The MaxDiscovery™ Apolipoprotein B (ApoB) ELISA Kit is an enzyme immunoassay that analyzes the quantity of ApoB in cells, tissues, serum, plasma or cultured cells. Apoliporotein B is the primary apoliporotein of low-density lipoproteins (LDL), which is responsible for carrying cholesterol to tissues. Apoliporotein B acts as a ligand for LDL receptors in various cells throughout the body. The protein has two main isoforms, ApoB-48 and ApoB-100. In humans ApoB-48 is synthesized in the small intestine while ApoB-100 is made in the liver. There is significant evidence that shows that high levels of Apolipoprotein B can lead to plaques that cause atherosclerotic coronary disease. In fact, Apolipoprotein B levels are thought to be an even better indicator of heart disease risk than total cholesterol or LDL. Bioo Scientific’s ApoB ELISA kit is an immunoassay that can be used to measure ApoB levels in cells, serum and tissue with minimal sample preparation. The standard curve spans the range of 0-200 ng/mL. Like most ELISA assays, the MaxDiscovery™ ApoB ELISA Test relies on a Horseradish Perioxidase (HRP) conjugated antibody and the TMB (3,3´,5,5´-tetramethylbenzidine) substrate. TMB is a chromogen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) that has major absorbances at 370 nm and 652 nm. The color then changes to yellow with the addition of acid with maximum absorbance at 450 nm. The relative amount of ApoB protein in the cells will be directly proportional to the amount of signal that is obtained at 450 nm.
Retractable Protein Nanoneedles The ability to control the transfer of molecules through cellular membranes is an important function in synthetic biology; a new study from researchers at Harvard’s Wyss Institute for Biologically Inspired Engineering and Harvard Medical School (HMS) introduces a novel mechanical method for controlling release of molecules inside cells.A Crystal Clear View of Biomolecules Fundamental discovery triggers paradigm shift in crystallography.Charting Kidney Cancer Metabolism Changes in cell metabolism are increasingly recognized as an important way tumors develop and progress, yet these changes are hard to measure and interpret. A new tool designed by MSK scientists allows users to identify metabolic changes in kidney cancer tumors that may one day be targets for therapy."Dark Side" of the Transcriptome New approach to quantifying gene "read-outs" reveals important variations in protein synthesis and has implications for understanding neurodegenerative diseases.Advancing Synthetic Biology Living systems rely on a dizzying variety of chemical reactions essential to development and survival. Most of these involve a specialized class of protein molecules — the enzymes.Structure of Brain Plaques in Huntington's Researchers at the University of Pittsburgh School of Medicine have shown that the core of the protein clumps found in the brains of people with Huntington's disease have a distinctive structure, a finding that could shed light on the molecular mechanisms underlying the neurodegenerative disorder. Visualizing a Cancer Drug Target at Atomic Resolution Using cryo-electron microscopy, researchers were able to view, in atomic detail, the binding of a potential small molecule drug to a key protein in cancer cells.Pumpjack" Mechanism for Splitting and Copying DNA High-resolution structural details of cells' DNA-replicating proteins offer new insight into how these molecular machines functionThe Power of Three Overlooked portion of cell “death receptor” critical in some cancers, autoimmune diseases.Biomarker for Recurring HPV-Linked Oropharyngeal Cancers A look-back analysis of HPV infection antibodies in patients treated for oropharyngeal (mouth and throat) cancers linked to HPV infection suggests at least one of the antibodies could be useful in identifying those at risk for a recurrence of the cancer, say scientists at the Johns Hopkins University.