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Sorting Through Cellular Statistics
Aaron Dinner, professor in chemistry, and his graduate student Herman Gudjonson are trying to read the manual of life, DNA, as part of the Dinner group’s research into bioinformatics—the application of statistics to biological research.
First Artificial Ribosome Designed
Researchers at the University of Illinois at Chicago and Northwestern University have engineered a tethered ribosome that works nearly as well as the authentic cellular component, or organelle, that produces all the proteins and enzymes within the cell.
The Genetic Roots of Adolescent Scoliosis
Scientists at the RIKEN Center for Integrative Medical Sciences in collaboration with Keio University in Japan have discovered a gene that is linked to susceptibility of Scoliosis.
HIV Susceptibility Linked to Little-Understood Immune Cell Class
High levels of diversity among immune cells called natural killer cells may strongly predispose people to infection by HIV, and may be driven by prior viral exposures, according to a new study.
New Tech Enables Epigenomic Analysis with a Mere 100 Cells
A new technology that will dramatically enhance investigations of epigenomes, the machinery that turns on and off genes and a very prominent field of study in diseases such as stem cell differentiation, inflammation and cancer has been developed by researchers at Virginia Tech.
TOPLESS Plants Provide Clues to Human Molecular Interactions
Scientists at Van Andel Research Institute have revealed an important molecular mechanism in plants that has significant similarities to certain signaling mechanisms in humans, which are closely linked to early embryonic development and to diseases such as cancer.
Toxin from Salmonid Fish has Potential to Treat Cancer
Researchers from the University of Freiburg decode molecular mechanism of fish pathogen.
Study Finds Non-Genetic Cancer Mechanism
Cancer can be caused solely by protein imbalances within cells, a study of ovarian cancer has found.
Long-sought Discovery Fills in Missing Details of Cell 'Switchboard'
A biomedical breakthrough reveals never-before-seen details of the human body’s cellular switchboard that regulates sensory and hormonal responses.
Rice Disease-Resistance Discovery Closes the Loop for Scientific Integrity
Researchers reveal how disease resistant rice detects and responds to bacterial infections.
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Tag-lite® two-cell assay: a valuable tool for protein drug discovery
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Tecan Group Ltd.

The Tag-lite technology, developed by Cisbio Bioassays, is a combination of a classical TR-FRET and SNAP-tag® technology (New England Biolabs), which allows antigens present on the cell surface to be labelled with a fluorescent donor or acceptor dye. 

The assay involves the generation of two labelled cell populations; one cell type with terbium (donor fluorophor) labelled human FcγRIIIa, and a second with surface displayed antigen Y labelled with SNAPRed (acceptor fluorophor). In the presence of an antibody which can bind both labelled proteins, the cells will come into close enough proximity to allow energy transfer between fluorophores. 

The combination of this assay system with the excellent sensitivity and flexible wavelength and bandwidth selection offered by the Infinite® M1000 PRO multimode reader provides an invaluable tool for the assay development process. This will allow more in-depth analysis of protein-protein interactions in a physiologically relevant, cell-based environment.


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