.). MS-Xelerator is a collection of software tools dedicated to all of the above tasks.

In the context of determination of the proteome of Arabidopsis Thaliana chloroplast we will present number of identified peptides and its relationship to the peak capacity of the chromatographic systems based on packed or monolithic columns.

Caveolin1 (Cav-1) function either as a tumor supressor or as a promoter of metastasis. Overexpresion of cav-1 was correlated with: tumoral grading, proliferration markers (Ki67, p53), serum tumor markers (CEA, CA19.9) and angiogenic markers (VEGF, bFGF).

Benzothiazole-2-sulfonyl (Bts) has been reported as an amine-protecting group of amino acid, and the stability of sulfonamide and the mild deprotection conditions are attractive properties for a protecting group. Due to the strong electron-withdrawing effect of the sulfonyl group, the acyl group of acylsulfonamideis easily attacked by nucleophiles after alkylation which was applied in the safety-catch strategy or the synthesis of a peptide thioester for native chemical ligation of the peptide.