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Featured Product - QuantiSir Specific Gene Knockdown Quantification Kit For Tumor Suppressors/Oncogenes
QuantiSir Specific Gene Knockdown Quantification Kit For Tumor Suppressors/Oncogenes
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Streamlining ChIP for qPCR Analysis

Webcasts
Webcast - Modifying Cell Lines for Target Discovery and ValidationModifying Cell Lines for Target Discovery and Validation
In this 30-minute seminar, we will discuss the process of genome editing, address factors affecting the design of a genome editing project, and explore the application of modified cell lines for target discovery and validation.
Webcast - Accelerate Your Research With Tecan´s New Multimode Microplate Reader Platform!Accelerate Your Research With Tecan´s New Multimode Microplate Reader Platform!
In this webinar, solutions will be presented for fast DNA quantification, single cell counting and viability analysis, and automating long-term live kinetic cell assays.

Market Reports
RNAi
RI Technologies

The report serves as a guide to global RNAi industry covering 280 plus companies that are engaged in RNAi synthesis, R&D, therapeutics, test kits, tools and services.
Innovation in the Prefilled Syringe Market - Biologics and Autoinjection Devices Shaping Future R&D Trends
Global Data

The report provides insights into possible future avenues of R&D in the prefilled syringe market. This includes an overview of problems and possible solutions, as well as discussion of how the devices have developed to date to address problems with contamination, safety, fragility and ease of use.
Monoclonal Antibodies Market in Rheumatoid Arthritis to 2018
GBI Research

The currently marketed monoclonal antibodies are highly successful products in the rheumatoid arthritis market, constituting four of the global top 20 best-selling drugs. Significant market exclusivity, expiries in the forecast period and yet more monoclonal antibodies in development will change the dynamics in and this highly lucrative market in the forecast period until 2018.
qPCR Assay Trends 2013
HTStec

HTStec's qPCR Assay Trends 2013 report was published on 15 April 2013. This 63 page market report summarizes the results of HTStec's 3rd industry-wide global web-based benchmarking survey on real-time quantitative PCR (qPCR) assays and instruments carried out in March 2013.
Circulating Tumor Cells (CTCs) and Cancer Stem Cells (CSCs) Market Report 2013
Select Biosciences Ltd

This is the latest and most up-to-date Market Report from Select Biosciences addressing the CTC and CSC markets as they are evolving rapidly.
siRNA Screening Trends
HTStec

This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on siRNA screening carried out in September 2012.
MicroRNAs and Exosomes
Select Biosciences Ltd

In this report, we focus upon the microRNA research marketplace from the perspective of the translational opportunities in these spaces. The focus of this field is moving from basic research towards the utility of associations of microRNA expression patterns in disease.
MicroRNA Market Trends 2011
Select Biosciences Ltd

In this report, we focus upon the microRNA marketplace via primary market research that allows us to identify qualitative and quantitative trends from the bottom-up.
Circulating Tumor Cells (CTCs) and Cancer Stem Cells (CSCs) Market Report 2012
Select Biosciences Ltd

In this report, we focus upon the biology of CTCs and CSCs as well as explore the inter-relationship between these cell types based upon molecular events. Also presented are research trends which are for the isolation and study of CTCs, a potentially important tool for cancer diagnostics with special relevance to cancer metastatic mechanisms.
Circulating Tumor Cells (CTCs) Market Trends 2011
Select Biosciences Ltd

This is the latest and most up-to-date Market Report from Select Biosciences addressing the circulating tumor cells (CTCs) space. In this report, we focus upon the emerging marketplace for the isolation and study of CTCs, a potentially important tool for cancer diagnostics with special relevance to cancer metastasis and mechanisms thereof.
Scientific News
Chemical Tag Marks Future microRNAs for Processing
New research reveals how cells sort out the loops meant to encode small RNAs.
Blood Test Could Help Bowel Cancer Patients Avoid Drug Side-Effects
Manchester researchers have provided early evidence to suggest that a blood test could be used to identify bowel cancer patients that may benefit from more intensive chemotherapy.
Bright New Hope for Beating Deadly Hereditary Stomach and Breast Cancers
Researchers use genomic screening to search for vulnerabilities in the cancer cells.
Novel Approach Gives Insights Into Tumor Development
Scientists used a powerful new technique to turn off all the genes in mouse lung cancer cells and test how they affect tumor growth and metastasis.
New Cyclotron Facility at UT Southwestern
Expands research opportunities and imaging capabilities for detecting, tracking cancer.
Strengthening the Immune System’s Fight Against Brain Cancer
NIH-funded research suggests novel way to improve vaccine efficacy in brain tumors.
World-First Cancer Drugs Could Work In Larger Group Of Patients
A pioneering class of drugs that target cancers with mutations in the BRCA breast cancer genes could also work against tumours with another type of genetic fault, a new study suggests.
Combination of CBD and THC Cannabinoids Inhibit Glioma Brain Cancer Growth
Study published in the journal Molecular Cancer Therapeutics.
New Approach to Promote Regeneration of Heart Tissue
Study in animal model paving way forward for tissue repair.
New Gene Therapy For Hemophilia Shows Potential As Safe Treatment
Research showed that bleeding events were drastically decreased in animals with hemophilia B. Using a viral vector to swap out faulty genes proved safe and could be used for the more common hemophilia A.
Business News
Kite Pharma Strengthens its TCR Cancer Gene Therapy Platform
Kite to expand product pipeline in TCRs for the treatment of solid tumors, complementing Kite's Chimeric Antigen Receptor pipeline.
WuXi PharmaTech to Build New Cell Therapy Manufacturing Facility
Facility is designed for CAR T cells and other cancer immunotherapies.
QIAGEN Expands Leadership in Liquid Biopsies
QIAGEN and Tokai Pharmaceuticals to co-develop and commercialize the first regulated CTC-based companion diagnostic to guide treatments in prostate cancer.
Horizon to Support Cancer Research UK’s Stratified Medicine Program
Horizon’s Diagnostics division to provide genetically defined, human genomic reference standards.
First Treatment Approved Under EAMS
New immunotherapy (pembrolizumab) made available in the UK for treatment of adults with advanced melanoma.
WGBS Announces Research Collaboration with Leading Canadian Laboratory
Goal of enabling affordable, large-scale BRCA1 and BRCA2 screening for general population.
ModiQuest Research and Cellectis to Collaborate
Collaboration on antibody generation for potential CAR development.
Arrowhead Acquires Novartis' RNAi R&D Portfolio
The acquisition includes multiple patent families covering RNAi-trigger design rules and modifications.
Regulatory Submission for Phase 2b Trial for Novel AML Consolidation Treatment
BL-8040 Phase 2b study, as consolidation treatment for AML patients responding to standard induction treatment, is expected to commence shortly after receipt of regulatory approval, anticipated in next few months.
BIOTECHNICA, LABVOLUTION and ELA: Strong Trio in October
Deutsche Messe AG to launch the new trade fair from 6 to 8 October this year.

Events
Personalised Medicine
12 May 2015
Personalised Medicine ...
More information
BIT
15 May 2015
BIT's 8th Annual World Cancer ...
More information
ADC Summit 2015
18 May 2015
ADC Summit 2015 ...
More information
Targeting Cancer Metabolism
26 May 2015
Targeting Cancer Metabolism ...
More information

ePosters
Improved Small RNA Library Preparation Workflow for Next-Generation Sequencing
TriLink BioTechnologies

We describe an optimized small RNA NGS library prep workflow using chemically modified adapters which suppresses adapter dimers, allows for RNA inputs down to 1 ng and eliminates the need for a gel purification step, thus allowing full automation not previously possible.
Specificity of highly potent miRNA inhibitors


miRNA inhibitors are invaluable tools for elucidating the roles of miRNAs. However, potent inhibitors may also affect other miRNAs. To understand the potential cross-reactivity of miRNA inhibitors, various miRNA inhibitor designs were systematically tested. We demonstrate that mismatches both within and outside the seed region of the miRNA interfere with inhibition. Our findings indicate that features important for natural miRNA target recognition are also important for inhibitor specificity.
Targeting Cancer Stem Cell-Related miRNAs for Prostate Cancer Therapy
SYMBIOSIS SCHOOL OF BIOMEDICAL SCIENCES, SYMBIOSIS INTERNATIONAL UNIVERSITY, LAVALE, PUNE - 412115

The poster focuses on the pivotal function of miRNAs in tumorigenesis by regulation of self renewal and apoptosis via cancer stem cell signalling pathways with special focus on their regulation of Epithelial to Mesenchymal transition in metastatic prostate cancer.

siRNA Screening: Development of Hit Stratification Strategies


This poster compares different approaches to hit stratification and validation after an initial screen. Standard siRNA reagents deconvoluted from a pooled set of four were compared to a pooled set of four specificity enhanced reagents. High confidence hits were similar. To explore the validity of low confidence hits, a chimeric approach was used whereby a gene-specific seed sequence was introduced into a non-targeting siRNA scaffold. This work resulted in new hit stratification strategies.
Identification of microRNA targets using microRNA modulation techniques and gene expression arrays


By examining the overlap of messages down-regulated by miRNA mimics and up-regulated by miRNA inhibitors, we robustly identify miRNA-regulated messages, many of which have canonical seed matches and some which are not identied by standard target prediction programs.
Specificity and functionality of microRNA inhibitors


Our findings indicate that features important for natural miRNA target recognition also appear to be important for inhibitor specificity. Understanding the specificity of inhibitors allows for better interpretation of inhibitor activity in endogenous systems.
Alternative miRNA design for therapeutic RNAi applications


For in vivo applications, the design of miRNA inhibitors and miRNA mimics must be optimized for stability and potency. However, stabilized miRNA mimic molecules can lose functionality compared to standard miRNA mimic molecules due, in part, to the activity of the stabilized passenger strand acting as a miRNA inhibitor. We discuss how mismatches affect the activity of the stabilized miRNA mimics, perhaps by generating a passenger strand that is less functional as an inhibitor molecule.
Integrating Fluorescent Carbon Nanodot Synthesis and Optical Detection of Methylmercury
University of Vigo (Spain)

In the last years, a great interest toward development of optical nanoprobes has arisen, so fluorescent nanomaterials have been implemented in analytical systems for the detection of several species. In this work, a novel assay that integrates the synthesis of fluorescent carbon dots (CDs) and sensing within one step, for the fast, sensitive and selective detection of methylmercury is presented.
Impact of Molecular Surface Charge on Electrical Impedance Spectroscopy Biosensing
Tel Aviv University

Molecular surface charge was found to be the dominant parameter when monitoring protein binding events by Electrical Impedance Spectroscopy with a charged redox couple. A biosensing device was fabricated, and a physical model was derived to explain the results.
A multiplexed amplicon sequencing technology for FFPE and circulating, cell-free DNA
Swift Biosceinces

A novel amplicon approach allowing for hundreds of amplicons to be multiplexed in a single tube with a two workflow from sample to sequencer.
Application Notes
Assessing Drug Encapsulation Efficiency using Nanoparticle Tracking Analysis
Malvern Instruments Limited

In this application note we describe the use of Nanoparticle Tracking Analysis (NTA) for size and concentration measurements of drug delivery nanoparticles. In addition, by using a fluorescently tagged drug molecule, it was possible to determine how many drug delivery nanoparticles had successfully been loaded with drug molecules.
Creating dose-response curves for cell-based and biochemical assays with the HP D300 Digital Dispenser
Tecan Group Ltd.

Small molecule drug discovery relies on the evaluation of one compound against another compound. Measurement of the activity of the small molecule against a target must be standardized by determining the IC50 or EC50 value using does response curve. The classical method of generating a dose-response curve is via serial dilution; however this approach has a number of drawbacks. Today, there is an alternative solution which eliminates these limitations: the HP D300 Digital Dispenser.
3D Cell Culture: mimicking real tissue
Tecan Group Ltd.

Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory. Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
Optimized cell culture
Tecan Group Ltd.

Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.
Automated Illumina NGS Sample Preparation: TruSeq® Stranded mRNA on the Hamilton Microlab STAR
Hamilton Robotics

Illumina’s sample preparation kits produce high quality libraries for DNA and RNA sequencing. Hamilton offers the robust Microlab STAR line of robotic liquid handling workstations for automating sample preparation. Together, the automation of the TruSeq Stranded mRNA sample preparation protocol on the STAR gives the user hours of reliable, unattended library preparation.
Automating Aqueous Compound Solubility Using the 96-Well Multiscreen® Solubility Filter Plate on a Hamilton Microlab® Star Workstation
Hamilton

Here we describe an automated method for determining drug solubility using Millipore’s MultiScreen Solubility filter plate on the Hamilton MICROLAB STAR workstation.
A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1)
BMG LABTECH

Post-translational histone modifications like acetylation play a pivotal role in the epigenetic regulation of transcription. Catalyzing the latter reaction HDACs affect various cellular processes especially cancerogenesis. Although the mechanism of starting cancerogenesis by epigenetic events is not clearly explained inhibition of HDACs has highlighted as a viable principle in cancer therapy.
Dual-Glo Luciferase Reporter-Gene Assay on the SpectraMax M5, LMax II384 and Analyst GT Microplate Readers
Molecular Devices

Reporter-gene assays are used to study eukaryotic gene expression. Dual genetic reporters are commonly used in transient transfections of cultured cells to minimize experimental variability. Recently, Promega introduced a Dual-Glo Lucoferase assay system which replaced the outdated ‘flash’ assays used previously. Ensuring both genetic reporters have different substrate requirements enables them to be detected using a single reaction mixture.
Sequence-Specific DNA Assay
Molecular Devices

The Threshold® system from Molecular Devices, together with the Immuno Ligand Assay (ILA) kit can be used to measure specific DNA sequences. Biotinylated or fluorescienated oligonucleotide probes, specific to the target sequence, anneal to the digested target DNA creating a probe-target hybrid which can then be detected and quantified against a standard curve, with pleasing results.
Measurement of Proteasome Inhibition in Live Cells in Molecular Devices Microplate Fluorometers
Molecular Devices

The proteasome is a massive protein complex which can recognize and bind targeted proteins, and inject them into the central core where the proteins are successively degraded into short peptides. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.
SELECTBIO

SELECTBIO Market Reports
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