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SpikeTidesTM peptides – light – heavy – quantified. The most economic source for quantified heavy peptides!

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Product Description

JPT’s SpikeTidesTM are customized, small scale, proteotypic peptides. They are produced light or isotopically labeled and /or absolutely quantified. Libraries of thousands of peptides at economic pricing become available for the first time due to JPT’s proprietary synthesis and quantification technology.

Applications for SpikeTidesTMDSC_8236_klein.gif
- Identification & optimization of proteotypic peptides
- Development of SRM/MRM assays
- Absolute and relative quantification of protein expression levels
- Biomarker discovery and validation
- Clinical monitoring of therapeutic invention and disease status

Benefits of SpikeTidesTM
- Most economic source for proteotypic peptides
- Compatible with current proteomic workflows
- Unmatched turnaround times (10 000 peptides within 2 weeks!)
- Delivery in ready-to-use microtiter plates
- JPT’s quantification method is as robust and much more cost effective than AAA

Specifications of your SpikeTidesTM

Small scale, unpurified proteotypic peptides
SpikeTidesTM with heavily labeled C-terminal lysine or arginine (Arg M +10 or Lys M +8)
SpikeTidesTM absolutely quantified using our patented Quanti-tag.
SpikeTidesTM with heavily labeled C-terminal lysine or arginine (Arg M +10 or Lys M + 8) and absolutely quantified using our patented Quanti-tag.

SpikeTidesTM  with Post-Translational Modifications
- Alkylation of cysteines (corresponding to iodoactamide method)
- Phosphorylation, glycosylation, methylation and other upon request

QC/QA for all SpikeTidesTM
ISO 9001:2008 Certification & GCLP Compliance

Selected References

•    Glatter et al.: "Large-Scale Quantitative Assessment of Different In-Solution Protein Digestion Protocols Reveals Superior Cleavage Efficiency of Tandem Lys-C/Trypsin Proteolysis over Trypsin Digestion" Journal of Proteome Research (2012)
•    Sabido et al.: "Targeted Proteomics of the Eicosanoid Biosynthetic Pathway Completes an Integrated Genomics-Proteomics-Metabolomics Picture of Cellular Metabolism" Mol Cell Proteomics (2012)
•    Hewel et al.: “Synthetic peptide arrays for pathway-level protein monitoring by LC-MS/MS.” Mol Cell Proteomics (2010)
•    Picotti et al.: "Full Dynamic Range Proteome. Analysis of S. cerevisiae by Targeted Proteomics" Cell (2009)


"My unit at the Banting and Best Department of Medical Research, University of Toronto Centre for Cellular and Biomolecular Research (CCBR) works on the systematic identification and quantification of proteins and protein complexes on a global level. In addition we have started to perform targeted proteomics to study protein regulation within pathways, e.g. signaling pathways during stem cell fate decision. For this we used JPT's various SpikeTides™ peptide products to build LC-MRM assays in a fast and cost efficient manner. More recently we also found new ways of using low cost SpikeTides™ as quantitative peptide standards in order to quantify protein targets more accurately. We are currently in the process of validating this technology for the accurate quantification of various predicted microRNA targets."
Prof. Andrew Emili (Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Canada)

“We at the ETH successfully apply JPT’s unpurified isotope- and non-labeled peptide libraries for proteome-wide SRM assays allowing us to identify and quantify proteins spanning a broad range of abundances, including proteins not previously detectable. With the ability to produce large numbers of affordable small scale proteotypic peptides we envision for the first time the development of assays for the quantitative analysis for any protein of interest and covering of whole proteomes.”
Paola Picotti, PhD (Institute of Molecular Systems Biology, ETH Zürich, Switzerland)

Product SpikeTidesTM peptides – light – heavy – quantified. The most economic source for quantified heavy peptides!
Company JPT Peptide Technologies
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JPT Peptide Technologies
Volmerstrasse 5, 12489 Berlin, Germany

Tel: +49-30-6392-7878 or 1.888.578.2660 (for USA/Canada)
Fax: +49-30-6392-7888 or 1.888.578.2666 (for USA/Canada)

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