ULTImate Y2H™ is an optimized version of the yeast two-hybrid (Y2H) screening technology that tackles most of the false positives and false negatives (Rain JC et al., Nature 2001, 409: 211-215). Our offer includes:
- Bait design, benefit from our experience and optimize your chances of success - Bait cloning, with the newly created bait fully sequence-checked - Small scale screen * to test and overcome bait toxicity * to test autoactivation and select the most appropriate screening conditions
- Full scale screen, to test 83 million interactions on average by cell-to-cell mating on the most complex domain libraries available - 134 fully analysed positive clones delivered on average per screen - Sophisticated analysis of the data * sequence and identification of all positive clones * confidence score attributed to each interaction * delineation of the minimal interacting domain on each prey and comparison with functional and structural domains present on the protein - A second screen at an higher expression level is performed when less than 20 clones are identified in the first screen.
For more details on ULTImate Y2H, please click here