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Qualitative and Quantitative Characterization of the Metabolome, Lipidome, and Proteome of Human Hepatocytes Stably Transfected with Cytochrome P450 2E1 Using Data Independent LC/MS
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Waters Corporation

Many xenobiotics are bioactivated into toxic metabolites by cytochromes P450 (CYP). However, the activity of these enzymes typically falls in in vitro systems. Recently, a transformed human hepatocyte cell line (THLE) became available, where the metabolic activity of specific cytochromes P450 isoforms is maintained. THLE cells could be an ideal system to examine the potential toxicity of candidate pharmaceuticals. The baseline effect of the addition of CYP2E1 gene, which encodes a member of the cytochrome P450 superfamily of enzymes into THLE hepatocytes, has been characterized to better understand the biochemistry of this model system. In this application note, a label-free multi-omics approach has been applied for the analysis of the transfected human hepatocyte cell by implementing LC/HDMSE (LC-DIA-IM-MS), providing both qualitative and quantitative information within a single experiment.

A label-free multi-omics approach has been applied for the analysis of the transfected human hepatocyte cells by implementing LC/HDMSE (LC-DIA-IM-MS), providing both qualitative and quantitative information within a single experiment.

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