MBmate Y2H* screen is a version of the yeast two hybrid (Y2H) system dedicated to the screening of integral membrane proteins. It is based on the Split-Ubiquitin system (Stagljar I et al., PNAS 1998, 95: 5187-5192) and is optimized with our patented cell-to-cell mating technology.
Our offer includes: - Scientific assistance from our yeast two hybrid experts - Determination of the orientation of the bait fusion (N or C terminal), choice of the library for the screening - Cloning of your gene into the bait vector. - A functional test to check for the correct localization of your bait in the membrane - Screening of your bait against a cDNA library of your choice with our cell-to-cell mating technology - Sequencing of up to 380 positive clones. We deliver the sequences of each clone together with the Blast analysis and information about the interaction and functional domains present in the prey proteins.
Natural Protein Points to New Inflammation Treatment Findings may offer insight to effective treatments for inflammatory diseases, such as rheumatoid arthritis, psoriasis, and multiple sclerosis.Natural Protein Points to New Inflammation Treatment Findings may offer insight to effective treatments for inflammatory diseases, such as rheumatoid arthritis, psoriasis, and multiple sclerosis.Enzyme Links Age-Related Inflammation, Cancer Researchers have shown that an enzyme key to regulating gene expression -- and also an oncogene when mutated -- is critical for the expression of numerous inflammatory compounds that have been implicated in age-related increases in cancer and tissue degeneration. New Biomarker to Assess Stem Cells Developed A research team led by scientists from UCL have found a way to assess the viability of 'manufactured' stem cells known as induced pluripotent stem cells (iPSCs). The team's discovery offers a new way to fast-track screening methods used in stem cell research.Therapy Halts Progression of Lou Gehrig’s Disease Researchers at Oregon State University announced today that they have essentially stopped the progression of amyotrophic lateral sclerosis (ALS), or Lou Gehrig’s disease, for nearly two years in one type of mouse model used to study the disease – allowing the mice to approach their normal lifespan.Crouching Protein, Hidden Enzyme A new study led by scientists at The Scripps Research Institute (TSRI) and the University of California (UC), Berkeley shows how a crucial molecular enzyme starts in a tucked-in somersault position and flips out when it encounters the right target.HIV Protein Manipulates Hundreds of Human Genes Findings search for new or improved treatments for patients with AIDS.First 3-D Vision of Cancer Target This basic research set the grounds for structure-based drug design approaches that could be beneficial for cancer treatments' - Dr. Cyril Dominguez, University of Leicester.New Light Shed on Genetic Regulation A team of scientists has uncovered greater intricacy in protein signaling than was previously understood, shedding new light on the nature of genetic production.Hacking the Programs of Cancer Stem Cells All tumor cells are the offspring of a single, aberrant cell, but they are not all alike.