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ULTImate Y2H™

Product Description
ULTImate Y2H™ is an optimized version of the yeast two-hybrid (Y2H) screening technology that tackles most of the false positives and false negatives (Rain JC et al., Nature 2001, 409: 211-215). Our offer includes:

- Bait design, benefit from our experience and optimize your chances of success
- Bait cloning, with the newly created bait fully sequence-checked
- Small scale screen
* to test and overcome bait toxicity
* to test autoactivation and select the most appropriate screening conditions

- Full scale screen, to test 83 million interactions on average by cell-to-cell mating on the most complex domain libraries available
- 134 fully analysed positive clones delivered on average per screen
- Sophisticated analysis of the data
* sequence and identification of all positive clones
* confidence score attributed to each interaction
* delineation of the minimal interacting domain on each prey and comparison with functional and structural domains present on the protein
- A second screen at an higher expression level is performed when less than 20 clones are identified in the first screen.

For more details on ULTImate Y2H, please click here
Product ULTImate Y2H™
Company Hybrigenics Services SAS
Price Request a quote
More Information View company product page
Catalog Number Unspecified
Quantity Unspecified
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Hybrigenics Services SAS
3-5 Impasse Reille 75014 Paris France

Tel: +33 (0)1 58 10 38 29
Fax: +33 (0)1 58 10 38 48
Email: services@hybrigenics.com



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