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Measurement of L-Malic Acid in Wines Using the SpectraMax® Plus384 Microplate Reader
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Molecular Devices

Analysis of malic acid, residual sugar, volatile acidity and ammonia is very important for quality control during wine production. Enzymatic assays carried out in microplate format are quantitative and help achieve high throughput with respect to time and labor. These tests involved enzymatic conversion of the analytes to give NADH as a by-product. Measuring NADH production by measuring absorbance at 340 nm allows direct quantitation of the analytes in wine samples. Here we describe the use of MDS Analytical Technologies’ SpectraMax® Plus384 microplate reader and SoftMax® Pro software to efficiently collect and analyze data for the enzymatic determination of residual sugar in wine.

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