Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>Products>This Product

MaxDiscovery™ GAPDH ELISA Kit

Product Description
The MaxDiscovery™ Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) ELISA Kit is an enzyme immunoassay that analyzes the quantity of GAPDH in cells, tissues, serum or urine. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH initiates the second stage of glycolysis, catalyzing the reaction that converts glyceraldehyde 3-phosphate (GAP) into 1,3 bisphosphoglycerate (1,3 BPG). GAPDH oxidizes and phosphorylates GAP to produce 1,3 BPG, which is then used as an intermediate in the synthesis of ATP. While the glycolytic function of GAPDH is widely known, recent evidence suggests that GAPDH is a highly versatile molecule that plays several diverse roles in living systems. Mammalian GAPDH is involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. There have also been many findings that GAPDH plays a role in different pathologies including prostate cancer progression, programmed neuronal cell death and age-related neuronal diseases, i.e. Alzheimer’s and Huntington’s disease. The GAPDH gene is constitutively expressed at high levels in almost all tissues. However, the molecular mechanism that sustains high-level expression of this housekeeping enzyme is still unclear. GAPDH is almost always a tetramer and is localized to the cytoplasm in healthy cells. Translocation of GAPDH into the nucleus is seen during its role in the early stages of apoptosis and oxidative stress. Because of its high-level and constitutive expression, GAPDH is widely used as a loading control for Northern/Western blots and for protein normalization. Like most ELISA assays, the MaxDiscovery™ GAPDH ELISA Test relies on a Horseradish Perioxidase (HRP) conjugated antibody and the TMB (3,3´,5,5´-tetramethylbenzidine) substrate. TMB is a chromogen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) that has major absorbances at 370 nm and 652 nm. The color then changes to yellow with the addition of acid with maximum absorbance at 450 nm. The relative amount of GAPDH protein in the cells will be directly proportional to the amount of signal that is obtained at 450 nm. Selected Citations: Jones, J. A. et al. (July, 2010) Alterations in membrane type-1 matrix metalloproteinase abundance after the induction of thoracic aortic aneurysm in a murine model. Am J Physiol Heart Circ Physiol, 299: H114 - H124. doi: 10.1152/ajpheart.00028.2010
Product MaxDiscovery™ GAPDH ELISA Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 1 x 96 wells
Quantity 510
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122

Scientific News
Resurrected Proteins Double Their Natural Activity
Researchers demonstrate method for reviving denatured proteins.
Scientists Decode Structure at Root of Muscular Disease
Researchers at Rice University and Baylor College of Medicine have unlocked the structural details of a protein seen as key to treating a neuromuscular disease.
Sniffing Out Cancer
Scientists have been exploring new ways to “smell” signs of cancer by analyzing what’s in patients’ breath.
A New Single-Molecule Tool to Observe Enzymes at Work
A team of scientists at the University of Washington and the biotechnology company Illumina have created an innovative tool to directly detect the delicate, single-molecule interactions between DNA and enzymatic proteins.
Milestone Single-Biomolecule Imaging Technique May Advance Drug Design
The first nanometer resolved image of individual tobacco mosaic virions shows the potential of low-energy electron holography for imaging biomolecules at a single particle level; a milestone in structural biology and a potential new tool for drug design.
Researchers Discover A New Mechanism of Proteins to Block HIV
Certain IFITM proteins block and inhibit cell-to-cell transmission of HIV.
A Natural Light Switch
MIT scientists identify and map the protein behind a light-sensing mechanism.
Biologists Find Unexpected Role for Amyloid-Forming Protein
Yeast protein could offer clues to how Alzheimer’s plaques form in the brain.
Study Adds to Evidence That Viruses Are Alive
A new analysis supports the hypothesis that viruses are living entities that share a long evolutionary history with cells, researchers report.
How Flu Viruses Gain The Ability To Spread
New study reveals the soft palate is a key site for evolution of airborne transmissibility.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos