Corporate Banner
Satellite Banner
RNAi
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Automated TruSeq® Stranded Total RNA Library Preparation Method

Published: Tuesday, February 25, 2014
Last Updated: Tuesday, February 25, 2014
Bookmark and Share
Stranded Total RNA sequencing library preparation using epMotion minimizes hands-on time.

Eppendorf has announced the release of the epMotion automation method for Illumina’s TruSeq Stranded Total RNA library preparation kit for next-generation sequencing as “Illumina Qualified.”

“Illumina Qualified” indicates that Illumina’s analysis of libraries prepared with this epMotion method has shown the libraries to perform comparably to those prepared manually.

Sample preparation for next-generation sequencing (NGS) involves extensive manual processing that can affect reproducibility. Eppendorf epMotion Liquid Handling systems are important tools for labs that want to significantly increase throughput and consistency of results.

The automation of labor-intensive steps in NGS sample prep, like pipetting, mixing, temperature control and magnetic separation, brings about a standardized and streamlined process, with significantly reduced hands-on time and minimization of human errors.

“Eppendorf’s method for TruSeq Stranded Total RNA provides our customers with another great option for high throughput automation,” said Peter Fromen, Senior Director of Product Marketing at Illumina. “Eppendorf is one of the leading providers of automation to NGS users, and we are looking forward to additional methods from them in the near future.”

“The Stranded Total RNA sequencing library preparation using the epMotion minimizes our hands-on time while achieving overall high reproducibility. This streamlines and standardizes our sequencing workflow, thereby allowing for higher sequencing throughput. We are looking forward to collaborating with Eppendorf on additional automated library prep protocols,” said Dr. Wei Chen, Head of the Scientific Genomics Platform at Max Delbrück Centre for Molecular Medicine in Berlin, Germany.

“This method is tailored for 8, 16 or 24 samples of 100 to 1000 ng total RNA and gives sequencing ready libraries in 9 to 12 hours. While this is not much faster than the manual process, the hands-on time is significantly reduced as the automated process needs less than one hour preparation time in total,” said Dr. Reza Hashemi, Executive Director of Portfolio Management Liquid Handling at Eppendorf.

Additional automated methods for NGS library preparation from Illumina are currently in development.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Turning Skin Cells into Heart, Brain Cells
In a major breakthrough, scientists at the Gladstone Institutes transformed skin cells into heart cells and brain cells using a combination of chemicals.
Detection of HPV in First-Void Urine
Similar sensitivity of HPV test on first void urine sample compared to cervical smear.
Potential “Good Fat” Biomarker
New method to measure the activity of energy consuming brown fat cells could ease the testing weight loss drugs.
Shape Of Tumor May Affect Whether Cells Can Metastasize
Illinois researchers found that the shape of a tumor may play a role in how cancer cells become primed to spread.
MicroRNA Pathway Could Lead to New Avenues for Leukemia Treatment
Cancer researchers at the University of Cincinnati have found a particular signaling route in microRNA (miR-22) that could lead to targets for acute myeloid leukemia, the most common type of fast-growing cancer of the blood and bone marrow.
Analysis of Dog Genome will Provide Insight into Human Disease
An important model in studying human disease, the non-coding RNA of the canine genome is an essential starting point for evolutionary and biomedical studies – according to a new study led by The Genome Analysis Centre (TGAC).
New Blood Test for The Earlier Diagnosis of Breast Cancer Spread
Researchers at University of Westminster have confirmed that a new blood test can detect if breast cancer has spread to other parts of the body.
First Gene Therapy Successful Against Human Aging
American woman gets biologically younger after gene therapies.
Targeting an ‘Undruggable’ Cancer Gene
RAS genes are mutated in more than 30 percent of human cancers and represent one of the most sought-after cancer targets for drug developers.
Altered Metabolism of Four Compounds Drives Glioblastoma Growth
Findings suggest new ways to treat the malignancy, slow its progression and reveal its extent more precisely.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!