Our libraries are the most complex ones available on the market and our ULTImate cell-to-cell mating is the only screening technology that can screen them exhaustively. Should you not find your favourite library among our 60+ libraries, we would be happy to construct it for you.
Our ULTImate domain libraries are validated by extremely stringent quality controls: - Verifying a complexity of at least 10 million independent, primary fragments in yeast for Random-Primed cDNA libraries. The complexity of the genomic libraries (constructed for organisms that contain no or few introns) depends on the size of the genome (e.g. 2 million genomic fragments for Helicobacter pylori library) - Random sequencing of 192 clones to check for i) absence of contamination, ii) that ribosomal RNA, mitochondrial DNA and empty vectors represent less than 10% of the total sequences - Verifying the viability and mating properties of the library-containing yeast cells, and defining the conditions to obtain the highest mating efficiency (Patent US No 6,187,535) - Reproducing selected Y2H screen interactions available from the literature.