Satellite Banner
RNAi
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

3D Spheroid Assays for Cancer Drug Screening

Published: Thursday, June 27, 2013
Last Updated: Thursday, June 27, 2013
Bookmark and Share
New tool to allow cell-based assays to be carried out in 3D.

AMSBIO has introduced a new range of 96-well format 3D Spheroid Cell Proliferation / Viability Assays, providing a new tool to allow cell-based assays to be carried out in 3D.

The new AMSBIO 3D Spheroid Proliferation/Viability Assay provides a useful tool for modeling tumor response in vitro.

The kit utilizes a 3D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation Extracellular Matrix to drive aggregation and/or spheroid formation of cells.

Upon completion of spheroid formation, the spheroid may be treated with pharmacological agents to evaluate tumor viability after drug treatment.

Tumor spheroid expansion is visualized microscopically and can be quantitated through image analysis software for real-time and label free evaluation.

At the conclusion of the assay, cell viability may be assessed by fluorescence using Resazurin.

The 3D Spheroid Fluorometric Proliferation/Viability Assay offers an in vitro, standardized, three-dimensional, high content format for inducing multicellular tumor spheroid (MCTS) formation and quantitating cell viability within the spheroids in response to pharmacological treatment.

The AMSBIO 3D Spheroid Proliferation/Viability Assay establishes physiological gradients for nutrients, oxygen, catabolites, and pH due to limitations in diffusion through multicellular layers.

These gradients also promote the creation of heterogeneous cell populations with proliferating cells on the spheroid surface, quiescent cells in the intermediate layers, and necrotic cells in the core, similar to an avascular tumor.

Uniform spheroid size and physiology is determined through cell seeding, providing a robust and reproducible assay format for drug screening or pathway analysis.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More than 4,900+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Webinar Discusses DNA Damage in Individual Cells
New on-demand webinar from AMSBIO.
Monday, September 30, 2013
Informative Guide to Gene Silencing & Delivery
AMSBIO’s new guide - 'Top Ten Tips for Gene Silencing & Delivery'.
Tuesday, June 19, 2012
Scientific News
Gene Therapy for Metabolic Liver Diseases
Researchers have tested gene therapy in pigs from hereditary tyrosinemia type 1, with corrected liver cells being transplanted into the diseased liver.
Gene Terapy for Muscle Wasting Developed
New gene therapy could save millions of people suffering from muscle wasting disease.
Testing Nanoparticle Drug Delivery in Dogs
Scientists have tested a nanoparticle drug delivery against bone cancer in dogs with promising results.
Fighting Cancer Through Protein Pathways
Researchers have found a new drug target within a protein production pathway critical to regulating growth and proliferation of cells.
Triple-Action Therapy Patch Shows Promise
Patch that delivers drug, gene, and light-based therapy to tumor sites shows promising results in mice.
Cancer Gene-Drug Combinations Ripe for Precision Medicine
The study aims to expand the number of cancer gene mutations that can be paired with a precision therapy.
Exploiting Malaria’s Achilles’ Heel
Researchers have uncovered an Achilles' heel in malaria's anti-drug treatment arsenal that could lead to a disease cure.
Targeting BRAF Mutations in Thyroid Cancer
Treating metastatic thyroid cancer patients harboring a BRAF mutation with vemurafenib showed anti-tumor activity in a third of patients.
Colon Cancer Blocked in Mice
Case Western Reserve University Researchers block common type of colon cancer tumour in mice, laying groundwork for human clinical trial.
Protein Nanocages Could Improve Drug Design and Delivery
HHMI scientists have designed and built 10 large protein icosahedra that are similar to viral capsids that carry viral DNA.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!