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Cambio now supplies UK life scientists with the new Globin-Zero™ Gold Kit from Epicentre. This innovative product is used to clean up excess globin mRNA and rRNA in total blood RNA samples, allowing improved identification and sequencing of the target RNA components.
Globin-Zero™ Gold Kits efficiently remove abundant globin RNAs from both intact and degraded human, mouse and rat total blood RNA samples.
In these samples, as much as 70% of the mRNA can be globin mRNA, and this would account for a large majority of sequencing reads in RNA-Seq experiments.
By removing excess RNA, the Globin-Zero™ Gold Kit enables deeper sequencing into blood RNA samples and improved identification of less abundant RNA types, including viral RNA.
Complete in just 90 minutes, the rapid, single-pass process saves time and reduces sample waste compared to common multi-step procedures, which require the use of various kits to complete the same task.
Using the Globin-Zero™ Gold Kit, researchers can collect even more information from their investigations by retaining non-globin mRNA and large non-ribosomal, non-coding RNA for whole transcriptome RNA-Sequencing.
The magnetic bead format means that the technique can be used with both manual and automated processing, further reducing the hands-on time required.
