Our Loss of Affinity Mutants (interaction-deficient) assay is based on ULTImate Y2H™. It consists in screening a bait against a library of mutant preys generaded from a known partner.
The mutagenic conditions used to generate the library are adapted to your protein in order to obtain a single mutation per clone on average.The sequences of 200 clones showing no or weak interaction are aligned and interaction “hot spots” characterized. You receive up to 15 different mutants that fail to interact.
Use our LAM screen service to map interaction hotspots and obtain dominant negatives to speed up your functional studies!