The MaxDiscovery™ Human IL-17 ELISA Test Kit is designed for quantitative determination of the concentration of human IL-17 in serum, plasma, and cell culture supernatant. Human interleukin 17 (IL-17) is a disulfide-linked homodimer with each ~ 17.5 kDa polypeptide of 136 amino acids (predicted mass = 17.5 kDa) after cleavage of a signal sequence of 19 amino acids. It contains six cysteines plus one potential N-linked glycosylation site, which is variably glycosylated, at least with recombinant proteins. It shares 63% and 58% sequence identity to those of mouse and rat, respectively and 72% identical to the thirteenth ORF of Herpesvirus saimiri. Originally identified as mouse cytotoxic T lymphocyte-associated antigen-8 (CTLA-8), IL-17 is produced by activated memory T lymphocytes. IL-17 appears to mediate communication between the immune system and the hematopoietic system. There is at least some species specificity for in vitro action on bone-marrow stromal cells. The IL-17 receptor (IL-17 R) from the mouse has been characterized. It is a 120 kDa type I transmembrane glycoprotein. There is neither an Ig-like domain nor the WSXWS motif of hematopoietin receptors in the 291 amino acid extracellular segment of the receptor, and there is no identifiable tyrosine kinase domain in the 521 residue cytosolic region. There are, however, serine-rich regions similar to those in IL-2 Rß, IL-4 R and G-CSF R. Thus, the receptor appears to be related to class I cytokine receptors. Based on cell line studies, IL-17 R is expressed by mast cells, fibroblasts, fetal hepatocytes, pre-B cells, and intestinal epithelial cells. It has been reported that IL-17 mediated T cell communication with the hematopoietic system. T cell-derived IL-17 induces fibroblasts to produce IL-6, IL-8, ICAM-1 and G-CSF, apparently by an NF-?B-mediated mechanism. IL-6 in turn promotes development of granulocyte/macrophage colonies, and G-CSF directs development of neutrophils. IL-17 also enhances proliferation of partially activated T cells and upregulates nitric oxide (NO) production in osteoarthritic cartilage. Selected Citations: Akhtar N., Verma, K. K., Sharma A. (Oct 2010) Study of pro- and anti-inflammatory cytokine profile in the patients with parthenium dermatitis Contact Dermatitis 63 (4): 203 – 208. Akhtar, N. et al (Dec 2010)Dysregulation of TH type cytokines in the patients of Parthenium induced contact dermatitis. Clinica Chimica Acta, 411 (23-24): 2024 - 2028.
Retractable Protein Nanoneedles The ability to control the transfer of molecules through cellular membranes is an important function in synthetic biology; a new study from researchers at Harvard’s Wyss Institute for Biologically Inspired Engineering and Harvard Medical School (HMS) introduces a novel mechanical method for controlling release of molecules inside cells.A Crystal Clear View of Biomolecules Fundamental discovery triggers paradigm shift in crystallography.Charting Kidney Cancer Metabolism Changes in cell metabolism are increasingly recognized as an important way tumors develop and progress, yet these changes are hard to measure and interpret. A new tool designed by MSK scientists allows users to identify metabolic changes in kidney cancer tumors that may one day be targets for therapy."Dark Side" of the Transcriptome New approach to quantifying gene "read-outs" reveals important variations in protein synthesis and has implications for understanding neurodegenerative diseases.Advancing Synthetic Biology Living systems rely on a dizzying variety of chemical reactions essential to development and survival. Most of these involve a specialized class of protein molecules — the enzymes.Structure of Brain Plaques in Huntington's Researchers at the University of Pittsburgh School of Medicine have shown that the core of the protein clumps found in the brains of people with Huntington's disease have a distinctive structure, a finding that could shed light on the molecular mechanisms underlying the neurodegenerative disorder. Visualizing a Cancer Drug Target at Atomic Resolution Using cryo-electron microscopy, researchers were able to view, in atomic detail, the binding of a potential small molecule drug to a key protein in cancer cells.Pumpjack" Mechanism for Splitting and Copying DNA High-resolution structural details of cells' DNA-replicating proteins offer new insight into how these molecular machines functionThe Power of Three Overlooked portion of cell “death receptor” critical in some cancers, autoimmune diseases.Biomarker for Recurring HPV-Linked Oropharyngeal Cancers A look-back analysis of HPV infection antibodies in patients treated for oropharyngeal (mouth and throat) cancers linked to HPV infection suggests at least one of the antibodies could be useful in identifying those at risk for a recurrence of the cancer, say scientists at the Johns Hopkins University.