Corporate Banner
Satellite Banner
Proteomics
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Unidentified Spectra Detector
New algorithm clusters over 250 million spectra for analysis, such that millions of unidentified peptide sequences can be recognised.
New Cancer Drug Target Found in Dual-Function Protein
Findings from a study from TSRI have shown that targeting a protein called GlyRS might help to halt cancer growth.
HIV Structure Stabilized
Findings represent ‘big accomplishment’ in biomedical engineering and design.
New Cancer Drug Target in Dual-Function Protein
Scientists at The Scripps Research Institute (TSRI) have identified a protein that launches cancer growth and appears to contribute to higher mortality in breast cancer patients.
“Amazing Protein Diversity” Discovered in Maize
The genome of the corn plant – or maize, as it’s called almost everywhere except the US – “is a lot more exciting” than scientists have previously believed. So says the lead scientist in a new effort to analyze and annotate the depth of the plant’s genetic resources.
Proteins in Blood of Heart Disease Patients May Predict Adverse Events
Nine-protein test shown superior to conventional assessments of risk.
Self-Assembling Protein Shell for Drug Delivery
Made-to-order nano-cages open possibilities of shipping cargo into living cells or fashioning small chemical reactors.
Molecular Map Provides Clues To Zinc-Related Diseases
Mapping the molecular structure where medicine goes to work is a crucial step toward drug discovery against deadly diseases.
Nanoprobe Enables Measurement of Protein Dynamics in Living Cells
Mass. General and Harvard researchers use device to measure how anesthetic affects levels of Alzheimer's-associated proteins.
Scroll Up
Scroll Down

Tag-lite® two-cell assay: a valuable tool for protein drug discovery
Bookmark and Share

Tecan Group Ltd.

The Tag-lite technology, developed by Cisbio Bioassays, is a combination of a classical TR-FRET and SNAP-tag® technology (New England Biolabs), which allows antigens present on the cell surface to be labelled with a fluorescent donor or acceptor dye. 

The assay involves the generation of two labelled cell populations; one cell type with terbium (donor fluorophor) labelled human FcγRIIIa, and a second with surface displayed antigen Y labelled with SNAPRed (acceptor fluorophor). In the presence of an antibody which can bind both labelled proteins, the cells will come into close enough proximity to allow energy transfer between fluorophores. 

The combination of this assay system with the excellent sensitivity and flexible wavelength and bandwidth selection offered by the Infinite® M1000 PRO multimode reader provides an invaluable tool for the assay development process. This will allow more in-depth analysis of protein-protein interactions in a physiologically relevant, cell-based environment.


Further Information


SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!