Posters

We present a sandwich-injection method for controlling discrete sample injection in µ-CE. This method involves four accessory arm channels in which symmetrical potentials are loaded to form a unique parallel electric field distribution to confine the spreading of sample plugs and prevent sample leakage. The virtues of the novel injection technique were demonstrated with numerical models and experiments.

UniProt (Universal Protein Resource) provides a central resource on protein sequences and functional annotation. The UniProt Consortium is comprised of the European Bioinformatics Institute (EBI), the Swiss Institute of Bioinformatics (SIB) and the Protein Information Resource (PIR). The UniProt Knowledgebase (UniProtKB) contains the manually annotated UniProtKB/Swiss-Prot section and the automatically annotated UniProtKB/TrEMBL section.

UniProt (Universal Protein Resource) provides a central resource on protein sequences and functional annotation. The UniProt Consortium is comprised of the European Bioinformatics Institute (EBI), the Swiss Institute of Bioinformatics (SIB) and the Protein Information Resource (PIR). The UniProt Knowledgebase (UniProtKB) contains the manually annotated UniProtKB/Swiss-Prot section and the automatically annotated UniProtKB/TrEMBL section.

UniProt (Universal Protein Resource) provides a central resource on protein sequences and functional annotation. The UniProt Consortium is comprised of the European Bioinformatics Institute (EBI), the Swiss Institute of Bioinformatics (SIB) and the Protein Information Resource (PIR). The UniProt Knowledgebase (UniProtKB) contains the manually annotated UniProtKB/Swiss-Prot section and the automatically annotated UniProtKB/TrEMBL section.

In this study we compare methods for large-scale microarray analysis of protein and RNA level changes in HL-60 cells, responding to differentiation stimuli. Using microarrays we have found, that level of several proteins was either up- or down-regulated after cell differentiation. In some cases there was significant correlation with appropriate genes.

In order to facilitate high throughput screening of AD drug candidates, we have developed a new SensoLyte™ 520 b-secretase assay kit using a fluorescence resonance energy transfer (FRET) peptide, HiLyte Fluor™ 488-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(QXL™ 520)-OH. The longer excitation and emission wavelengths of HiLyte Fluor™488 minimize the interference from autofluorescence and absorbance of test compounds.

We demonstrate highly-selective and sensitive labeling methods for the detection of specific glycoprotein subclasses, including cell surface N- and O-linked glycoproteins and intracellular O-GlcNAc modified proteins, utilizing the copper-catalyzed cycloaddition reaction between azides and alkynes, or click chemistry.

Cell chips are developed to continuously monitor mammalian cell population dynamics in a non-invasive manner. In the presented work we describe the design, fabrication and characterization of a lab-on-a-chip for quantitative cell analysis.

Microfabricated biochips are developed to continuously monitor cell population dynamics in a non-invasive manner. In the presented work we describe the novel combination of contact-less dielectric microsensors and microfluidics to promote biofilm formation for quantitative cell analysis.