Corporate Banner
Satellite Banner
Proteomics
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Structure of Brain Plaques in Huntington's
Researchers at the University of Pittsburgh School of Medicine have shown that the core of the protein clumps found in the brains of people with Huntington's disease have a distinctive structure, a finding that could shed light on the molecular mechanisms underlying the neurodegenerative disorder.
Visualizing a Cancer Drug Target at Atomic Resolution
Using cryo-electron microscopy, researchers were able to view, in atomic detail, the binding of a potential small molecule drug to a key protein in cancer cells.
Pumpjack" Mechanism for Splitting and Copying DNA
High-resolution structural details of cells' DNA-replicating proteins offer new insight into how these molecular machines function
The Power of Three
Overlooked portion of cell “death receptor” critical in some cancers, autoimmune diseases.
Biomarker for Recurring HPV-Linked Oropharyngeal Cancers
A look-back analysis of HPV infection antibodies in patients treated for oropharyngeal (mouth and throat) cancers linked to HPV infection suggests at least one of the antibodies could be useful in identifying those at risk for a recurrence of the cancer, say scientists at the Johns Hopkins University.
Light Signals from Living Cells
Fluorescent protein markers delivered under high pressure.
Cellular 'Relief Valve'
A team led by scientists at The Scripps Research Institute (TSRI) has solved a long-standing mystery in cell biology by showing essentially how a key “relief-valve” in cells does its job.
Genomic Signature Shared by Five Types of Cancer
National Institutes of Health researchers have identified a striking signature in tumor DNA that occurs in five different types of cancer.
Protein Protects Against Flu in Mice
The engineered molecule doesn’t provoke inflammation and may hail a new class of antivirals.
Cat Stem Cell Therapy Gives Humans Hope
By the time Bob the cat came to the UC Davis veterinary hospital, he had used up most of his nine lives.
Scroll Up
Scroll Down

Qualitative and Quantitative Characterization of the Metabolome, Lipidome, and Proteome of Human Hepatocytes Stably Transfected with Cytochrome P450 2E1 Using Data Independent LC/MS
Bookmark and Share

Waters Corporation

Many xenobiotics are bioactivated into toxic metabolites by cytochromes P450 (CYP). However, the activity of these enzymes typically falls in in vitro systems. Recently, a transformed human hepatocyte cell line (THLE) became available, where the metabolic activity of specific cytochromes P450 isoforms is maintained. THLE cells could be an ideal system to examine the potential toxicity of candidate pharmaceuticals. The baseline effect of the addition of CYP2E1 gene, which encodes a member of the cytochrome P450 superfamily of enzymes into THLE hepatocytes, has been characterized to better understand the biochemistry of this model system. In this application note, a label-free multi-omics approach has been applied for the analysis of the transfected human hepatocyte cell by implementing LC/HDMSE (LC-DIA-IM-MS), providing both qualitative and quantitative information within a single experiment.

A label-free multi-omics approach has been applied for the analysis of the transfected human hepatocyte cells by implementing LC/HDMSE (LC-DIA-IM-MS), providing both qualitative and quantitative information within a single experiment.

http://www.waters.com/waters/library.htm?cid=134612511&lid=134686112&xcid=x5314

Further Information

Related Content

It is All About the Product, Right?
Technology Networks met with Waters Corporation at its head office in Milford, MA to better understand the factors they use to drive business success.
Monday, January 11, 2016
Waters’ Centers of Innovation Program Honors Ohio State University Laboratory
Wysocki-led facility recognized for proteomics, disease research and mass spectrometry excellence.
Wednesday, September 23, 2015
Waters Centers of Innovation Program Honors CSU Laboratory
Proteomics and metabolomics facility recognized for innovation in biological mass spectrometry.
Wednesday, October 29, 2014
Waters Q3 Revenues up 8 Percent
Company reports third quarter sales of $493 million, an increase of 8% versus sales of $457 million in the third quarter of 2013.
Tuesday, October 21, 2014
Waters Teams With Biochemical Society on Proteomics Training Day and Symposium
The consecutive events will take place on March 24 - 27, 2014 in Chester, England.
Wednesday, November 27, 2013
Waters Acquires Nonlinear Dynamics
Nonlinear Dynamics is best known for their Progenesis software which offers researchers unique ways to analyze and visualize the raw proteomic data.
Thursday, August 08, 2013
Waters Users’ Meeting at ASMS 2013
Register for the Waters Users’ meeting at ASMS 2013 and learn about the latest developments in Mass Spectrometry from Waters experts as we reveal our new technologies and solutions.
Monday, May 13, 2013
St. John’s Research Institute Honored by Waters Center of Innovation Program
Structural Proteomics Laboratory directed by Professor Dr Amit Kumar Mandal recognized for global disease research.
Thursday, April 11, 2013
Waters Updates Alliance HPLC Designs without Impacting Established Validated Methods
The updated system will maintain key Alliance performance specifications and control algorithms to ensure that analysts can seamlessly replicate their existing methods.
Tuesday, February 26, 2013
Biological Research Laboratory at Indiana University Receives Waters Center of Innovation Honors
Mass Spec research by Professor David Clemmer has paved the way for new discoveries in proteomics and protein characterization.
Thursday, December 13, 2012
Waters Corporation Releases 2012 Sustainability Report with GRI Certification
The report provides an in-depth review of the company’s sustainability efforts within the 2011 calendar year.
Thursday, October 25, 2012
Waters and Ireland's NIBRT Partner on Training and Research
The partnership will increase large molecule characterization capacity and knowledge, helping to deliver new, more effective therapeutic options.
Thursday, September 27, 2012
Waters and Nonlinear Dynamics to Co-Develop Next Generation Research Solutions
Firms to combine complimentary expertise in analytical science and informatics to advance proteomics and metabolomics research.
Friday, August 03, 2012
Thomson Mass Spectrometry Laboratory Selected as Waters Center of Innovation
Waters recognizes Prof. Marcos Eberlin for pioneering mass spectrometry.
Tuesday, January 31, 2012
Waters Welcomes Duke University Proteomics Core Facility into Centers of Innovation Program
Arthur Moseley, Ph.D. recognized for laboratory’s research and support of biomedical research at Duke University School of Medicine.
Monday, May 16, 2011
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!