Corporate Banner
Satellite Banner
RNAi
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Roche Launches GS FLX+ System Software Update

Published: Wednesday, August 07, 2013
Last Updated: Wednesday, August 07, 2013
Bookmark and Share
The latest software version 2.9 enables highly accurate sequencing of long 16S ribosomal RNA amplicons for more precise taxonomic assignment of organisms in microbial surveys.

Roche has announced the global launch of a new software update that significantly extends the range of amplicon lengths available for targeted next-generation sequencing studies on the 454 GS FLX+ System.

The latest software enables sequencing of amplicons up to 800 bp while maintaining >99% accuracy over the length of the read.

High quality sequencing of even longer amplicons up to 1,100 bp has been successfully demonstrated in early access testing.

The long read improvements allow researchers to obtain more complete coverage of target gene regions using fewer amplicons, both simplifying experimental design and improving the biological relevance of sequencing results.

“This is another step in our continued efforts to drive high quality, long read sequencing advancements for the genomics community,” said Frank Pitzer, President Roche Sequencing Solutions. “We are actively working to make these same long read developments available on our benchtop GS Junior System in 2014.”

Long amplicon read lengths are critical for a variety of targeted sequencing applications including human gene sequencing and viral drug resistance studies as well as microbial community analysis.

Early results of targeted sequencing of 16S and 18S ribosomal RNA genes to accurately profile mixed communities have shown that the extended amplicon read lengths allow coverage of more variable regions in a single read.

This results in more precise taxonomic assignment of organisms down to the genus and even species level, which is of particular importance for microbiome studies in clinical research.

Early access testing of the latest GS FLX+ software v2.9 was led by prominent researchers in the metagenomics field who also have optimized popular open source microbial community profiling tools QIIME and mothur for the latest long read data.

“The new software for the GS FLX+ System will enable researchers to obtain considerably longer sequence reads from 16S rRNA gene amplicons without sacrificing data quality,” explained Patrick Schloss, PhD, Assistant Professor at the University of Michigan and author of mothur.

Schloss continued, “This is a great advance for microbial ecologists since it will enable them to get better taxonomic classification of their data.”

In addition to advancing microbial profiling studies, the new GS FLX+ long amplicons will allow more comprehensive detection of genetic variations in human gene panels by spanning more introns and exons.

The long reads also improve haplotype phasing of more distantly linked variants, which is critical for genotyping of highly polymorphic regions of the genome such as the Human Leukocyte Antigen (HLA) gene.

Longer amplicon read lengths also significantly ease sequencing experimental design, workflow, and analysis by allowing optimal primer placement, the use of fewer amplicons to cover target regions, and direct transition of pre-existing Sanger amplicons to the GS FLX+ System.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,400+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

FDA Approves Roche’s Advanced Cervical Cancer Chemotherapy
Avastin is the first biologic medicine approved in combination with chemotherapy to help women with this type of cancer live longer than with chemotherapy alone.
Monday, August 18, 2014
Roche to Acquire Seragon Pharmaceuticals
Acquisition of Phase I program offers a potential new approach for hormone receptor-positive breast cancer.
Wednesday, July 02, 2014
Roche Recieves FDA Approval for Cervical Cancer Screening HPV Test
Expanded indication makes cobas HPV Test the only test approved in U.S. that can be used instead of Pap in first-line primary screening in women 25 and older.
Friday, April 25, 2014
Roche, Oryzon Announce Epigenetics Collaboration
Collaboration spans research, development and commercialization of LSD1 inhibitors for oncology, haematology and non-malignant conditions.
Monday, April 07, 2014
FDA Recommends Roche's HPV Test as Primary Screening Tool
Test will assess risk of cervical cancer of women 25 years and older.
Thursday, March 13, 2014
Roche and Molecular Partners Enter into Alliance
Partnership will combine novel biologics with Roche drug-conjugate technology to develop new cancer treatments.
Wednesday, December 04, 2013
FDA Approves New Streamlined Workflow Option for Roche's Cobas HPV Test
New process allows labs to use same Pap test vial instead of transferring sample to new vial, thus simplifying workflow.
Friday, June 28, 2013
Roche and Isis Pharmaceuticals Form Alliance for Huntington's Disease
This alliance combines Isis' antisense expertise with Roche's scientific expertise in developing neurodegenerative therapeutics.
Wednesday, April 10, 2013
Roche 454 Sequencing Systems Successfully Resolve Genetic Mutations in Blood Cancer Cases
International study presented at ASH Meeting demonstrates the potential of 454 Sequencing Systems to comprehensively characterize an individual’s blood cancer type to guide personalized therapy decisions.
Thursday, December 13, 2012
Is Lunar Dust Harmful to Humans?
Exposure to lunar dust during the Apollo missions resulted in reports of ocular, respiratory and dermal irritation, suggesting that lunar dust is a potential risk to human health.
Sunday, April 17, 2011
Roche’s Assay for 2009 H1N1 Influenza Virus is Regularly Updated with the Latest Virus Sequences
The assay has been developed to provide a rapid means of identification of infected patients.
Thursday, December 03, 2009
Roche’s xCELLigence System for Determination of Optimal Time Points for Gene and Protein Expression Analysis
The xCELLigence RTCA System from Roche Applied Science allows for label-free, continuous monitoring of cell phenotypic changes using electrical impedance as readout. The interaction of cells with the electronic biosensors leads to the generation of a cell-electrode impedance response which indicates the status of the cells in terms of cell number, cell viability, cell morphology and cell attachment quality. Real-time, continuous measurement ensures the documentation of cell phenotypes in the fo
Friday, August 14, 2009
Scientific News
Long Telomeres Associated with Increased Lung Cancer Risk
Genetic predisposition for long telomeres predicts increased lung adenocarcinoma risk.
First Artificial Ribosome Designed
Researchers at the University of Illinois at Chicago and Northwestern University have engineered a tethered ribosome that works nearly as well as the authentic cellular component, or organelle, that produces all the proteins and enzymes within the cell.
Identifying a Key Growth Factor in Cell Proliferation
Researchers discover that aspartate is a limiter of cell proliferation.
A Gene-Sequence Swap Using CRISPR to Cure Haemophilia
For the first time chromosomal defects responsible for hemophilia have been corrected in patient-specific iPSCs using CRISPR-Cas9 nucleases
New Tool Uses 'Drug Spillover' to Match Cancer Patients with Treatments
Researchers have developed a new tool that improves the ability to match drugs to disease: the Kinase Addiction Ranker (KAR) predicts what genetics are truly driving the cancer in any population of cells and chooses the best "kinase inhibitor" to silence these dangerous genetic causes of disease.
New Material Opens Possibilities for Super-Long-Acting Pills
A pH-responsive polymer gel could create swallow able devices, including capsules for ultra-long drug delivery.
New Tool For Investigating RNA Gone Awry
A new technology – called “Sticky-flares” – developed by nanomedicine experts at Northwestern University offers the first real-time method to track and observe the dynamics of RNA distribution as it is transported inside living cells.
Access Denied: Leukemia Thwarted by Cutting Off Link to Environmental Support
A new study reveals a protein’s critical – and previously unknown -- role in the development and progression of acute myeloid leukemia (AML), a fast-growing and extremely difficult-to-treat blood cancer.
New Weapon in the Fight Against Blood Cancer
This strategy, which uses patients’ own immune cells, genetically engineered to target tumors, has shown significant success against multiple myeloma, a cancer of the plasma cells that is largely incurable.
TOPLESS Plants Provide Clues to Human Molecular Interactions
Scientists at Van Andel Research Institute have revealed an important molecular mechanism in plants that has significant similarities to certain signaling mechanisms in humans, which are closely linked to early embryonic development and to diseases such as cancer.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!