Corporate Banner
Satellite Banner
RNAi
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

New Genomic Technique Uncovers Coral Transcriptome

Published: Tuesday, May 12, 2009
Last Updated: Tuesday, May 12, 2009
Bookmark and Share
Researchers have uncovered the larval transcriptome of a reef-building coral by utilizing a new technique for cDNA preparation.

Using a new technique for cDNA preparation combined with the latest sequencing methods, researchers have uncovered the larval transcriptome of a reef-building coral (Acropora millepora). Their study, described in the open access journal BMC Genomics, features the most extensive database of genes and genetic markers currently available for any coral.

A collaborative team of researchers from the University of Texas at Austin and Indiana University, led by Mikhail Matz and John Colbourne, developed a new method for transcriptome analysis based on next-generation 454 sequencing technology.

According to Eli Meyer, the primary author on the paper, “Our method specifically addresses problems that had hindered the sequencing of transcriptomes with 454, such as homopolymer (A/T) tracks, adaptor artifacts, and uneven transcript coverage”.

The new method requires as little as 1 microgram of total RNA, eliminates the need for a strand-selection step prior to emulsion PCR, and results in 2-5 fold greater 454 sequence output than in any previously reported attempts.  Meyer said, “These tools allow for the rapid development of sequence resources for any organism at a low cost – a few weeks and a few thousand dollars”.

The protocol outlined in the paper was developed for 454-FLX. The current version of the protocol (available from the authors' website) is already modified and tested to work with the latest ‘Titanium’ version of 454.

As well as demonstrating the technical power of the method, the study has identified approximately 11,000 genes on the basis of sequence similarity with known proteins, over 30,000 markers of genetic variation, and a number of novel candidate genes for stress-related processes.  The characterization of the larval transcriptome for this widely studied coral will enable research into the biological processes underlying stress responses in corals and their evolutionary adaptation to global climate change.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Gene-Editing Halts DMD Progression
Using a new gene-editing technique, a team of scientists from UT Southwestern Medical Center stopped progression of Duchenne muscular dystrophy (DMD) in young mice.
Tuesday, January 05, 2016
Researchers Develop Vaccine that Protects Primates Against Ebola
A collaborative team from The University of Texas Medical Branch at Galveston and the National Institutes of Health have developed an inhalable vaccine that protects primates against Ebola.
Thursday, July 23, 2015
Can Cell Cycle Protein Prevent or Kill Breast Cancer Tumors?
An MD Anderson study has shown the potential of a simple molecule involved in cancer metabolism as a powerful therapeutic.
Monday, July 20, 2015
Cancer-Causing Virus Blocks Human Immune Response
Epstein-Barr virus shown to outwit the human immune response using microRNAs.
Wednesday, January 28, 2015
Metabolic Protein Launches Sugar Feast that Nurtures Brain Tumors
PKM2 slips into nucleus to promote cancer; potential biomarker and drug approach discovered.
Wednesday, November 28, 2012
Ancient Enzymes Function like Nanopistons to Unwind RNA
Molecular biologists have solved one of the mysteries of how double-stranded RNA is remodeled inside cells in both their normal and disease states.
Wednesday, September 05, 2012
Good Cholesterol' Nanoparticles Seek and Destroy Cancer Cells
Nanoparticles loaded with small interfering RNA to silence cancer-promoting genes selectively shrunk or destroyed ovarian cancer tumors in mice.
Thursday, May 05, 2011
'Good Cholesterol' Nanoparticles Seek and Destroy Cancer Cells
Scientists package HDL with gene-silencing siRNA to target tumors, spare normal tissue.
Wednesday, April 13, 2011
Scientific News
Counting Cancer-busting Oxygen Molecules
Researchers from the Centre for Nanoscale BioPhotonics (CNBP), an Australian Research Centre of Excellence, have shown that nanoparticles used in combination with X-rays, are a viable method for killing cancer cells deep within the living body.
Crowdfunding the Fight Against Cancer
From budding social causes to groundbreaking businesses to the next big band, crowdfunding has helped connect countless worthy projects with like-minded people willing to support their efforts, even in small ways. But could crowdfunding help fight cancer?
Cancer Cells Kill Off Healthy Neighbours
Cancer cells create space to grow by killing off surrounding healthy cells, according to UK researchers working with fruit flies.
Cancer Drug Target Visualized at Atomic Resolution
New study using cryo-electron microscopy shows how potential drugs could inhibit cancer.
Genetic Mechanism Behind Cancer-Causing Mutations
Researchers at Indiana University has identified a genetic mechanism that is likely to drive mutations that can lead to cancer.
Future of Medicine Could be Found in a Tiny Crystal Ball
A Drexel University materials scientist has discovered a way to grow a crystal ball in a lab. Not the kind that soothsayers use to predict the future, but a microscopic version that could be used to encapsulate medication in a way that would allow it to deliver its curative payload more effectively inside the body.
"Gene Fusion" Drives Childhood Brain Cancers
Study co-led by Penn scientists highlights potential targets for future cancer therapies.
Enzyme Links Age-Related Inflammation, Cancer
Researchers have shown that an enzyme key to regulating gene expression -- and also an oncogene when mutated -- is critical for the expression of numerous inflammatory compounds that have been implicated in age-related increases in cancer and tissue degeneration.
Viral Gene Editing System Corrects Genetic Liver Disease
Penn study has implications for developing safe therapies for an array of rare diseases via new gene cut-and-paste methods.
Improving Delivery of Poorly Soluble Drugs Using Nanoparticles
A technology that could forever change the delivery of drugs is undergoing evaluation by the Technology Evaluation Consortium™ (TEC). Developed by researchers at Northeastern University, the technology is capable of creating nanoparticle structures that could deliver drugs into the bloodstream orally – despite the fact that they are normally poorly soluble.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!