Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>Products>This Product

MaxDiscovery™ ß-Galactosidase ELISA Kit

Product Description
The MaxDiscovery™ ß-Galactosidase ELISA Kit is an enzyme immunoassay that analyzes the quantity of ß-galactosidase in cells, tissues or serum. ß-galactosidase is a hydrolase enzyme that catalyzes the hydrolysis of ß-galactosides into monosaccharides. The ß-galactosidase protein is a large (120 kDa, >1000 amino acids) protein that forms a tetramer. In E. coli the ß-galactosidase protein is encoded by the lacZ gene which is part of the lac operon. The enzyme's function in E. coli is to cleave the disaccharide lactose to form glucose and galactose so they can be used as carbon/energy sources. The lacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of gene promoters. ß-galactosidase is not typically expressed in mammalian cells, however; ß-galactosidase positive staining is a marker for mammalian cellular senescence. ß-galactosidase is a useful reporter gene both in cell culture and in vivo applications due to its ease of detection. Like most ELISA assays, the MaxDiscovery™ ß-Galactosidase ELISA test relies on a Horseradish peroxidase (HRP) conjugated primary antibody and the TMB (3,3´,5,5´-tetramethylbenzidine) substrate. TMB is a chromogen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) and has a major absorbance at 370 nm and 652 nm. The color then changes to yellow with the addition of acid with maximum absorbance at 450 nm. The relative amount of ß-galactosidase protein in the cells will be directly proportional to the amount of signal that is obtained and thus is an excellent technique for detecting protein expression within cell lines.
Product MaxDiscovery™ ß-Galactosidase ELISA Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 1 x 96 wells
Quantity 510
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122

Scientific News
Smartphone Laboratory Detects Cancer
Researchers develop low-cost, portable laboratory on a smartphonecapable of analysing multiple samples simultaneously.
RNA-Binding Proteins Role in ALS Revealed
Researchers describe how damage to RNA-binding protein contributes to ALS, isolating a possible therapeutic target.
Advances in Alzheimer’s Research
Researchers show how a diseased vertebrate brain can naturally react to Alzheimer’s pathology by forming more neurons.
Study Finds Key Regulator in Pulmonary Fibrosis
Researchers identify an enzyme that could open the way to therpies for chronic fatal lung disease.
NIH Study Determines Key Differences between Allergic and Non-Allergic Dust Mite Proteins
Researchers at NIH have uncovered factors that lead to the development of dust mite allergy and assist in the design of better allergy therapies.
Integrated Omics Analysis
Studying multi-omics promises to give a more holistic picture of the organism and its place in its ecosystem, however despite the complexities involved those within the field are optimistic.
Alzheimer’s-Linked Protein May Play Role in Schizophrenia
Researchers suggests a protein linked to cognitive decline in Alzheimer's also plays a role in genetic predisposition to schizophrenia.
Peptides vs. Superbugs
Scientists successfully develop a shuttle system made of liquid-crystalline nanomaterials that protect peptites.
Cocoa Compound Linked to Some Cardiovascular Biomarker Improvements
The study highlights the urgent need for large, long-term RCTs that improve understanding of how the short-term benefits of cocoa flavanol intake on cardiometabolic biomarkers may be translated into clinical outcomes.
Immune Approach Targets Humans Instead of Bacteria
Scientists show for the first time how bacterial superantigen toxins work, and how short peptides can block them and save lives.
Scroll Up
Scroll Down

Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,200+ scientific videos