The new kit enables researchers to prepare high-quality, strand-specific libraries for next-generation sequencing. The ability to gain valuable strand-specific information from RNA-sequencing experiments allows researchers to more easily discern overlapping transcripts and investigate antisense expression for greater understanding of gene regulation.
The kit has been optimized to provide outstanding sequencing performance and uniform coverage of the entire transcript. This is achieved through greater library complexity and less 5'/3' bias, even with small amounts of starting material (as low as 50 ng). The kit has also been designed to be as complete as possible, including master-mixed reagents and a streamlined workflow for less hands-on time and faster results.
"Strand-specific RNA-sequencing library preparation is the latest addition to Agilent's next-generation sequencing/gene-regulation portfolio, which offers the most complete integrated solutions from sample to analysis," said Yong Yi, Agilent associate director of marketing for genomics. "We are thrilled to provide another highly sensitive tool that will advance understanding of how gene regulation governs normal and aberrant biological processes."
Other key products in Agilent's gene-regulation portfolio include SureSelect human methyl-seq, targeted RNA-seq, gene expression and miRNA microarrays, GeneSpring data analysis software, 2100 Bioanalyzer, and 2200 TapeStation.