Corporate Banner
Satellite Banner
Proteomics
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


1by1 with HTRF®

Product Description
1-by-1 with HTRF interaction assay is based on Homogeneous Time-Resolved Fluorescence, a highly sensitive and robust Time-Resolved FRET technique. This service allows you to test for direct binding in vitro.

Our offer includes :
- Scientific assistance from our protein-interaction experts
- Cloning
- Protein production and purification: both candidate proteins are produced in bacteria as fusions to different tags
- Protein labelling: each protein is labelled either directly or indirectly with anti-tag antibodies coupled to fluorescence donor and acceptor
- Competition test: to control that proteins are well recognized by the antibodies and to evaluate the optimal protein concentrations.
- HTRF interaction test
- Detailed report delivery with protocols, gel pictures, grpahics and comments

Our multi-tag approach and the use of the two donor-acceptor orientations for each protein pair allow for systematically investigating a large panel of combinations, thus increasing the chance of proper protein folding and fluorescence transfer.

Because the assay is homogeneous and requires only low nanomolar concentrations of proteins, multiple interactions can be evaluated simultaneously in a 384-well format.

This makes 1-by-1 with HTRF the perfect output to confirm ULTImate Y2H™ interactions in another system for publication! Our HTRF and Y2H plasmids are fully compatible for direct and efficient subcloning.

For more details, please go to: http://www.hybrigenics-services.com/contents/our-services/validate/1-by-1-with-htrf

HTRF® is a registered trademark of Cisbio International.

Product 1by1 with HTRF®
Company Hybrigenics Services SAS
Price Request a quote
More Information View company product page
Catalog Number Unspecified
Quantity Unspecified
Company Logo

Hybrigenics Services SAS
3-5 Impasse Reille 75014 Paris France

Tel: +33 (0)1 58 10 38 29
Fax: +33 (0)1 58 10 38 48
Email: services@hybrigenics.com



Scientific News
HIV Particles Used to Trap Intact Mammalian Protein Complexes
Belgian scientists from VIB and UGent developed Virotrap, a viral particle sorting approach for purifying protein complexes under native conditions.
Potential “Good Fat” Biomarker
New method to measure the activity of energy consuming brown fat cells could ease the testing weight loss drugs.
Computational Model Finds New Protein-Protein Interactions
Researchers at University of Pittsburgh have discovered 500 new protein-protein interactions (PPIs) associated with genes linked to schizophrenia.
New Insights into Gene Regulation
Researchers have solved the three-dimensional structure of a gene repression complex that is known to play a role in cancer.
Controlling RNA in Living Cells
Modular, programmable proteins can be used to track or manipulate gene expression.
Soy Shows Promise as Natural Anti-Microbial Agent
Soy isoflavones and peptides may inhibit the growth of microbial pathogens that cause food-borne illnesses, according to a new study from University of Guelph researchers.
Potential Target for Revolutionary Antibiotics
An international team of including the Lomonosov Moscow State University researchers discovered which enzyme enables Escherichia coli bacterium (E. coli) to breathe.
DNA Barcodes Gone Wild
A team of researchers at University of Toronto’s Donnelly Centre and Sinai Health System’s Lunenfeld-Tanenbaum Research Institute (LTRI) has developed a new technology that can stitch together DNA barcodes inside a cell to simultaneously search amongst millions of protein pairs for protein interactions.
Biomarkers for Profiling Prostate Cancer Patients
Exiqon A/S has announced the publication of validation of prognostic microRNA biomarkers for the aggressiveness of prostate cancer in independent cohorts.
Grant to Fund Million Peaks Project
The European Research Council (ERC) has awarded a prestigious Advanced Grant to Prof. Peter Schoenmakers, Prof. Albert Polman and Prof. Huib Bakker, all three of whom work at the University of Amsterdam (UvA).
Scroll Up
Scroll Down

SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!