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Analysis of a Complex Sample of Bacterial Whole Cell Lysate from Haemophilus ducreyi using the Finnigan LXQ
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Thermo Fisher Scientific

This application determines the feasibility of analyzing a complex mixture, represented by a whole cell lysate of H. ducreyi, on the LXQ linear ion trap using a simple, 1D-SDS PAGE desalting and protein fractionation procedure, and to study the effect of non-MS parameters, such as sample complexity and chromatographic resolution on the number and quality of the spectra generated and the resulting proteins identified, with particular emphasis on membrane and high molecular weight proteins.

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