Corporate Banner
Satellite Banner
Proteomics
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Cellular Contamination Pathway for Heavy Elements Identified
Berkeley Lab scientists find that an iron-binding protein can transport actinides into cells.
Lemon Juice and Human Norovirus
Citric acid may prevent the highly contagious norovirus from infecting humans, scientists discovered from the German Cancer Research Center.
Signature of Microbiomes Linked to Schizophrenia
Studying microbiomes in throat may help identify causes and treatments of brain disorder.
Structural Discoveries Could Aid in Better Drug Design
Scientists have uncovered the structural details of how some proteins interact to turn two different signals into a single integrated output.
Protein Found to Play a Key Role in Blocking Pathogen Survival
Calprotectin fends off microbial invaders by limiting access to iron, an important nutrient.
Study Identifies the Off Switch for Biofilm Formation
New discovery could help prevent the formation of infectious bacterial films on hospital equipment.
How DNA ‘Proofreader’ Proteins Pick and Edit Their Reading Material
Researchers from North Carolina State University and the University of North Carolina at Chapel Hill have discovered how two important proofreader proteins know where to look for errors during DNA replication and how they work together to signal the body’s repair mechanism.
Protein Found to Control Inflammatory Response
A new Northwestern Medicine study shows that a protein called POP1 prevents severe inflammation and, potentially, diseases caused by excessive inflammatory responses.
X-ray Laser Experiment Could Help in Designing Drugs for Brain Disorders
Scientists found that when two protein structures in the brain join up, they act as an amplifier for a slight increase in calcium concentration, triggering a gunshot-like release of neurotransmitters from one neuron to another.
Team Identifies Structure of Tumor-Suppressing Protein
An international group of researchers led by Carnegie Mellon University physicists Mathias Lösche and Frank Heinrich have established the structure of an important tumor suppressing protein, PTEN.
Scroll Up
Scroll Down

MALDI-HDMSE: A Novel ‘Data Independent’ Acquisition Method for the Enhanced Analysis of 2D-Gel Tryptic Peptide Digests
Bookmark and Share

Waters Corporation

Peptide Mass Fingerprinting (PMF) is an unbiased protein identification technique that offers limited sequence information for identification purposes due to the reliance on the m/z measurement of proteolytic peptides.

An alternative technique commonly used conducts MS/MS sequencing experiments by fragmenting the tryptic peptides through sequential selection using the first analyzer of a mass spectrometer. This MS/MS approach has the following limitations:

■Only the most intense peptides are fragmented
■The user must choose the peptide to fragment
■Information is limited, as peptides can be missed if the sample is depleted
■After the sample is discarded, regaining knowledge from the sample is not feasible
■Potentially more time consuming than PMF alone

This application note proposes a novel method of acquiring data called High Definition MSE (HDMSE). This method maintains the independent nature of PMF but extends its utility by providing molecular and MS/MS-like peptide sequence information in a simple, generic fashion.

http://www.waters.com/waters/library.htm?cid=134612511&lid=134663780&xcid=x5307

Further Information

Related Content

Waters Centers of Innovation Program Honors CSU Laboratory
Proteomics and metabolomics facility recognized for innovation in biological mass spectrometry.
Wednesday, October 29, 2014
Waters Q3 Revenues up 8 Percent
Company reports third quarter sales of $493 million, an increase of 8% versus sales of $457 million in the third quarter of 2013.
Tuesday, October 21, 2014
Waters Teams With Biochemical Society on Proteomics Training Day and Symposium
The consecutive events will take place on March 24 - 27, 2014 in Chester, England.
Wednesday, November 27, 2013
Waters Acquires Nonlinear Dynamics
Nonlinear Dynamics is best known for their Progenesis software which offers researchers unique ways to analyze and visualize the raw proteomic data.
Thursday, August 08, 2013
Waters Users’ Meeting at ASMS 2013
Register for the Waters Users’ meeting at ASMS 2013 and learn about the latest developments in Mass Spectrometry from Waters experts as we reveal our new technologies and solutions.
Monday, May 13, 2013
St. John’s Research Institute Honored by Waters Center of Innovation Program
Structural Proteomics Laboratory directed by Professor Dr Amit Kumar Mandal recognized for global disease research.
Thursday, April 11, 2013
Waters Updates Alliance HPLC Designs without Impacting Established Validated Methods
The updated system will maintain key Alliance performance specifications and control algorithms to ensure that analysts can seamlessly replicate their existing methods.
Tuesday, February 26, 2013
Biological Research Laboratory at Indiana University Receives Waters Center of Innovation Honors
Mass Spec research by Professor David Clemmer has paved the way for new discoveries in proteomics and protein characterization.
Thursday, December 13, 2012
Waters Corporation Releases 2012 Sustainability Report with GRI Certification
The report provides an in-depth review of the company’s sustainability efforts within the 2011 calendar year.
Thursday, October 25, 2012
Waters and Ireland's NIBRT Partner on Training and Research
The partnership will increase large molecule characterization capacity and knowledge, helping to deliver new, more effective therapeutic options.
Thursday, September 27, 2012
Waters and Nonlinear Dynamics to Co-Develop Next Generation Research Solutions
Firms to combine complimentary expertise in analytical science and informatics to advance proteomics and metabolomics research.
Friday, August 03, 2012
Thomson Mass Spectrometry Laboratory Selected as Waters Center of Innovation
Waters recognizes Prof. Marcos Eberlin for pioneering mass spectrometry.
Tuesday, January 31, 2012
Waters Welcomes Duke University Proteomics Core Facility into Centers of Innovation Program
Arthur Moseley, Ph.D. recognized for laboratory’s research and support of biomedical research at Duke University School of Medicine.
Monday, May 16, 2011
Waters Corporation and UCLA to Convene Leading Life Scientists
Will present latest research at applications of time of flight mass spectrometry conference September 13-15.
Monday, September 06, 2010
Waters Presents Research Award to Prof. Sarah Trimpin of Wayne State University
$25,000 grant awarded at ASMS 2010 recognizes innovative mass spectrometry research and applications.
Thursday, May 27, 2010
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!