Corporate Banner
Satellite Banner
RNAi
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

New Portable Device Enables RNA Detection from Ultra-Small Sample

Published: Friday, November 09, 2012
Last Updated: Friday, November 09, 2012
Bookmark and Share
A new power-free microfluidic chip developed by researchers at the RIKEN Advanced Science Institute (ASI) enables detection of microRNA from extremely small sample volume in only 20 minutes.

By drastically reducing the time and quantity of sample required for detection, the chip lays the groundwork for early-stage point-of-care diagnosis of diseases such as cancer and Alzheimer's.

MicroRNAs (miRNAs) are small, non-coding RNA molecules that regulate gene expression in a wide range of biological processes including development, cell proliferation, differentiation and cell death (apoptosis). Concentration of certain miRNA in body fluids increases with the progression of diseases such as cancer and Alzheimer's, generating hope that these short RNA may hold the key to faster, more accurate diagnosis. Currently available techniques for sensitive miRNA detection, however, require days to reach a diagnosis and involve equipment operated only by trained personnel, making them impractical for use in many situations.

The research team set out to overcome these obstacles by developing a device that enables fast, easy-to-use point-of-care (POC) diagnosis from only a very small sample. In earlier research, the team developed a device in the form of a microchip which uses polydimethylsiloxane (PDMS), a silicone compound known for its air absorption properties, to pull reagents into a capture probe for analysis. This pumping technique simplified design by eliminating the need for external power sources, but the device required a quantity of sample too large for practical applications.

The new device also uses PDMS as an air pump, but drastically improves the method's sensitivity through a signal amplification method called laminar flow-assisted dendritic amplification (LFDA). First, DNA fragments which bond to specific miRNA sequences are fixed to a glass surface along with the miRNA sample to be analyzed, and then sandwiched under a layer of PDMS with channels in it (Figure 1). Emptied of air in a vacuum, the PDMS layer induces a pump effect which pulls amplification reagents, inserted at the channel inlets, into the channels and into contact with the miRNA, creating fluorescence-labeled dendritic structures that grow over time and can be quickly detected.

The sensitivity of this technique drastically reduces the sample quantity required for diagnosis to only 0.25 attomoles (10-18 mole), a thousand-fold improvement over the team's earlier model. Together with its detection time of only 20 minutes, these properties make the self-powered device ideal for use in resource-poor environments, promising portable point-of-care diagnosis for millions in developing countries and around the world.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Genetic Variant Linked to Development of Liver Cancer in Hepatitis C Virus Carriers
The research group conduct a genome-wide study to identify risk factors connecting HVC and HCC.
Monday, July 04, 2011
Scientific News
Liquid Biopsies: Utilization of Circulating Biomarkers for Minimally Invasive Diagnostics Development
Market Trends in Biofluid-based Liquid Biopsies: Deploying Circulating Biomarkers in the Clinic. Enal Razvi, Ph.D., Managing Director, Select Biosciences, Inc.
Self-Assembling, Biomimetic Membranes May Aid Water Filtration
A synthetic membrane that self assembles and is easily produced may lead to better gas separation, water purification, drug delivery and DNA recognition, according to an international team of researchers.
Error Correction Mechanism in Cell Division
Cell biologists have reported an advance in understanding the workings of an error correction mechanism that helps cells detect and correct mistakes in cell division early enough to prevent chromosome mis-segregation and aneuploidy, that is, having too many or too few chromosomes.
Researchers Resurrect Ancient Viruses
Researchers at Massachusetts Eye and Ear and Schepens Eye Research Institute have reconstructed an ancient virus that is highly effective at delivering gene therapies to the liver, muscle, and retina.
Cell Aging Slowed by Putting Brakes on Noisy Transcription
Experiments in yeast hint at ways to extend life of some human cells.
Crucial for Stem Cell Survival Protein Identified Using Editing Tool CRISPR
A team of University of Wisconsin-Madison engineers has identified a protein that is integral to the survival and self-renewal processes of human pluripotent stem cells (hPSC).
Long Telomeres Associated with Increased Lung Cancer Risk
Genetic predisposition for long telomeres predicts increased lung adenocarcinoma risk.
First Artificial Ribosome Designed
Researchers at the University of Illinois at Chicago and Northwestern University have engineered a tethered ribosome that works nearly as well as the authentic cellular component, or organelle, that produces all the proteins and enzymes within the cell.
Identifying a Key Growth Factor in Cell Proliferation
Researchers discover that aspartate is a limiter of cell proliferation.
A Gene-Sequence Swap Using CRISPR to Cure Haemophilia
For the first time chromosomal defects responsible for hemophilia have been corrected in patient-specific iPSCs using CRISPR-Cas9 nucleases
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!